Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Liquid Cultures   Original Sensible Seeds High THC Strains   PhytoExtractum Kratom Powder for Sale   North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Extract

Jump to first unread post Pages: 1
Offlineredpillcottage
Mr Fantastic
Male User Gallery


Registered: 07/28/20
Posts: 45
Loc: United Kingdom Flag
Last seen: 2 years, 11 months
Correct agar technique - please advise me. spore syringe to agar
    #26856496 - 08/01/20 12:25 AM (3 years, 5 months ago)

Hey there.

Can someone point me in the right direction for the proper technique to go from spore syringe to agar?

So I vaguely remember how to isolate a pure culture way back when i did biology at uni. so you get the bacteria, fungus, or whatever sample and wipe it on the side of the agar dish, flame the loop, drag a corner across, flame the loop drag the corner. eventually, you start to get isolated cultures from one bacteria or in this case a spore. Is this something I want to do?



--------------------
====================================


We're all going to die


Extras: Filter Print Post Top
Invisiblec10h12n2o
serial dilutor
 User Gallery


Registered: 01/21/15
Posts: 3,200
Loc: the abyss
Re: Correct agar technique - please advise me. spore syringe to agar [Re: redpillcottage]
    #26856513 - 08/01/20 12:37 AM (3 years, 5 months ago)

Check my sig, there is a section on streaking spore syringes to agar. "Working with spores"


--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche


Extras: Filter Print Post Top
Invisiblecoversall
إِنْ شَاءَ ٱللَهُ
 Unread Journal


Registered: 06/06/20
Posts: 2,749
Loc: संसार
Re: Correct agar technique - please advise me. spore syringe to agar [Re: redpillcottage]
    #26856514 - 08/01/20 12:38 AM (3 years, 5 months ago)



--------------------

..:: E V E R Y  ::..

..:: New? Start here. ::..
..:: How I Panaeolus. From Agar to Tea ::..


Extras: Filter Print Post Top
Invisiblesandman420
Saint PP
Male


Registered: 06/17/04
Posts: 5,384
Trusted Cultivator
Re: Correct agar technique - please advise me. spore syringe to agar [Re: coversall]
    #26856836 - 08/01/20 08:24 AM (3 years, 5 months ago)

you just have to squeeze out a bit after flaming the needle to get the plunger loosey goosey before trying to shoot one, else a big rush will come out...

So flame, squirt as little as possible into a sacrificial container to feel the plunger loosen up, barely press the plunger to make 1 single micro-drop on each plate of the stack without flaming anymore in between just move up the sleeve from the bottom boop boop boop.

Tilt it around a bit after wrapping the plate thats it.

Make your agar a little sofgter than normal too, like 14g instead of 20g per liter.

Do at least 10-20 plates to get a some good ones.

May as well just do a whole sleeve in one go.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed


Edited by sandman420 (08/01/20 08:29 AM)


Extras: Filter Print Post Top
Invisiblec10h12n2o
serial dilutor
 User Gallery


Registered: 01/21/15
Posts: 3,200
Loc: the abyss
Re: Correct agar technique - please advise me. spore syringe to agar [Re: sandman420]
    #26857344 - 08/01/20 02:10 PM (3 years, 5 months ago)

I much prefer serial streaking over just rolling the liquid around. The pic op posted is exactly what i do

It gives you waaaaay more to choose from, way more organized growth with less strains per colony in the later zones

But whatever works works


--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche


Edited by c10h12n2o (08/01/20 02:11 PM)


Extras: Filter Print Post Top
OfflineCatsLoveHouseMusic
SpeakerFreaker
 User Gallery


Registered: 11/03/18
Posts: 1,085
Last seen: 1 year, 7 months
Re: Correct agar technique - please advise me. spore syringe to agar [Re: c10h12n2o]
    #26857366 - 08/01/20 02:34 PM (3 years, 5 months ago)

I was just going to post this guys tek, the guy who posted above me. I do a mix of this guys tek^^^^ and a simple drop in the middle of agar. See what happeneds. Just be really sanitary, I go above what more probably call sanitary and my agar always works out.


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Mushroom-Hut Liquid Cultures   Original Sensible Seeds High THC Strains   PhytoExtractum Kratom Powder for Sale   North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Extract


Similar ThreadsPosterViewsRepliesLast post
* spore syring is in trouble? mulan 14,122 12 08/29/09 09:15 PM
by HaRo On KoRn
* Spore Syring Cont/Clear Misconcept if any Zwieback0 5,674 4 08/11/08 03:22 AM
by fr33d0mfry
* Re: When buying a spore syring... 3Mshroom 7,932 2 06/01/00 08:17 PM
by 3Mshroom
* Re: Antibiotic Agar Mix for Spore Germiniation in Water? Anonymous 1,524 1 05/09/00 06:46 PM
by Anonymous
* Agar: What Am I Doing Wrong??
( 1 2 all )
wolfXsoul 424 22 08/01/20 03:26 PM
by wolfXsoul
* Benefits of Agar? celsius 9,381 10 12/01/19 12:36 AM
by wildernessjunkie
* Re: agar questions Anonymous 1,400 6 05/30/00 03:07 PM
by Anonymous
* Re: Spore transfer? Anonymous 1,901 1 03/08/01 08:17 PM
by Juggles

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,074 topic views. 40 members, 244 guests and 43 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.021 seconds spending 0.007 seconds on 12 queries.