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redpillcottage
Mr Fantastic



Registered: 07/28/20
Posts: 45
Loc: United Kingdom
Last seen: 2 years, 11 months
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Correct agar technique - please advise me. spore syringe to agar
#26856496 - 08/01/20 12:25 AM (3 years, 5 months ago) |
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Hey there.
Can someone point me in the right direction for the proper technique to go from spore syringe to agar?
So I vaguely remember how to isolate a pure culture way back when i did biology at uni. so you get the bacteria, fungus, or whatever sample and wipe it on the side of the agar dish, flame the loop, drag a corner across, flame the loop drag the corner. eventually, you start to get isolated cultures from one bacteria or in this case a spore. Is this something I want to do?
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==================================== We're all going to die
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: Correct agar technique - please advise me. spore syringe to agar [Re: redpillcottage]
#26856513 - 08/01/20 12:37 AM (3 years, 5 months ago) |
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Check my sig, there is a section on streaking spore syringes to agar. "Working with spores"
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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coversall
إِنْ شَاءَ ٱللَهُ



Registered: 06/06/20
Posts: 2,749
Loc: संसार
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Re: Correct agar technique - please advise me. spore syringe to agar [Re: redpillcottage]
#26856514 - 08/01/20 12:38 AM (3 years, 5 months ago) |
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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,384
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Re: Correct agar technique - please advise me. spore syringe to agar [Re: coversall]
#26856836 - 08/01/20 08:24 AM (3 years, 5 months ago) |
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you just have to squeeze out a bit after flaming the needle to get the plunger loosey goosey before trying to shoot one, else a big rush will come out...
So flame, squirt as little as possible into a sacrificial container to feel the plunger loosen up, barely press the plunger to make 1 single micro-drop on each plate of the stack without flaming anymore in between just move up the sleeve from the bottom boop boop boop.
Tilt it around a bit after wrapping the plate thats it.
Make your agar a little sofgter than normal too, like 14g instead of 20g per liter.
Do at least 10-20 plates to get a some good ones.
May as well just do a whole sleeve in one go.
Edited by sandman420 (08/01/20 08:29 AM)
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: Correct agar technique - please advise me. spore syringe to agar [Re: sandman420]
#26857344 - 08/01/20 02:10 PM (3 years, 5 months ago) |
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I much prefer serial streaking over just rolling the liquid around. The pic op posted is exactly what i do
It gives you waaaaay more to choose from, way more organized growth with less strains per colony in the later zones
But whatever works works
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
Edited by c10h12n2o (08/01/20 02:11 PM)
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CatsLoveHouseMusic
SpeakerFreaker



Registered: 11/03/18
Posts: 1,085
Last seen: 1 year, 7 months
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Re: Correct agar technique - please advise me. spore syringe to agar [Re: c10h12n2o]
#26857366 - 08/01/20 02:34 PM (3 years, 5 months ago) |
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I was just going to post this guys tek, the guy who posted above me. I do a mix of this guys tek^^^^ and a simple drop in the middle of agar. See what happeneds. Just be really sanitary, I go above what more probably call sanitary and my agar always works out.
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