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Offlineredpillcottage
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Very Slow Colonisation - spore syringe | rye grain *Pics*
    #26850025 - 07/28/20 02:20 PM (3 years, 6 months ago)

Hi there. Hope you guys can give me a little advice here. Not sure if I'm doing something wrong here.
This is the second time I've ever grown shrooms. the first being way back in UK utopia days when you could still buy fully colonised kits.

Its now the 27th July and I injected spores into rye grain on the 5th of July. so 22 days ago. The important thing is that the room has been a steady 21 - 22 deg C the whole time. I might float it a tub of water heated with an aquarium heater. what do you think?

I got this kit online. seems like a good company from Scotland. Rye grain with what looks like choir and gypsum apparently. 6 self-healing injection ports, air filter strips. and verm cap. All looks pretty good. + a bunch of accessories blar blar. 

Injected 1 ml of a 3 ml syringe into 3 different ports and only one site has colonised. I think that's because there was a big clump of spores. i figure it will still work but its taking ages

Thanks for the help.



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Offlinenormalperson
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Re: Very Slow Colonisation - spore syringe | rye grain *Pics* [Re: redpillcottage]
    #26850266 - 07/28/20 04:52 PM (3 years, 6 months ago)

the temperature is fine but that looks like bacteria to me. 22 days is not a long, long time to wait for spores to sprout, give everything a little more time.


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Offlineredpillcottage
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Re: Very Slow Colonisation - spore syringe | rye grain *Pics* [Re: normalperson]
    #26850996 - 07/29/20 12:51 AM (3 years, 6 months ago)

Quote:

normalperson said:
the temperature is fine but that looks like bacteria to me. 22 days is not a long, long time to wait for spores to sprout, give everything a little more time.




Thanks for the reply. My gut says it's not bacteria. Very white ropey mycelium. But I'll keep my eye on it.


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OfflineYour Dudeness
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Re: Very Slow Colonisation - spore syringe | rye grain *Pics* [Re: redpillcottage]
    #26852898 - 07/30/20 01:28 AM (3 years, 6 months ago)

It looks too wet on the bottom half I don't know why grow kits and bags arrive too wet causing bacterial contam issues.  From what I've seen on this forum if you made that kit yourself at home it'd be almost ready by now.  throw the white chunk in the garden or try a new batch.  You can wait the 3-4 months for it to colonize with wet substrate but where is the fun in that?


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Offlineredpillcottage
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Re: Very Slow Colonisation - spore syringe | rye grain *Pics* [Re: Your Dudeness]
    #26852908 - 07/30/20 02:02 AM (3 years, 6 months ago)

Yer. bit rubbish ey. It seems difficult to get good supplies with all the COVID stuff. I think I will throw it in the wardrobe and forget about it. I have a lot more stuff in the mail. I'm going to make my own brf-tek I just need a good spore supply. I have actually ordered some and paid 20£ but they are not responding to email. going to call them in a moment actually. ahhh the psychedelic business world, got to love it ey. lol

thanks for the reply.


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OfflineEntheoGod
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Re: Very Slow Colonisation - spore syringe | rye grain *Pics* [Re: redpillcottage]
    #26852964 - 07/30/20 04:54 AM (3 years, 6 months ago)

Try colonizing some spawn before making it into a tray for fruiting.

You want spawn colonized clean before anything else. I feel like this is trying to skip too many steps.

Kits are trash. Try to avoid them. I dont believe in anything premixed like that, staying sterile long enough for you to have any use out of it. I could be wrong though.

🤷‍♂️

My advice is colonize some grains first or even better, just start working on agar and clean up a MS syringe or spore print then put that to grain so you know you dont have any contams to start with. Not saying this is contaminated but theres a LOT of posts on here about failed grow kits.


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OfflineYour Dudeness
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Re: Very Slow Colonisation - spore syringe | rye grain *Pics* [Re: EntheoGod]
    #26853763 - 07/30/20 02:32 PM (3 years, 6 months ago)

use the shroomery sponsors some of them have been satisfying mycologists for 10 years.  I wish kits were delivered without so much water content it'd make things easier for the beginners.  With so many vendors you might get lucky using kits.


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Offlineredpillcottage
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Re: Very Slow Colonisation - spore syringe | rye grain *Pics* [Re: EntheoGod]
    #26853834 - 07/30/20 03:15 PM (3 years, 6 months ago)

So I have 4 spores syringes in the post. it's from the best company in the UK I'm sure of that much.

My plan Is to use one to colonise my own brf-tek setup. simple box system similar to the jar method.

Use another on some sterilised liquid culture medium. they are little sealed jars similar to a medicine bottle–just inject spores into the self-healing port.  also in the mail. if that works, ill use it to colonise a bigger brf box that I sterilise myself. 

That leaves me two more incase it all goes wrong and I have to rethink the whole plan.

fingers crossed


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Offlineredpillcottage
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Re: Very Slow Colonisation - spore syringe | rye grain *Pics* [Re: redpillcottage]
    #26854804 - 07/31/20 02:35 AM (3 years, 6 months ago)

************************************************************************

************************************************************************


UPDATE


After a lot more research... the agar is the way to go. 1 million percent. wish I had gone that way first but I guess you have to finger these things out.

Use the good spores in the post. grow out on agar. store some. use some on brf , grain sporn, my wife apple pie, whatever really because agar is the shit and I will have plenty to play with.


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InvisibleGoatrider
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Re: Very Slow Colonisation - spore syringe | rye grain *Pics* [Re: redpillcottage]
    #26854826 - 07/31/20 03:16 AM (3 years, 6 months ago)

That`s it mate :thumbup:

Remember that strict borders from
colonized to uncolonized for the future.
That`s a true sign for contam.
So you took some lesson out of kits :wink:


                  :cookiemonster:


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OfflineYour Dudeness
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Re: Very Slow Colonisation - spore syringe | rye grain *Pics* [Re: Goatrider]
    #26855531 - 07/31/20 01:09 PM (3 years, 6 months ago)

You can have all the agar you want if the substrate is too wet you'll have the same crappy colonization every time.  Maybe RR could pull it off but only him!


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