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Offlinehardinthekitchen
back again for the first time :P

Registered: 02/11/20
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Mazatapec - MS > Grain > Agar / G2G * 1
    #26848072 - 07/27/20 02:14 PM (3 years, 6 months ago)

Hey everyone :smile:

Sometime in June inoculated some oat jars with a bunch of MS spore syringes. The Mazatapec has been doing OK! I wanted to update and ask for some wisdom.

Initially I went from MS syringe to grain. From there I went G2G





and put a colonized grain on an LME agar plate.



The agar looks great to me but something looks off the with G2Gs..what do you guys think??


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Offlineskullhuman
the skullman cometh
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Registered: 06/15/09
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Re: Mazatapec - MS > Grain > Agar / G2G [Re: hardinthekitchen]
    #26848121 - 07/27/20 02:36 PM (3 years, 6 months ago)

I think you found some real nice growth to make some cultures out of.  Good job!


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OfflineA.k.aM
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Registered: 10/27/19
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Re: Mazatapec - MS > Grain > Agar / G2G [Re: skullhuman]
    #26848148 - 07/27/20 02:53 PM (3 years, 6 months ago)

The jars look ok actually imo. I’ve done a lot of spore to grain grows but I always consider it to kinda have a clock on it since you can’t be sure it’s clean. If there is contam in there g2g will massively increase the chances it’ll get a foothold and ruin things.

Maz has always been really fast for me though so if I had to pick one to do this with it would be maz.

That’s a nice plate too.


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LAGM2020


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OfflineBroken Veils
Something


Registered: 04/26/20
Posts: 41
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Last seen: 3 years, 1 month
Re: Mazatapec - MS > Grain > Agar / G2G [Re: A.k.a]
    #26848633 - 07/27/20 07:22 PM (3 years, 6 months ago)

Everything looks pretty good...agar looks solid.  A.K.A is right on with the G2G advice...


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Offlinehardinthekitchen
back again for the first time :P

Registered: 02/11/20
Posts: 16
Last seen: 3 years, 4 months
Re: Mazatapec - MS > Grain > Agar / G2G [Re: Broken Veils]
    #26853683 - 07/30/20 02:10 PM (3 years, 6 months ago)

Hey thanks for the support you guys! Thought the jars were kind of goners :sun:

Out of the agar transfers, only one is looking as decent as the original plate it was taken from. With that news was thinking that I may wait until I build a flow hood until I try and go back to the original plate. I took two grains from same MS solution but different jars and did more plates, they are looking healthy!

I'll update this with some pics of some agar in grain and some tubs when I get there....don't feel like cracking the plates open again without the hood.


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