Home | Community | Message Board

Out-Grow.com - Mushroom Growing Kits & Supplies
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Kraken Kratom Red Vein Kratom

Jump to first unread post Pages: 1
OfflineAlpineHighs
Thrill Shrieker

Registered: 04/22/20
Posts: 87
Loc: Montucky
Last seen: 1 year, 6 months
Early Contamination? Again?!?!
    #26853394 - 07/30/20 11:43 AM (3 years, 6 months ago)

Hey guys,

I inoculated a bunch of oat jars with agar wedges on 7/23, and it looks like my second attempt in two months is probably already contaminated. Or are they??

4 jars of B+ clone => agar ; 6 PE clone => agar ; 9 PE pin clone => agar

And every...single...jar looks like this after 7 days. I was worried about the weak/whipsy growth over the past couple days. This morning I smelled the GE hole of one with an SFD, and it smelled kinda similar to the 45 stank ass sour jars that I had to toss out a few weeks ago. So I took a couple away from my zone and opened them, and I'm 84.333% sure there was a distinct sour smell. There are patches of new rhizo growth on the bottom leading edge in every jar, but all jars have that weak/whispy growth below the grain surface, and fuzzy shit on top.



Oats were PCooked for 1hr 45min. Lids have correct size GE hole, with either an SFD or Bod's polyfil lids. I use a still air box. Proper sterile technique. All agar dropped to grain was on its 3rd transfer. All mycelium from each plate I used was this clean and rhizomorphic....



Is it worth shaking these jars to see if they recover? Or should I get them out of here ASAP, clean the fuck out of my apartment, and hope for better luck with round #3 of 2020?

I keep shaking and smelling the 2 I opened, and still can't definitively tell if they're sour. But the fuzzy stuff on top sure didn't look like mycelium when I opened them.

What the hell am I doing wrong???


Extras: Filter Print Post Top
OfflineAlpineHighs
Thrill Shrieker

Registered: 04/22/20
Posts: 87
Loc: Montucky
Last seen: 1 year, 6 months
Re: Early Contamination? Again?!?! [Re: AlpineHighs]
    #26853580 - 07/30/20 01:26 PM (3 years, 6 months ago)

Anyone have any suggestions? I guess I'll plan to shake these jars and hope for the best.

I have these 3 PE T2 plates that I'm probably gunna use to inoculate more jars tomorrow. They look clean, but maybe dirty plates are the source of contams in my grain jars?



I know I should avoid the edges when cutting wedges from a full plate, but should I also avoid the center where the transfer wedge is? I have been using 1 plate (mostly full) cut into 4 equal segments for each jar. Is it bad practice to inoculate jars with the center bit that has the transfer wedge on it?


Extras: Filter Print Post Top
Offlinejay.achS
Traveler
Male User Gallery


Registered: 05/25/19
Posts: 531
Loc: USDA Zone 8
Last seen: 18 days, 11 hours
Re: Early Contamination? Again?!?! [Re: AlpineHighs]
    #26854332 - 07/30/20 08:15 PM (3 years, 6 months ago)

Did you hydrate the oats before you PC'ed them?  What tek did you use?

These are the oat teks I have tried:

Bods EAF
https://www.shroomery.org/forums/showflat.php/Number/24126032

Shaping Dreamers -> 25 at boil.
https://www.shroomery.org/forums/showflat.php/Number/26005814

Eats (Method 1 only): Oats definitely damp going into jar but I had the best results with this one
https://www.shroomery.org/forums/showflat.php/Number/22158748

Socks
https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26617604&page=0&vc=1#26617604

Inocuole
https://www.shroomery.org/forums/showflat.php/Number/21029762#21029762


--------------------
“One's destination is never a place, but a new way of seeing things.”  - Henry Miller

LAGM v. 2.023
LAGM2021


Extras: Filter Print Post Top
OfflineAlpineHighs
Thrill Shrieker

Registered: 04/22/20
Posts: 87
Loc: Montucky
Last seen: 1 year, 6 months
Re: Early Contamination? Again?!?! [Re: jay.ach]
    #26855702 - 07/31/20 03:15 PM (3 years, 6 months ago)

Yeah I used Bod's oat prep tek, and even cut down on the boil time to make damn sure they weren't too saturated. As soon as I saw a few grains burst, I strained them and laid them out on a folding table to steam dry for almost 2 hrs. PC @ 15 PSI for 2 hrs.

I'm stumped as to which part of the process all this contamination is coming from. Lately I've lost well over 50 jars to sour rot; I think 4 or 5 different PC runs and SAB inoculation sessions.

I've been setting my SAB directly on a wood table after dousing every sq cm of surface area with 70% iso. Also using 1 or 2 blank petri dishes as the "floor" for all dishes I'm working with. I'll plan to use the lid of my SAB as the floor for this evening's inoculation sesh and see if that solves the issue?

Fingers crossed


Extras: Filter Print Post Top
OfflineFuddyduddy
Stranger
 User Gallery
Registered: 04/29/20
Posts: 34
Last seen: 3 years, 1 month
Re: Early Contamination? Again?!?! [Re: AlpineHighs]
    #26855743 - 07/31/20 03:50 PM (3 years, 6 months ago)

Possibly underhydrated the oats..?
If not fully hydrated & part of the PC cycle was used to finish cooking the oat to the center then perhaps not all of the natural bacteria was killed.

I cooked a batch just as you described and it was the only time I had bacteria in my jars. Cooking them until more oats burst has not lead to any problems for me. 40-45 min boiling


Extras: Filter Print Post Top
OfflineAlpineHighs
Thrill Shrieker

Registered: 04/22/20
Posts: 87
Loc: Montucky
Last seen: 1 year, 6 months
Re: Early Contamination? Again?!?! [Re: Fuddyduddy]
    #26855755 - 07/31/20 03:59 PM (3 years, 6 months ago)

Damn. That makes sense. I've got jars in the PC right now...should I maybe let it run for an extra 20-30 min @ 15 psi, for a total of 2.5 hrs, to try and make sure bacteria is done for?


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Kraken Kratom Red Vein Kratom


Similar ThreadsPosterViewsRepliesLast post
* rancid CrabbyAss 3,393 17 06/27/02 11:58 PM
by bluefaced
* Innoculated resterilized contaminated jar... Swami 3,271 8 04/10/18 11:44 AM
by Shroomarican
* Re: Which is the best way to clean contaminated jars to reuse them? n8 3,893 2 01/05/00 01:13 PM
by sutec
* contaminated jar rejuvination fig 2,708 4 03/02/02 06:34 PM
by canid
* contaminated jar rejuvination... fig 2,001 4 03/01/02 10:45 PM
by MushyMay
* Re: cleaning contaminated jars Psychonaut 3,170 1 05/24/00 04:06 PM
by PhilosoPossum
* disposal of contaminated jars? Shiznitz 3,547 8 08/06/01 09:15 AM
by dopemaster
* Contaminated Jar Recovery. motatraehrehtom 1,844 4 12/16/02 12:48 PM
by motatraehrehtom

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
230 topic views. 26 members, 130 guests and 69 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.022 seconds spending 0.006 seconds on 12 queries.