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SoupyGeorge
Hazy Daze since First Grade



Registered: 05/25/20
Posts: 216
Loc: Southern US of 'Merica
Last seen: 1 year, 6 months
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Re: Last bits uncolonized/stalling [Re: otsilla]
#26854982 - 07/31/20 07:29 AM (3 years, 5 months ago) |
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Well you mentioned it being "too wet from condensation". My guess is your grains were too wet form the get go. My healthiest jars never get the first drop of condensation on the inside. If you can visibly see water on the inside of the jar that's enough to be skeptical in my opinion. Those jars go straight to my outdoor patch for nature to deal with. I have enough grows staggered between harvest that I can start getting a little more picky with my jars though. On that note, always try to have mushrooms at every stage of development.
1)Spore prints. 2)Empty sterile Agar plates (for clones and prints) 3)1st generation Agar plates (from clones and prints) 4)Steadily transfer to clean said plates. 5)As soon as you have a clean plate put it to grain or if you're overwhelmed with too much grain at the moment put it in a fridge to halt until you are ready to inoculate with it. 6)Always Grain to Grain. I call it spawn math at this point in my hobby, if I want to spawn 5 boxes I make 8 jars. Giving my human self room for error and contam to take 3 jars. If it doesn't awesome, I use those to Grain to Grain again and expand for he next grow. 7)Which leads to this step. Having multiple boxes at different stages of growth, harvesting 2 or 3 boxes a week. Always assuming they won't make it to the 2nd flush. This is the step I'm currently trying to fulfill. I have 3 staggered boxes at this point. Next week I will spawn my next boxes and boom, I'll be in full rotation at that point.
When I first started I spawned a jar with obvious contamination (slight green coloration) in a shoebox. I kept it outside in my shed instead of in my house where my other shoeboxes were. It fruited, 2 flushes, if you can call them that.

The second "flush" gave me 5 more shrooms and that was it. So from that experience the possibility of getting enough weight off of a contaminated jar is not worth the time and effort put into it.
(Bad advice right here) I know not everyone who is new has fruits and just want to get their first fruits so its possible to spawn that jar if you are desperate, but keep it outside in another area just in case it goes south.
(Good advice right here) Keep working those jars. Take a piece of colonized grain off top and put it to agar like someone said earlier in the thread. Clean the culture with transfers. Then drop the clean agar plate into a new jar use good clean technique. From there use Grain to Grain to expand your spawn.
Last thing to say. If you have the opportunity, spawn those contaminated jars outside in an outdoor bed. Your going to want an outdoor bed for spent substrates anyway, as a means to have a continually flushing harvest. Its better than throwing them away when they still have life to give.
As far as future reference, I don't know how you prepare grain, but I prefer bod's boil technique. Boil whole grain for 30 minutes and then let it dry on a big window screen. The trick is to let it dry "too much" you want to think, damn that's too dry, when you run your hand through it. You should be able to scoop up a handful and place it on a paper towel, wait a few seconds and dump it off leaving a perfectly dry paper towel.
As far as Agar plain Light Malt Extract Agar work, not need for the anti bacterial shit. Just use good clean procedure found in the SAB teks on this sight.
-------------------- Mellow heads will prevail. You look like everyone else because the T.V. tells you to. I look like everyone else so that i don't stand out to the man. We are not the same.
Edited by SoupyGeorge (07/31/20 07:34 AM)
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Roger Clemency
Smile


Registered: 03/23/20
Posts: 2,005
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Re: Last bits uncolonized/stalling [Re: otsilla]
#26855224 - 07/31/20 10:12 AM (3 years, 5 months ago) |
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Quote:
otsilla said: Thanks for sharing that. So what do you think was at fault, the fact that I didn't shake, and let it sit enough that bacteria started growing from the farest end? Or was it always there and they would've gone bad anyways?
I have only a very small portion of not so clean spores left, what would be the best bet, Antibiotic agar and a couple of transfers?
Did you start these jars from clean agar? If so then you have to take a look at your technique in inoculating, or study your plates closer. If you used a spore syringe it’s always tough to say because spores to grain can work or they can contam and you won’t know if it was because of the syringe or your technique.
Not shaking won’t directly cause problems but I look at growing cubes like a race. We can’t really kill 100% of everything I don’t think during a PC cycle but if done correctly you deliver a serious knockout blow. While whatever might still be there is weak and almost nonexistent you need your myc to take over as quick as possible. The longer it takes the stronger other things still in there (or maybe that got in during inoculation) will become. This is why I’d always advise against spore syringe to grain and most especially to a large grain bag. You want live mycelium to be able to take off right away.
I use LI for all my jars now and even the first time I tried it (munchauzen’s blenderless LI tek) I ended up having to use a germ plate, and I messed up and had to drill a hole in my lid to release pressure and then I dropped the agar puck in, covered the hole with a bandaid and shook it up with water coming out everywhere, sure it would fail. I nocc’d up my jars anyway and they were all 100% in 10-13 days. All subs made it to the first flush at least but about half did start showing signs of trich after the first.
Shitty technique and planning but speed won out. If you have plates and still haven’t tossed those jars you could pluck a grain onto agar like someone mentioned above. You might be able to clean it up from there. I don’t have any experience though with antibiotic agar so Idk but I think if you’re careful, and look up “serial dilution” you can get clean myc from your spores.
C12ch1597 or something like that - the guy with an avatar of Putin in a face shield - has a write up and little diagram of how to swipe spores in this way. You basically put them in one spot then swipe away from there a few times, then sterilize your loop and without scraping your print again you turn the plate 90* and start a new swipe from where your first swipe ended, . Basically dragging the spores out further and further and contams and cube spores will get left along the path, hopefully to germinate in separate areas.
-------------------- Sour grapes, sweet revenge Heaven starts right where hell ends
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