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Offlineotsilla
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Last bits uncolonized/stalling
    #26852930 - 07/30/20 03:00 AM (3 years, 5 months ago)

Hey,

So, I have couple of rye jars which are about 1-1.5 month old now, never been shaken, but the very last bits seem to be stalling, a bit too wet also, so i turned them upside down hoping that it'll finish them, but still no change after 24h, what should I do, shake, spawn all but the uncolonized bits or wait for them to fully colonize?









As you can see on some of the pictures, the last bit is lighter mycelium, this is the last weeks process, the thick white line is where it was before, no change after 24h after the flip tho. And the smell coming through the GE hole is the right mushroom smell.


Edited by otsilla (07/30/20 03:05 AM)


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Re: Last bits uncolonized/stalling [Re: otsilla]
    #26852953 - 07/30/20 04:29 AM (3 years, 5 months ago)

Those are fucked.


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Offlineotsilla
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Re: Last bits uncolonized/stalling [Re: otsilla]
    #26852988 - 07/30/20 05:44 AM (3 years, 5 months ago)

why exactly? nothing to salvage, simply to get a mushroom to get a clone?


Edited by otsilla (07/30/20 05:49 AM)


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OfflineEntheoGod
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Re: Last bits uncolonized/stalling [Re: otsilla]
    #26853005 - 07/30/20 06:15 AM (3 years, 5 months ago)

Just start over. At this point that’s your best option unfortunately. You live and you learn. 😁

Good luck on future attempts!


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Offlineotsilla
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Re: Last bits uncolonized/stalling [Re: otsilla]
    #26853016 - 07/30/20 06:35 AM (3 years, 5 months ago)

any particular reason tho why they are all fucked beyond no return, or at least what happened, to fix it next time, all stalled like this on the bottom, strange, other than that, looks healthy myc to me :confused:


Edited by otsilla (07/30/20 06:42 AM)


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OfflineSoupyGeorge
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Re: Last bits uncolonized/stalling [Re: otsilla]
    #26853087 - 07/30/20 07:24 AM (3 years, 5 months ago)

I could be wrong but I believe a hard stall line, such as that, where it seems the mycelium meets a border and cannot surpass it is a sign of contamination.

Quote:

Kizzle said:
The following guide is unfinished. Feel free to offer information, high quality pictures or to correct any misinformation. Plenty of guides like this have been posted in the past. The difference here is this one will focus on Cubensis in particular although most of the information could apply to any mushroom species being grown.

Signs of Contamination


Sectoring - When two different fungal species are growing in the same substrate they tend to form distinct borders between the two mycelia. Often a line of metabolites may be seen where the two fungi meet. Hourglass shaped mold sectors in PF jars are almost always the result of a compromised dry vermiculite layer whereas circular mold colonies are usually the result of a mold growing from one of the inoculation points.




This is from the "Recognizing and dealing with contamination" thread in the cultivation forum.


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Offlineotsilla
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Re: Last bits uncolonized/stalling [Re: SoupyGeorge]
    #26853116 - 07/30/20 08:04 AM (3 years, 5 months ago)

Sure, I get that, but there doesn't seem to be that kind of a hard stall, what I mean that I thought there was, since I get back home(was away for few weeks) it was at the red line, I thought it stalled and decided to wait a bit to see, so now after a week has passed, it has gone from the red line to the grey one, true, a bit weaker myc, but still seems myc to me. So I thought it was due to the bottom being too wet from condensation, so I turned those jars upside down just a day ago, thinking that maybe that fill speed up the last part.

So thats why I want to know what seems to be that off that I need to toss them all, wouldn't it be possible to not just spawn that part and use the rest, healthy looking myc?



Edited by otsilla (07/30/20 08:07 AM)


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Re: Last bits uncolonized/stalling [Re: otsilla]
    #26853117 - 07/30/20 08:08 AM (3 years, 5 months ago)

Quote:

otsilla said:
any particular reason tho why they are all fucked beyond no return, or at least what happened, to fix it next time, all stalled like this on the bottom, strange, other than that, looks healthy myc to me :confused:





It does NOT look like healthy mycelium it doesn't even look like cubensis mycelium. If it is cube mycelium it is totally infected with a white mold tag-along contam. Too bright. Too dense. Too fuzzy at the ends.

Not to mention the bacterial contam keeping the parts from colonizing.


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OfflineEntheoGod
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Re: Last bits uncolonized/stalling [Re: otsilla]
    #26853118 - 07/30/20 08:09 AM (3 years, 5 months ago)

If youre having to ask us why its stalled, then you can clearly tell something is wrong. The chances are its gone bacterial.

Likely due to standing water if you said you flipped it because you had too much water in the bottom. But regardless it doesnt look healthy. The mycelium itself looks almost moldy. If you open it later and it smells fine, then id be willing to say maybe we are wrong but I doubt thats going to happen. Learn from the mistakes and improve for the future.

You got this. If you want, go ahead and let those finish colonizing and see what they look and smell like. I dont think it would be worth the wait though.


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Re: Last bits uncolonized/stalling [Re: otsilla]
    #26853121 - 07/30/20 08:12 AM (3 years, 5 months ago)

If you can separate the contaminated part from the rest, it could be possible to use things without making the other stuff contaminated also...
I'm doing normal BRF cakes in a jar where entire cake can come out and when there's a last bit left that won't colonize I simply cut it off with some margin, wash the area thoroughly and let it fruit and things work out nicely but with grain I'm not sure how feasible this would be.


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Re: Last bits uncolonized/stalling [Re: MindMeower]
    #26853125 - 07/30/20 08:16 AM (3 years, 5 months ago)

That is totally no feasible for grain spawn. Sure you can trim a cake as a last resort I guess. But this stuff is going to be mixed up with a larger substrate to expand its mass and it will bring the nasty to the larger mass.


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Offlineotsilla
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Re: Last bits uncolonized/stalling [Re: EntheoGod]
    #26853135 - 07/30/20 08:31 AM (3 years, 5 months ago)

Quote:

EntheoGod said:
If youre having to ask us why its stalled, then you can clearly tell something is wrong. The chances are its gone bacterial.

Likely due to standing water if you said you flipped it because you had too much water in the bottom. But regardless it doesnt look healthy. The mycelium itself looks almost moldy. If you open it later and it smells fine, then id be willing to say maybe we are wrong but I doubt thats going to happen. Learn from the mistakes and improve for the future.

You got this. If you want, go ahead and let those finish colonizing and see what they look and smell like. I dont think it would be worth the wait though.




Alright, thank you, I'm quite sure that it smells fine, and also my myc has always been quite fluffy and moldy though being fine and producing multiple flushes.

pictures from my last spawn:



I think I'm going to remove the caps, give them a good smell, if they smell anything off I'll toss them, but if they smell good, I think I'm gonna try to break the glass to get them out as whole, then give the uncolonized parts a sniff to maybe get an idea, and separate them, by cutting or maybe breaking off seems even better and give them a rinse and still try bulk just to see what might or might now happen.

And start again from agar I guess :laugh:


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Re: Last bits uncolonized/stalling [Re: otsilla]
    #26853174 - 07/30/20 09:18 AM (3 years, 5 months ago)

Another thing you can do as a back-up in case your projects with these jars fail is to pluck a single colonized grain from the jar and put it on agar.  You can then proceed to clean up the culture instead of having to start from spores again.


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InvisibleRoger Clemency
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Re: Last bits uncolonized/stalling [Re: sandman420]
    #26853229 - 07/30/20 09:56 AM (3 years, 5 months ago)

Those jars do look very sketchy. I had an APE jar recently that had a spot where bacteria had obviously taken hold.

Luckily it was up top so I could scoop it out with everything else nearby. It looks fine now, fully colonized and thickening up in a shoe box and I’m hoping to at least get one flush out of it. If that jar smells like cube myc you may be able to do something similar and see what happens but I wouldn’t mix that with anything else.


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Re: Last bits uncolonized/stalling [Re: Roger Clemency]
    #26853278 - 07/30/20 10:24 AM (3 years, 5 months ago)

Looks bacterial or consolidated for way too long without being shaken.

I usually shake jars 2-3 times over the coarse of about 2 weeks to keep them chuggin along, nicely...


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Re: Last bits uncolonized/stalling [Re: LotKid]
    #26853305 - 07/30/20 10:40 AM (3 years, 5 months ago)

Now that a few TC's are here let me add something I read and see if they can confirm it. When a possibly contamed jar is shaken doesn't the contam keep it from recovering? And is this a way to test jars for contam? If it recovers fully and quickly it should be safe to use yeah?


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Re: Last bits uncolonized/stalling [Re: SoupyGeorge]
    #26853328 - 07/30/20 10:56 AM (3 years, 5 months ago)

Short answer... yes


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Re: Last bits uncolonized/stalling [Re: LotKid]
    #26853377 - 07/30/20 11:32 AM (3 years, 5 months ago)

nvm, every single one smelled sweet, back to the drawing board



thank you for your input tho :laugh:


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InvisibleRoger Clemency
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Re: Last bits uncolonized/stalling [Re: LotKid]
    #26853379 - 07/30/20 11:33 AM (3 years, 5 months ago)

2-3 days after a shake - where da bad one?

It did eventually colonize fully but you can tell it’s got some bacteria in there making it thick and unsightly. After a little shake and unscrewing the lid though it didn’t smell too bad.


Be careful though and pay close attention to all jars. I saw a small patch of grey mold on this jar to the right, I shook it anyway to watch it. It recovered a little slower than others but had I not known and not looked closely I may have let this one pass. Until smelling it anyway lol - it was gross.


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Offlineotsilla
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Re: Last bits uncolonized/stalling [Re: Roger Clemency]
    #26854803 - 07/31/20 02:34 AM (3 years, 5 months ago)

Thanks for sharing that. So what do you think was at fault, the fact that I didn't shake, and let it sit enough that bacteria started growing from the farest end? Or was it always there and they would've gone bad anyways?

I have only a very small portion of not so clean spores left, what would be the best bet, Antibiotic agar and a couple of transfers?


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