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Sherlock Shrooms
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Re: Please help me get this right, contamination blues.. [Re: MLPismyOPSEC]
#26875824 - 08/11/20 07:42 PM (3 years, 5 months ago) |
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Quote:
MLPismyOPSEC said: I've done about 100 tubs (~50 monos, ~40 shoeboxes), always cut the stipe, and never have contams because of it. I dump them after a third flush, but my first 30 monos and 20 SB's didn't contam at any point. I know it's not a lot of experience, but i feel pretty confident that the stumps don't cause contamination.
Thanks for the feedback. What size are your monos? As in the quart measurement of the whole tub. And what depth of sub do you normally do?
-------------------- "When you pass through the waters, I will be with you; And through the rivers, they shall not overflow you. When you walk through the fire, you shall not be burned, Nor shall the flame scorch you."

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MLPismyOPSEC
That One Ponyfucker


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Re: Please help me get this right, contamination blues.. [Re: Sherlock Shrooms]
#26878859 - 08/13/20 04:12 PM (3 years, 5 months ago) |
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32qt and 4" always, the mycelium always overgrows the stumps and the next flushes grow around em.
Edited by MLPismyOPSEC (08/13/20 04:13 PM)
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Sherlock Shrooms
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Re: Please help me get this right, contamination blues.. [Re: MLPismyOPSEC]
#26889930 - 08/20/20 11:24 AM (3 years, 5 months ago) |
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Interesting that your monos are all 32 quart. I consider my 30 quart size minis even though most here think of a 20 quart or so to be the standard mini size. All the problems I've been having are in 64 quart monos. Whenever I do a 30 quart tub I almost always get 2 flushes before seeing contams, and sometimes 3 or 4. It seems that the higher you go in size whether it be spawn containers or fruiting containers problems get amplified. Not sure if it's heat, moisture, air flow, or something else. I know many people use 50 and 60 quart monos and seem to be fine but I've got to follow what works for now. I have a bunch of 30 quarts going at the moment, most are midway through their 1st flush and a few have finished the first flush, no contams yet. We'll see how long they last..
-------------------- "When you pass through the waters, I will be with you; And through the rivers, they shall not overflow you. When you walk through the fire, you shall not be burned, Nor shall the flame scorch you."

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Sherlock Shrooms
Neophyte



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Re: Please help me get this right, contamination blues.. [Re: Sherlock Shrooms]
#26889937 - 08/20/20 11:34 AM (3 years, 5 months ago) |
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I am using pour epoxy on every interior surface of my flow hood. I even propped the flow hood at an angle \ and did a pour to make the transition from the floor/walls to the filter perfectly smooth. So there are 8 separate pours, 1 for each wall/floor and 1 for each angled transition to the filter. The inside of my flow hood work area will be like one smooth sheet of glass in all directions. I'll take pics and share them when it's done. Pour epoxy is cool stuff. The blow torches we use for flame sterilizing are the perfect thing for getting bubbles out of the epoxy after it's poured. Hold the flame 6 or so inches away and do brief passes back and forth to pop all the bubbles and get a perfectly smooth finish, do that 2 or 3 separate times within 30 or 40 minutes of pouring Just be careful not to light the hepa filter on fire I used metal flashing to create a flame/heat barrier in the areas I was torching..
-------------------- "When you pass through the waters, I will be with you; And through the rivers, they shall not overflow you. When you walk through the fire, you shall not be burned, Nor shall the flame scorch you."

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ricelick
Barkeep
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Re: Please help me get this right, contamination blues.. [Re: Sherlock Shrooms]
#27076425 - 12/07/20 01:27 AM (3 years, 1 month ago) |
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Did you ever get this figured out? How did that last tub turn out?
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teeter
Mindfucked



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Re: Please help me get this right, contamination blues.. [Re: ricelick]
#27162654 - 01/21/21 11:36 PM (3 years, 6 days ago) |
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Quote:
ricelick said: Did you ever get this figured out? How did that last tub turn out?
-------------------- "If the doors of perception were cleansed every thing would appear to man as it is, infinite. For man has closed himself up, till he sees all things through narrow chinks of his cavern." - William Blake "Psychedelics helped me to escape.. albeit momentarily.. from the prison of my mind. It over-rode the habit patterns of thought and I was able to taste innocence again. Looking at sensations freshly without the conceptual overlay was very profound." - Ram Das
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Sherlock Shrooms
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Re: Please help me get this right, contamination blues.. [Re: teeter]
#27255525 - 03/16/21 11:28 AM (2 years, 10 months ago) |
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Hey everyone. I tried everything mentioned in this thread and much more over and over again and got the same poor results. And then I read a study on the effect of alkalinity on mushroom cultures, particularly contamination. The controlled study demonstrated beyond any shadow of a doubt that the substrates with a more alkaline PH had a far lower rate of contamination (interesting that they Ph'd the sub rather than a casing layer). And then I remembered that in the beginning of my adventures growing shrooms I always used a PH adjusted casing layer, the recipe straight from RR's vids. I've always tried going back to what worked in the beginning but I had forgotten and completely overlooked the casing!
So over the past few months I have been applying a casing that has been PH adjusted with oyster shell flour and hydrated lime and I have been crushing. I get 3 flushes out of every single tub now consistently over and over again. It's been glorious after 3 years of consistent failure. And what do you know, it really was spore load. I have further proof that it was spore load too, I'll share it below. It's funny because it goes against all the dogma I've heard time and time again on this site...
-------------------- "When you pass through the waters, I will be with you; And through the rivers, they shall not overflow you. When you walk through the fire, you shall not be burned, Nor shall the flame scorch you."

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Sherlock Shrooms
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Re: Please help me get this right, contamination blues.. [Re: Sherlock Shrooms]
#27255549 - 03/16/21 11:52 AM (2 years, 10 months ago) |
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Evidence number 2 is that after a month or so of consistent results I started spreading manure out in tubs to dry. I use manure, coir, verm and now also straw in my tubs. I have streamlined a pasteurization setup and I like growing in more varied nutritious subs. The shit results I had gotten over 3 years were indifferent to bucket tek coir and pasteurized manure subs, neither changed anything.
Anyways I get composted cow manure in bags and like to spread it out and dry it before mixing it up and bringing it to field capacity. I spread the manure out in tubs and could see white mycelium in different parts of it and actually thought to myself in that moment that it could become a problem regarding spore load and atomized mycelium particle load since I am drying the manure out in the same room I have tubs fruiting (that was stupid ). Then I was like naaaah it'll be fine. Sure enough about a week after I began spreading manure out in my fruiting room I started seeing contams pop up really quickly right at the beginning of the second flush in quite a few tubs. Note that at this point I had been getting 3 flushes out of all my tubs for over a month.
I quickly realized what was happening with the increased contam spore and atomized contam mycelium load in the room from the manure. So I setup a manure drying room elsewhere in the house (duh ), took all the tubs out of the fruiting room, fogged the shit out of the room with Decon 30 and then put the tubs back in it 12 hours later. And what do you know, about a week after fogging the room and removing the manure the contams slowed to a halt and I've been consistently getting 3 flushes from all of my tubs again ever since. Yet many say that spore load doesn't matter or is at least so negligible that it isn't worth considering, "it's your spawn" almost everyone chants over and over again. I mean yea I get it, all newcomers and even experienced people should always look to their sterile technique and sterilization procedures first. But the pervasive dogma and downright bullying of people who suggest there are other important variables like spore load is discouraging, a disgrace to science, and objectively untrue in my experience. It's worth noting that I haven't slipped and seen a single plate, jar, or bag show a visible contam in almost 2 years. I'm pretty sure that my sterile technique is on point at this point.
-------------------- "When you pass through the waters, I will be with you; And through the rivers, they shall not overflow you. When you walk through the fire, you shall not be burned, Nor shall the flame scorch you."

Edited by Sherlock Shrooms (03/16/21 11:55 AM)
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Sherlock Shrooms
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Re: Please help me get this right, contamination blues.. [Re: Sherlock Shrooms]
#27255559 - 03/16/21 12:03 PM (2 years, 10 months ago) |
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I also think I know why my house had a particularly high spore/mycelium load. The first year I was growing I'd carve out contams from the tubs like a fool, that didn't help. And the other factor is that I have always mixed up my substrate materials in my kitchen before pasteurizing them. Surely I was launching spores and mycelium particles everywhere in clouds of dust and over time it all started to add up. It didn't help that everywhere I looked on this site with very few illumined exceptions everyone mocked and bullied anyone who suggested spore load was a factor, then it would sometimes turn into a scholarly pissing contest. And aside from Violet, Lotkid, and a few others who stood firmly in the wisdom they forged though experience no one was pointing toward the problem I was actually having, instead they were pointing every other direction and pissing on me or others who dared consider the impact of spore load. I mean ya know, it's not like most real mushroom farms actually bomb their grow areas between each round. Oh wait they do
-------------------- "When you pass through the waters, I will be with you; And through the rivers, they shall not overflow you. When you walk through the fire, you shall not be burned, Nor shall the flame scorch you."

Edited by Sherlock Shrooms (03/16/21 12:10 PM)
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Sherlock Shrooms
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Re: Please help me get this right, contamination blues.. [Re: Sherlock Shrooms]
#27255561 - 03/16/21 12:04 PM (2 years, 10 months ago) |
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Pasty called it, thanks Pasty. I found that garbage can of thrichoderma under my steps, although my house was the garbage can That brings a kind of MC Escher image to mind 
And big thanks to everyone who chipped in and helped me find my way. Sorry I didn't write back sooner but I've been on a sort of technology fast. You guys are sharp and very generous, this is an amazing resource and community here. Much love.
-------------------- "When you pass through the waters, I will be with you; And through the rivers, they shall not overflow you. When you walk through the fire, you shall not be burned, Nor shall the flame scorch you."

Edited by Sherlock Shrooms (03/16/21 12:09 PM)
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Professor X
School for the Gifted



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Re: Please help me get this right, contamination blues.. [Re: Sherlock Shrooms]
#27255589 - 03/16/21 12:28 PM (2 years, 10 months ago) |
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I grow in a room housing 5 adult male rats and a dog with a carpeted floor. Talk about spore load. No such issues. Sounds like your casing solved your problem but tbh I think your real problem is growing cubes in archaic unnecessary crap. No benefit and all that trouble. Glad you got it ironed out.
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Blackrainbow2
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Re: Please help me get this right, contamination blues.. [Re: Professor X]
#27255654 - 03/16/21 01:40 PM (2 years, 10 months ago) |
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I was gonna say skip the Verm and use straight coir..but I'm glad you figured it out. Now tell me how you PH your sub and casing.. I need details.. Since I fucking hate green mold.. I'd love to make it's life more difficult.. ProX It's interesting you keep rats.. I had a pet rat as a kid and they are cool pets.. just like any other animal.. a friend has snakes..fun fact they are now using rats to smell out old landmines in battlefields of the world..very interesting.... now..Pro X if you could train your rats to smell clean plates and spawn..we could make some coin.. I just need 20%for my consulting fee...
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Sherlock Shrooms
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Re: Please help me get this right, contamination blues.. [Re: Blackrainbow2]
#27255709 - 03/16/21 02:26 PM (2 years, 10 months ago) |
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Professor thanks for chiping in but your assertion is unrealistic and can't possibly apply to my situation. You're literally disregarding everything I described about my trials and errors to push your predetermined point of view. You have no idea how greatly things can vary regionally not just in your environment but with raw materials available, including grains. And you have absolutely no idea how many thousands of contaminants there are in the world and how some people can get struck by lightning unlucky in the ones they unintentionally selectively breed in a growing space. I grew in straight coir for over 2 years every flipping way imaginable before going back to pasteurization. I pasteurize for fun, like I said I LIKE growing in a varied nutritious substrate. Some people enjoy what they do and don't feel the need to strip things down to the bare essentials of what works. And it does make a difference, if you haven't seen the thicker, whiter, and more vigorous mycelium of a varied nutritious substrate then you either haven't done any experimentation or aren't paying attention. Also the straw has more moisture holding capacity than coir, way more. I didn't believe it either about the spore load. But it's real, get over it. The PH casing didn't change anything except for the increased ability for my sub surface to resist airborne contaminants. And the consistent improved results that came and stayed since then coincided with no other systemic changes in what I do.
-------------------- "When you pass through the waters, I will be with you; And through the rivers, they shall not overflow you. When you walk through the fire, you shall not be burned, Nor shall the flame scorch you."

Edited by Sherlock Shrooms (03/16/21 02:33 PM)
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Sherlock Shrooms
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Re: Please help me get this right, contamination blues.. [Re: Sherlock Shrooms]
#27255725 - 03/16/21 02:37 PM (2 years, 10 months ago) |
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Sure one could say that my spawn is always bacterial and makes my sub more susceptible to airborne contaminants so ideally the casing should be unnecessary. But I've posted plenty of pics of my spawn and there are no systemic bacterial problems. I have tried many different grains, tried a wide range of PC times, tried varying degrees of dryness to the grains, etc.. and my spawn always looks the same and like it is supposed to. All my plates look super clean, flat, and symmetrical. My subs all colonize and fruit in 15 days from spawning and the sub colonizes quite evenly and consistently. No little fingers sticking up or melted ice cream look indicating bacteria. So I can't see how the PH casing could be a band-aid for bacteria rather than a realistic solution for something spore/mycelium load related.
-------------------- "When you pass through the waters, I will be with you; And through the rivers, they shall not overflow you. When you walk through the fire, you shall not be burned, Nor shall the flame scorch you."

Edited by Sherlock Shrooms (03/16/21 02:44 PM)
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Professor X
School for the Gifted



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Re: Please help me get this right, contamination blues.. [Re: Sherlock Shrooms]
#27255735 - 03/16/21 02:47 PM (2 years, 10 months ago) |
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So you say that fixing your casing solved your problem and you need this extravagant casing because you are using this extravagant substrate that absolutely does not make any difference whatsoever except to cause yourself massive headaches and hardships but that's not the issue, ninja spores from across the world are.
I have tried all sorts of crap in my substrates including crap, straw, coffee, worm castings, gypsum, and other archaic an nonsensical ingredients not needed for our species. None of it made any difference. The biggest difference was in cultures, conditions and clean spawn.
You come off as very condescending by the way. I bet you like to argue how superior shit is even though it ooked you in the dooker for years.
You also bump yourself constantly
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Sherlock Shrooms
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Re: Please help me get this right, contamination blues.. [Re: Sherlock Shrooms]
#27255744 - 03/16/21 03:00 PM (2 years, 10 months ago) |
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Blackrainbow2,I don't PH my sub I only mentioned that it is what they did in the published study. My PH casing recipe is the same as RR's from his old video series only that I use oyster shell flour instead of lime and I add 1/2 teaspoon of hydrated lime per jar of casing. It is 10 parts verm, 10 parts peat moss (pick out the large sticks), 1 part oyster shell flour (make sure it is flour and not chunks), and hydrated lime (1/2 teaspoon per jar, they say avoid high magnesium lime but I've used both a high and a low magnesium lime and can't tell a difference). I bring it to field capacity and pasteurize it in a water bath. You'll either have to let the casing sit a long time after bringing it to field capacity and check it again because the verm slowly absorbs a ridiculous amount of water, or do what I do and mix it up a specific amount over field capacity initially and put it right into the jars because I have figured out exactly how much more water the verm soaks up after while it sits and by the time I use it it's at perfect field capacity.
I do all of my sub pasteurization in bags in an extra large steamer. But the casing I do in quart jars in a water bath because I don't need that much and in jars it is easier to add to subs without cross contaminating the casing which might happen if I did a larger amount in a bag and scooped small amounts out for each tub (If one of the spawn bags for a tub had hidden contams in it..).
-------------------- "When you pass through the waters, I will be with you; And through the rivers, they shall not overflow you. When you walk through the fire, you shall not be burned, Nor shall the flame scorch you."

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Sherlock Shrooms
Neophyte



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Re: Please help me get this right, contamination blues.. [Re: Sherlock Shrooms]
#27255751 - 03/16/21 03:04 PM (2 years, 10 months ago) |
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Professor don't get your knickers in a twist. And you don't have to make shit up either. It's not an extravagant casing. It's literally the standard casing recipe people have been using for close to 20 years. Blinding leaf most recently wrote about it in noteworthy way but RR preceded him by like 10 years. And if you paid attention to my catalogued trials over the past few years you would know that I have always grown on coir and just recently started pasteurizing again. And if you would of asked I cuold explain to you that I have also grown on straight coir in my trials over the past several months alongside the pasteurized sub, NO DIFFERENCE. Can I say NO DIFFERENCE in some other way or larger letters or something to help you understand it?
-------------------- "When you pass through the waters, I will be with you; And through the rivers, they shall not overflow you. When you walk through the fire, you shall not be burned, Nor shall the flame scorch you."

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Sherlock Shrooms
Neophyte



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Re: Please help me get this right, contamination blues.. [Re: Sherlock Shrooms]
#27255755 - 03/16/21 03:10 PM (2 years, 10 months ago) |
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Professor I get along with everyone great. But you and others like you are like wind up toys that have nothing to contribute but dogmatic shit talking. It literally spoils the boards. So why bother being cordial with an ass when there is obviously no possibility of receptivity. Because of people like you I rarely ever spend any time on this site anymore.
I'm not the only one who has seen and acknowledged that nutritious substrates create slightly whiter, slightly more vigorous, and slightly quicker colonizing substrates. It's so well documented it is ridiculous stating otherwise. Now I will say it only make slight differences and unless someone would enjoy seeing that it might not be worth the effort, but the differences are there. Grow in whatever the fuck you want and I'll do the same. But when the substrate has been eliminated as variable through experimentation then let it go and discuss something more fucking relevant dude.
-------------------- "When you pass through the waters, I will be with you; And through the rivers, they shall not overflow you. When you walk through the fire, you shall not be burned, Nor shall the flame scorch you."

Edited by Sherlock Shrooms (03/16/21 03:11 PM)
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Sherlock Shrooms
Neophyte



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Re: Please help me get this right, contamination blues.. [Re: Sherlock Shrooms]
#27255760 - 03/16/21 03:14 PM (2 years, 10 months ago) |
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And no I don't argue how superior my shit is you assumption making fool. I have done nothing but struggle for years and am finally seeing consistently excellent results. I am very grateful and will remain vigilant in all my techniques. I am far from an expert. Piss off.
-------------------- "When you pass through the waters, I will be with you; And through the rivers, they shall not overflow you. When you walk through the fire, you shall not be burned, Nor shall the flame scorch you."

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Professor X
School for the Gifted



Registered: 04/18/19
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Re: Please help me get this right, contamination blues.. [Re: Sherlock Shrooms]
#27255765 - 03/16/21 03:15 PM (2 years, 10 months ago) |
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I'm good. Not going to bother reading those 3 posts you just made about me. Have a good one. Hope it all works out for you.
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