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OfflineJaredTheJeweler
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Registered: 03/26/13
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PE Agar Clone Q
    #26851717 - 07/29/20 12:27 PM (3 years, 5 months ago)



So I’ve finally gotten into agar so I can begin cloning and hopefully obtain a nice isolate. Pictured are two plates that I plan on taking samples from and moving to another plate. One is directly from the fruit body and the other is from a a cloned wbs jar. Which sectors from each look like my best bet to transfer and why?


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InvisibleZifozonke
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Registered: 03/24/19
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Re: PE Agar Clone Q [Re: JaredTheJeweler]
    #26851771 - 07/29/20 12:51 PM (3 years, 5 months ago)

Id say those are both looking rather nice already
Obtaining a pure isolate can be a long process
https://www.shroomery.org/forums/showflat.php/Number/25110917#25110917
Maybe do another one or two transfers from each and put them to grain
Kinda the only way youll see results


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OfflineA.k.aM
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Re: PE Agar Clone Q [Re: Zifozonke]
    #26851780 - 07/29/20 12:55 PM (3 years, 5 months ago)

Yeah they both look good. With this type of growth if I’m trying to narrow it down more I find the rhizo strand that’s grown furthest from the center and take a tiny bit of the tip.


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LAGM2020


Edited by A.k.a (07/29/20 12:55 PM)


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OfflineJaredTheJeweler
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Registered: 03/26/13
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Re: PE Agar Clone Q [Re: Zifozonke]
    #26852108 - 07/29/20 03:23 PM (3 years, 5 months ago)

That’s comforting to hear. Thanks for that link too, very helpful


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OfflineJaredTheJeweler
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Registered: 03/26/13
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Last seen: 7 months, 17 days
Re: PE Agar Clone Q [Re: A.k.a]
    #26852112 - 07/29/20 03:25 PM (3 years, 5 months ago)

I’ll try to let it go another day or two and then pick the furthest point as you said. I’ll try to use a wedge to make a grain jar too just to see what they would both produce right now. How many transfers could it take from here to obtain an isolate? Or is that a loaded question


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