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Offlineemzy
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Wood lover mycelium on Agar, Question Re Indoor Grow
    #26851020 - 07/29/20 01:12 AM (3 years, 5 months ago)

Hi guys,

This is my first attempt at growing psychoactive mushrooms after playing around with some oyster mushrooms. I was given a spore print from a friend in South Australia, the print was purple/ brown and definitely a psilocybin mushroom as these guys pick and consume them all the time. Im thinking the species is most likely Psilocybe Subaeruginosa or possibly Psilocybe Alutacea. They pick them in August which is the coolest months here in Australia.

See photo below of one of their recent forage



Unfortunately I did not take a photo of the spore print.

I have since colonised some agar plates With this spore print, the first batch had some contaminations, from what I have read on this site this is typical working with wild mushrooms. These had clean wedges taken to try isolate without contaminations.






I have since tried isolating from these plates and now have some clean mycelium going on new Agar plates That I think look really good and clean.




The last pic shows some contaminations carried over




The intention was to grow these out indoors on a bulk substrate in a mono tub however I just found out via the forum that wood lovers are extremely difficult to fruit indoors so kind of feel like I have wasted my time to a degree. On the up side I have had a chance to learn and practice the fundamentals I have learnt from this site such as agar, SAB, spore prints, transfers etc. this site is a true wealth of knowledge and I appreciate the community it offers

I’m going to try doing some experimenting and give it a crack as I don’t have anything else to work with yet that can be grown indoors.

I’ll also transfer some of these to a bulk substrate and try my hand at an outdoor bed as I’m thinking now even though it’s a long process I’ll probably have more success outdoors with this species but will probably take 12 months until I see results. I will post experiment progress as I go.

If anyone has any thoughts or advice I’d really appreciate the feedback. Any comments on how the last agar is looking or tips on how I could proceed are welcome or if anyone out there has cracked the Indoor Wood Lovers code I’d love to hear from you.

If anyone here in Australia that has a spore print of p.cub or similar species that is conducive to indoor growing I’d be happy to swap for one of my colonised wood lover agar plates, please PM me. I known these wood lovers are not the most sort after but someone may be looking to do an outdoor bed and maybe looking. I’m too paranoid to buy psilocybin prints online given their status in Australia even though I know many are willing to ship here from overseas.

Thanks for your help and looking forward to the comments.


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OfflineBiscuits
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Re: Wood lover mycelium on Agar, Question Re Indoor Grow [Re: emzy]
    #26851066 - 07/29/20 02:59 AM (3 years, 5 months ago)

Olive had some big success on outdoor cultivation:

https://www.shroomery.org/forums/showflat.php/Number/14553921/fpart/1/vc/1

but when they tried to move to indoor cultivation, it eventually fizzled out...:

https://www.shroomery.org/forums/showflat.php/Number/15093643/page/3/fpart/1/vc/1

I assume if you've been reading through the site then you've already stumbled on these two threads. There are a couple more but I haven't found a successful one that wasn't just a transplant moved indoors.

I've tried doing this indoors as well, and while it seems easy enough to get it to colonise woodchips (I just hydrated the brown mulch from Bunnings, no sterilisation, no pasteurisation) I could never figure out the fruiting, much like everyone else...

My advice would be to take those agar plates, throw them into some woodchips indoors under some damp cardboard or something until it colonises, then spread it all out in a huge patch of woodchips outside and keep it watered throughout the summer. You'll be thrilled that you did next winter.

One tip if you go the outdoor route: Try and have it bound by bricks or something so that you can use sand or copper to stop slugs. They were the main adversary for my private outdoor patch...

If anyone has figured out how to do it I'd be very interested as well... I have a colonized shoebox of subaeruginosa that I don't know what to do with...


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Offlineemzy
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Re: Wood lover mycelium on Agar, Question Re Indoor Grow [Re: Biscuits]
    #26851085 - 07/29/20 03:41 AM (3 years, 5 months ago)

Hey Biscuits, thanks for the reply and the tips re outdoor grow. I’ll definitely take those onboard in particular re the edging to deal with slugs.
I have read as many posts as I could find including those links re wood lovers indoors, hours and hours of reading but no one has seemed to have consistent success with decent yields from what can find anyway. Compared to p.Cubs The wood lovers seem a fruitless exercise.
Thanks again mate.


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Offlineemzy
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Re: Wood lover mycelium on Agar, Question Re Indoor Grow [Re: emzy]
    #26974900 - 10/08/20 03:59 AM (3 years, 3 months ago)

I ended up transferring my crushed up P Sub BRF Cakes to a mix of 50% ratio of Pine Bark & 50% of Hardwood Chips and the mycelium seems to real like to take to this substrate as seen in Oliver’s thread.

Did read on some other post on the forum that even though it will colonise Harwood it may not fruit on hardwood and should be Eucalyptus instead to enable fruiting, confusing as i thought that eucalyptus  was a hardwood. I did notice Oliver refers to using pine chips which is a soft wood.

Does anyone think there will be any issues with fruiting with the 50/50 mix I’m using ? Or do you fruiting should be sweet come winter given everything else is ok of course ?

I’d hate expand this to an outdoor bed with same above mix, to have this colonise all summer but not fruit come winter due to a simple error and miss another year of no fruits.

Any feedback would be appreciated, I have also included pics below of the mycelium starting to nicely colonise the wood chips and bark



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