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PiptheGreAtest
Aspiring Heavyweight


Registered: 07/18/16
Posts: 464
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Bacterial Spawn Issues
#26848856 - 07/27/20 09:24 PM (3 years, 5 months ago) |
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I have been using the NO soak No simmer tek with millet spawn and have had at least 30-50 bags come out bacterial. I used a grain/water ratio of 350g/130g and pressured cooked at 15psi for 150mins. I’m pre sealing the bags, inoculating with LC and they are fully colonized but have a sweet smell to them. Should I soak this grain or should I just switch the type of grain I’m using?
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Edited by PiptheGreAtest (07/27/20 09:34 PM)
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PiptheGreAtest
Aspiring Heavyweight


Registered: 07/18/16
Posts: 464
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Nbnlollmao
Next M Maker🙌


Registered: 07/26/20
Posts: 32
Loc: Colorful Colorado
Last seen: 3 years, 5 months
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I think you should us the PF tek wit popcorn seed easy and maybe use pint jars after all chould be the millit Yud have lsser chance to contam when jars are sturle and so is the popcorn
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Hobbit GDF
Deadhead



Registered: 02/14/19
Posts: 3,385
Loc: Terrapin station
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Re: Bacterial Spawn Issues [Re: Nbnlollmao]
#26848906 - 07/27/20 09:55 PM (3 years, 5 months ago) |
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PC your bags for 180 min then. Try that. How are you inoculating them?
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PiptheGreAtest
Aspiring Heavyweight


Registered: 07/18/16
Posts: 464
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Re: Bacterial Spawn Issues [Re: Hobbit GDF]
#26848913 - 07/27/20 10:02 PM (3 years, 5 months ago) |
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Quote:
Hobbit GDF said: PC your bags for 180 min then. Try that. How are you inoculating them?
LC I would say I had bad LC but I used 4 different cultures and I know for a fact not all them were bad.
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Smartattack
C'mon man



Registered: 12/21/18
Posts: 3,775
Loc: A thought
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Quote:
PiptheGreAtest said:
Quote:
Hobbit GDF said: PC your bags for 180 min then. Try that. How are you inoculating them?
LC I would say I had bad LC but I used 4 different cultures and I know for a fact not all them were bad.
Perhaps not, but you never know what can slip in during that inoculation window.
-------------------- * Smarts videos * Planet of the APES   I'm a fungal white supremacist.
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PiptheGreAtest
Aspiring Heavyweight


Registered: 07/18/16
Posts: 464
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I’m in front of a flowhood hopefully not too much.
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eLeSDenes
Mycelium Expander




Registered: 05/25/11
Posts: 955
Last seen: 1 year, 11 months
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Hey,
Can you show an image of your LC? Sometimes it is hard to tell if it is contaminated. I like to use just a teaspoon of extra light malt extract for 800ml of distilled water and usually cook 8-10 jars of LC for 1 hour @15psi and It comes out crystal clear which makes it much easier to tell if it is contaminated. I never use any LC if it shows the slightest cloudiness.
Bacterial contaminations are the most common thing with LC's as bacteria needs water to spread. Usually with agar to grain, there is less chance of bacteria in your spawn because bacteria on your colonised wedge cannot really spread to your dry grains. But when you work with liquids, It is very very easy to contaminate your LC when you first inoculate it, and when you use the LC for your bags, because you give the bacteria a way to multiply and spread. Mold is far less common in LC especially if you use a flowhood, but bacteria comes from you - from your breath skin and hair. So, Flowhood or not, one wrong move and you are fucked either way 
tip: if you inoculate your LC with an agar wedge, make a few fresh plates with your culture, let it grow for a few days and only use the ones which look perfect
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