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InvisiblePiptheGreAtest
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Registered: 07/18/16
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Bacterial Spawn Issues
    #26848856 - 07/27/20 09:24 PM (3 years, 5 months ago)

I have been using the NO soak No simmer tek with millet spawn and  have had at least 30-50 bags come out bacterial. I used a grain/water ratio of 350g/130g and pressured cooked at 15psi for 150mins. I’m pre sealing the bags, inoculating with LC and they are fully colonized but have a sweet smell to them. Should I soak this grain or should I just switch the type of grain I’m using?


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Edited by PiptheGreAtest (07/27/20 09:34 PM)


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InvisiblePiptheGreAtest
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Re: Bacterial Spawn Issues [Re: PiptheGreAtest]
    #26848866 - 07/27/20 09:33 PM (3 years, 5 months ago)

Here’s some examples


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OfflineNbnlollmao
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Re: Bacterial Spawn Issues [Re: PiptheGreAtest]
    #26848874 - 07/27/20 09:42 PM (3 years, 5 months ago)

I think you should us the PF tek wit popcorn seed easy and maybe use pint jars after all chould be the millit
Yud have lsser chance to contam when jars are sturle and so is the popcorn


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InvisibleHobbit GDF
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Re: Bacterial Spawn Issues [Re: Nbnlollmao]
    #26848906 - 07/27/20 09:55 PM (3 years, 5 months ago)

PC your bags for 180 min then. Try that. How are you inoculating them?


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InvisiblePiptheGreAtest
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Re: Bacterial Spawn Issues [Re: Hobbit GDF]
    #26848913 - 07/27/20 10:02 PM (3 years, 5 months ago)

Quote:

Hobbit GDF said:
PC your bags for 180 min then. Try that. How are you inoculating them?



LC I would say I had bad LC but I used 4 different cultures and I know for a fact not all them were bad.


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InvisibleSmartattack
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Re: Bacterial Spawn Issues [Re: PiptheGreAtest]
    #26848948 - 07/27/20 10:44 PM (3 years, 5 months ago)

Quote:

PiptheGreAtest said:
Quote:

Hobbit GDF said:
PC your bags for 180 min then. Try that. How are you inoculating them?



LC I would say I had bad LC but I used 4 different cultures and I know for a fact not all them were bad.





Perhaps not, but you never know what can slip in during that inoculation window.


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InvisiblePiptheGreAtest
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Re: Bacterial Spawn Issues [Re: Smartattack]
    #26848967 - 07/27/20 10:54 PM (3 years, 5 months ago)

I’m in front of a flowhood hopefully not too much.


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OfflineeLeSDenes
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Re: Bacterial Spawn Issues [Re: PiptheGreAtest]
    #26876743 - 08/12/20 11:27 AM (3 years, 5 months ago)

Hey,

Can you show an image of your LC? Sometimes it is hard to tell if it is contaminated. I like to use just a teaspoon of extra light malt extract for 800ml of distilled water and usually cook 8-10 jars of LC for 1 hour @15psi and It comes out crystal clear which makes it much easier to tell if it is contaminated. I never use any LC if it shows the slightest cloudiness.

Bacterial contaminations are the most common thing with LC's as bacteria needs water to spread. Usually with agar to grain, there is less chance of bacteria in your spawn because bacteria on your colonised wedge cannot really spread to your dry grains. But when you work with liquids, It is very very easy to contaminate your LC when you first inoculate it, and when you use the LC for your bags, because you give the bacteria a way to multiply and spread.
Mold is far less common in LC especially if you use a flowhood, but bacteria comes from you - from your breath skin and hair. So, Flowhood or not, one wrong move and you are fucked either way :laugh:

tip: if you inoculate your LC with an agar wedge, make a  few fresh plates with your culture, let it grow for a few days and only use the ones which look perfect


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