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OfflineC12ShroomMan
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Registered: 01/19/20
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How long can i keep making transfers of transfers (agar)
    #26848724 - 07/27/20 08:09 PM (3 years, 5 months ago)

Lets say every time i drop agar to grain i take a cutting to start a new plate, can i continue this cycle infinitely?


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Offlineskullhuman
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Registered: 06/15/09
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Re: How long can i keep making transfers of transfers (agar) [Re: C12ShroomMan]
    #26848777 - 07/27/20 08:34 PM (3 years, 5 months ago)

I can't quantify it with an exact number, but you can get away with quite a few agar transfers because not too many cell divisions happen on plates.  Senescence sets in after too many cell divisions, and obviously many more cell divisions are taking place throughout, an entire bulk grow for example, vs plate transfers.


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OfflineShroomer J
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Registered: 06/27/18
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Re: How long can i keep making transfers of transfers (agar) [Re: skullhuman]
    #26848791 - 07/27/20 08:44 PM (3 years, 5 months ago)

As long as you are contamination free, keep going until the growth slows. Years back, I got at least 25 generations of the B+ strain. Don't do agar any more, just grain to grain. Keep it clean and keep going.


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