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OfflineIgnorantOne
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Need Guidance
    #26847911 - 07/27/20 01:12 PM (3 years, 6 months ago)

Apologies ahead of time if the post is scatterbrained. Coffee makes the brain process a little too quickly.

I could use some guidance. I recently made the jump to a PC, Agar, and WBS. For hydrating the WBS I have done the Foo Man tek and soaking overnight. Once jars are loaded they get PC'd for 60-90 minutes. Once cooled they are then shaken and inoculated with agar wedges. My agar is made using Pasty Whyte's Easy Agar Tek.

Out of 6-7 bins I have only managed to get one to blob/knot, all of them have gone the way of my WTF Bin which is posted in the photos. My suspicion is that my bins are too wet. Additionally, I have had some jars stall out and, upon emptying, they smell fermented and sting the sinuses. So overall, I think my moisture content is an issue and causing bacteria issues. I have somewhat rectified this with my jars by drying the WBS longer. Additionally, I have added some verm to a few jars to handle excess moisture (they colonize much faster than others now). The sub spawned to is Coir/Verm 50/50 soaked in a cooler with boiling water for an hour. I squeeze the hot substrate till it only drips under pressure and mix it 1:1 with spawn which then is given a top layer.

Ok, so my bins colonize quickly but seem to build up moisture too. They are shoeboxes that were umodded until recently. I have added some vent holes covered with two layers of micropore tape (what I have atm) to aid with the moisture.  Am I on the right track here? Is there some important item I am missing? I can get brf cakes to fruit, can get brf cakes spawned to coir to fruit, but cannot get wbs to fruit..

Photos Below.. the wtf bin is embarrassing.



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OfflineSFS96
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Re: Need Guidance [Re: IgnorantOne]
    #26847934 - 07/27/20 01:22 PM (3 years, 6 months ago)

Iโ€™m seeing a lot of bursted grains In those jars. Iโ€™d modify the grain prep that your using. What Tek do u use that has all those busting grains? The fooman Tek? Iv never had that many burst grains with the 24 hr soak method.


Edited by SFS96 (07/27/20 01:23 PM)


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Offlinehazyhorse
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Re: Need Guidance [Re: IgnorantOne]
    #26847940 - 07/27/20 01:23 PM (3 years, 6 months ago)

hard for me to tell from pics but that WTF tub is definitely fucked. idk if bacteria is going on there or what but it does not look normal. looks like overlay or something. i think your grains being too wet was a major factor. i use horse oats, but when my grains were too wet i ran into a bunch of problems like bacteria & slow colonization. iโ€™ve never used WBS but in general spawn is better on the drier side. i just use bods oat prep tek & when i make them on the dry side they colonize great. if you can spawn cakes to bulk & get results, i would say the WBS is probably the issue youโ€™re running into. making sure you use properly hydrated grains & clean agar plates should set you up for success if you can grow bulk BRF!!


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OfflineIgnorantOne
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Re: Need Guidance [Re: hazyhorse]
    #26847968 - 07/27/20 01:37 PM (3 years, 6 months ago)

The burst grains came from my dumbass forgetting the heat was on while attempting Foo Man's tek. Could not figure out how to filter them out so I just rolled with it. The soaking method I started doing recently and the burst grain issue has stopped.

How long do people typically dry their grains? Should I stick to the toilet paper test to ensure dryness?

I feel like I know what I am doing wrong but need it pointed out to me for some reason.


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Ignorance is bliss when tis folly to be wise.



Agar Sectoring/Isolation
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Edited by IgnorantOne (07/27/20 01:37 PM)


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OfflineSFS96
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Re: Need Guidance [Re: IgnorantOne]
    #26847994 - 07/27/20 01:47 PM (3 years, 6 months ago)

Most people load up WBS kind of wet. I like to let my mine strain for a couple of hrs before loading but they still seem pretty wet. The number one thing for a successful monotub is clean spawn. So if you get your grain on point and your still having issues then itโ€™s probably your agar work. Also 50/50 is not the best ratio for coir and verm.  If you follow  the bucket Tek you use a brick of coir and 2 qts of vermiculite which comes out to about 80% coir and 20% verm.


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Offlinehazyhorse
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Re: Need Guidance [Re: IgnorantOne]
    #26848009 - 07/27/20 01:52 PM (3 years, 6 months ago)

Quote:

IgnorantOne said:
I feel like I know what I am doing wrong but need it pointed out to me for some reason.




this quote is so applicable to life in general lol. such a weird human thing

i know oats are probably different than WBS, but i let mine dry for a few hours or overnight in the fridge


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you're not the first to set foot here, just another
===================================
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posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

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OfflineIgnorantOne
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Re: Need Guidance [Re: hazyhorse]
    #26848176 - 07/27/20 03:11 PM (3 years, 6 months ago)

Ok. I will start drying for longer. Have either of you tried adding verm to your grain jar? I did that with one set of them and they colonized the fastest.

Oh, the agar I am using to inoculate is in the pictures. Been only using clean sections of it, cutting wedges out with a flame sterilized exacto knife. The ones pictured above are... fuck is there a term for "clean up" plates? They were made with clean wedges from plates I made using spores.


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Ignorance is bliss when tis folly to be wise.



Agar Sectoring/Isolation
Mono
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Edited by IgnorantOne (07/27/20 03:11 PM)


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OfflineSFS96
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Re: Need Guidance [Re: IgnorantOne]
    #26848240 - 07/27/20 03:43 PM (3 years, 6 months ago)

WBS doesnโ€™t need to be dry as other grains but 2hrs of dry time works good for me but many people only wait 20 or 30 for wbs. No Iv never added vermiculite to jars. That doesnโ€™t make them hard to break up? Also I wouldnโ€™t use plates like that to inoculate, you need to make more transfers until you get a nice even culture then go to grain. Your plates still have some contamination from what I can see and need to be cleaned up more.


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InvisibleLadysKnight
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Re: Need Guidance [Re: SFS96]
    #26848259 - 07/27/20 03:52 PM (3 years, 6 months ago)

You should take a closer look at the Fooman Tek. There is no soaking. This is why your wbs is too wet.
But yeah, there's mold on that plate.


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OfflineSFS96
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Re: Need Guidance [Re: LadysKnight]
    #26848275 - 07/27/20 03:58 PM (3 years, 6 months ago)

I thinks heโ€™s talking about 2 different teks in the OP. He said heโ€™s tried foo mans Tek and overnight soaking


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OfflineIgnorantOne
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Re: Need Guidance [Re: SFS96]
    #26848282 - 07/27/20 04:04 PM (3 years, 6 months ago)

Quote:

LadysKnight said:
You should take a closer look at the Fooman Tek. There is no soaking. This is why your wbs is too wet.
But yeah, there's mold on that plate.




Which agar plate has mold on it?

Quote:

SFS96 said:
I thinks heโ€™s talking about 2 different teks in the OP. He said heโ€™s tried foo mans Tek and overnight soaking




You are correct. I meant that I had tried both methods, not that I did Foo Man's method and then soaked.


--------------------
Ignorance is bliss when tis folly to be wise.



Agar Sectoring/Isolation
Mono
How to PC
Improved PF Tek


Edited by IgnorantOne (07/30/20 08:11 PM)


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OfflineSFS96
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Re: Need Guidance [Re: IgnorantOne]
    #26848299 - 07/27/20 04:14 PM (3 years, 6 months ago)

The first plate you posted has some kind of contamination Iโ€™m not sure if itโ€™s actually mold but itโ€™s some kind of contamination right around 3 or 4 oโ€™clock. The second plate has something going on too at around 11 or 12 oโ€™clock also. You want even growth all the way across your plates.


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How I make and preserve tea


Consuming consumes a man That was never a purpose of life To only crave for material joys Is believing the lie - Mellow Mood


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OfflineIgnorantOne
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Re: Need Guidance [Re: SFS96]
    #26848333 - 07/27/20 04:31 PM (3 years, 6 months ago)

Quote:

SFS96 said:
The first plate you posted has some kind of contamination Iโ€™m not sure if itโ€™s actually mold but itโ€™s some kind of contamination right around 3 or 4 oโ€™clock. The second plate has something going on too at around 11 or 12 oโ€™clock also. You want even growth all the way across your plates.




These two spots?

I have been attempting to only take sections of agar with the rhizomorphic growth. That reminds me, are there any molds that are identical to cubensis myc in look and smell?



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Ignorance is bliss when tis folly to be wise.



Agar Sectoring/Isolation
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OfflineBroken Veils
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Re: Need Guidance [Re: IgnorantOne]
    #26848621 - 07/27/20 07:14 PM (3 years, 6 months ago)

Yes - any spots or sections that create an abnormal rift in growth are worth a second look.  As for molds that are identical to myc in look and smell...there are a few that can start out "looking" like myc but soon reveal their true form.  You should not be smelling agar cultures :wink: but some contams can hide beneath the earthy mushroom smell in grain jars


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OfflineSFS96
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Re: Need Guidance [Re: IgnorantOne]
    #26849016 - 07/27/20 11:29 PM (3 years, 6 months ago)

Quote:

IgnorantOne said:
Quote:

SFS96 said:
The first plate you posted has some kind of contamination Iโ€™m not sure if itโ€™s actually mold but itโ€™s some kind of contamination right around 3 or 4 oโ€™clock. The second plate has something going on too at around 11 or 12 oโ€™clock also. You want even growth all the way across your plates.




These two spots?

I have been attempting to only take sections of agar with the rhizomorphic growth. That reminds me, are there any molds that are identical to cubensis myc in look and smell?






Yeah those are the spots. You should transfer from the good area and see how it goes. Once you have a even plate then go to grain.


--------------------
How I make and preserve tea


Consuming consumes a man That was never a purpose of life To only crave for material joys Is believing the lie - Mellow Mood


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OfflineIgnorantOne
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Re: Need Guidance [Re: SFS96]
    #26849765 - 07/28/20 11:50 AM (3 years, 6 months ago)

Quote:

Broken Veils said:
Yes - any spots or sections that create an abnormal rift in growth are worth a second look.  As for molds that are identical to myc in look and smell...there are a few that can start out "looking" like myc but soon reveal their true form.  You should not be smelling agar cultures :wink: but some contams can hide beneath the earthy mushroom smell in grain jars



Quote:

SFS96 said:
Quote:

IgnorantOne said:
Quote:

SFS96 said:
The first plate you posted has some kind of contamination Iโ€™m not sure if itโ€™s actually mold but itโ€™s some kind of contamination right around 3 or 4 oโ€™clock. The second plate has something going on too at around 11 or 12 oโ€™clock also. You want even growth all the way across your plates.





these two spots?

I have been attempting to only take sections of agar with the rhizomorphic growth. That reminds me, are there any molds that are identical to cubensis myc in look and smell?






Yeah those are the spots. You should transfer from the good area and see how it goes. Once you have a even plate then go to grain.




Ok, I will apply this information. Guess it is time to make some new plates lol. Thank you for your help, will update when updates happen.


--------------------
Ignorance is bliss when tis folly to be wise.



Agar Sectoring/Isolation
Mono
How to PC
Improved PF Tek


Edited by IgnorantOne (07/28/20 11:51 AM)


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OfflineIgnorantOne
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Re: Need Guidance [Re: IgnorantOne]
    #26860327 - 08/03/20 09:08 AM (3 years, 6 months ago)

Update:

Pictured below are a couple of bins that were looking good until all these blobs appeared yesterday. IME they do not look like knots and I have no idea what they are. I assume contamination as they were made using jars inoculated from my assumed clean agar plates and not the new ones I am making upon y'alls advice. Any idea what these damn blobs are?



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Ignorance is bliss when tis folly to be wise.



Agar Sectoring/Isolation
Mono
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Offlinehazyhorse
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Re: Need Guidance [Re: IgnorantOne]
    #26860912 - 08/03/20 01:42 PM (3 years, 6 months ago)

it still looks like overlay or something to me. idk i donโ€™t think those tubs look healthy. what strain are you cultivating? PE is prone to blobbing, blobs can happen with any mushrooms i believe but PE tends to more often


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===================================

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OfflineIgnorantOne
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Re: Need Guidance [Re: hazyhorse]
    #26860918 - 08/03/20 01:45 PM (3 years, 6 months ago)

Umm, I actually forgot to label the jars.. So it is one of three: Malabar, B+, or HP. I vaguely remember it being my Malabars.


I was looking up "weird blobs" and came across Mycogone as a possible culprit but the blobs are not leaking yellow and the sub is not overly wet. The photos are of two tubs, made at different times, both with those blobs. A deep part of me secretly hopes these are blobs that will become pins. I have learned not to trust that wishfulness when it comes to this hobby.

All else fails I am going to start making clone plates instead of trying to germinate spores on agar.


--------------------
Ignorance is bliss when tis folly to be wise.



Agar Sectoring/Isolation
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Edited by IgnorantOne (08/03/20 02:14 PM)


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Offlinew00tmycelium
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Re: Need Guidance [Re: IgnorantOne]
    #26877100 - 08/12/20 03:50 PM (3 years, 6 months ago)

Those all look perfectly fine to me, just give them some light and air and keep water levels on point.

EDIT:  I just saw your updates, those tubs don't look healthy overall, how is the smell?


Edited by w00tmycelium (08/12/20 03:51 PM)


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