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OfflineSherlock Shrooms
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Registered: 06/25/19
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Agar - sectors not touching each other or contamination?
    #26847743 - 07/27/20 11:46 AM (3 years, 5 months ago)

Hey guys. These are texan multispore 4 transfers away from spore. I've never seen anything like this. The sectors look like they're not touching each other with gaps of uncolonized agar between them. Is this normal behavior or is there some hidden contamination causing this? In the second pic you can see where the gap formed and as the culture grew out it closed in around it. Strange.. Not sure if this is safe to drop or if I should keep tranferring. Aside from the gaps it looks healthy..




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"When you pass through the waters, I will be with you; And through the rivers, they shall not overflow you. When you walk through the fire, you shall not be burned, Nor shall the flame scorch you."


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OfflineKuroNeko
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Re: Agar - sectors not touching each other or contamination? [Re: Sherlock Shrooms]
    #26847747 - 07/27/20 11:49 AM (3 years, 5 months ago)

Bottom pictures would be better. Please post those.


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OfflineOne of Us
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Re: Agar - sectors not touching each other or contamination? [Re: KuroNeko]
    #26847776 - 07/27/20 12:10 PM (3 years, 5 months ago)

Gaps are not a good sign. Keep transferring


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OfflineSherlock Shrooms
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Re: Agar - sectors not touching each other or contamination? [Re: KuroNeko]
    #26847899 - 07/27/20 01:08 PM (3 years, 5 months ago)

Ok will keep transferring, thank you!


--------------------
"When you pass through the waters, I will be with you; And through the rivers, they shall not overflow you. When you walk through the fire, you shall not be burned, Nor shall the flame scorch you."


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OfflineKuroNeko
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Re: Agar - sectors not touching each other or contamination? [Re: One of Us]
    #26847904 - 07/27/20 01:10 PM (3 years, 5 months ago)

Quote:

One of Us said:
Gaps are not a good sign. Keep transferring




I have only seen myc split due to meeting with bacterial spot. Sometimes it's not visible but if area of myc around it is yellowing from antibiotic secretion then sure you got a contam there.


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OfflineSherlock Shrooms
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Re: Agar - sectors not touching each other or contamination? [Re: KuroNeko]
    #26847913 - 07/27/20 01:13 PM (3 years, 5 months ago)

Yea there's no yellowing. It looks like totally healthy myc that is just splitting. And would that be bacteria already present on the agar rather than something in the culture? It seems like it grows out totally healthy and fine then suddenly splits around a given area..


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"When you pass through the waters, I will be with you; And through the rivers, they shall not overflow you. When you walk through the fire, you shall not be burned, Nor shall the flame scorch you."


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OfflineKuroNeko
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Re: Agar - sectors not touching each other or contamination? [Re: Sherlock Shrooms]
    #26847957 - 07/27/20 01:32 PM (3 years, 5 months ago)

If there is no signs at all on bottom side then someone with more plates under belt needs to tell what's going on. Maybe some rare genetic trait? I've browsed most agar threads when learning and don't remember anyone talking about this. Would like to know too.


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OfflineSherlock Shrooms
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Re: Agar - sectors not touching each other or contamination? [Re: KuroNeko]
    #26848157 - 07/27/20 03:00 PM (3 years, 5 months ago)

There's no signs on the bottom. It looks super clean really aside from gaps between the sectors. You can even see fine myc threads reaching over the gap and touching each other. Strange. Like you I've never seen this before. Oh and check this out, these are the 4th transfer away from spore. The 2 and 3rd transfers weren't doing this gap thing at all. And multiple different plates on the 4th transfer are doing it and some aren't...

I'll have my girlfriend take illuminated pics later from the front and back so you can see the gaps and myc threads very clearly. Her camera is fancy and I don't know how to use it :lol:


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"When you pass through the waters, I will be with you; And through the rivers, they shall not overflow you. When you walk through the fire, you shall not be burned, Nor shall the flame scorch you."


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