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Offlinesonoramo
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What to do with a mold-killer?
    #26847727 - 07/27/20 11:31 AM (3 years, 5 months ago)

I'd like to hear opinions about whether trying to isolate a strain that overgrows mold is worth the effort. This is a second transfer I made a week ago, to isolate strains from a MS germination plate. I inoculated the "original" plate from a friend's Golden Teacher spore print. I neglected those plates for 2 months, and three of them actually fruited from the agar :ashamed: Finally got around to transferring onto fresh agar last week, and the culture in the picture is the fastest grower of the lot. You can see the bit of mushroom I transferred in the middle. It's also one that got contaminated by mold.

What I've never seen before is mycelium growing right over the mold! This is a characteristic I'd really like to have in my grain jars. So I'm ready to try another transfer from the edge farthest from the moldy spots.

Has anyone else seen mycelium seem to defeat mold? Suggestions how to proceed?

Thanks!


The mold killer?


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Offlinerido
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Re: What to do with a mold-killer? [Re: sonoramo]
    #26847740 - 07/27/20 11:44 AM (3 years, 5 months ago)

Transferring opposite the mold would usually be preferred, but there is plenty of sectoring on that plate which means it is not an isolated culture. If you want to keep the potential mold-killing phenotype, transfer from the leading edge of the mold-killing sector. Yes, you will bring mold with the transfer, oh well.

You might also consider hanging onto that culture so you can eventually make a "face off" style plate: a mold transfer and a myc transfer, each with equal space between the plate edge and the other transfer. This will let you more directly observe the myc/mold interaction. Does the myc inhibit the mold? Or is it just coming along for trich's sloppy seconds? What other phenotypes are along for the ride?


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Offlinesonoramo
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Re: What to do with a mold-killer? [Re: rido]
    #26848041 - 07/27/20 02:02 PM (3 years, 5 months ago)

Quote:

rido said:
Transferring opposite the mold would usually be preferred, but there is plenty of sectoring on that plate which means it is not an isolated culture. If you want to keep the potential mold-killing phenotype, transfer from the leading edge of the mold-killing sector. Yes, you will bring mold with the transfer, oh well.

You might also consider hanging onto that culture so you can eventually make a "face off" style plate: a mold transfer and a myc transfer, each with equal space between the plate edge and the other transfer. This will let you more directly observe the myc/mold interaction. Does the myc inhibit the mold? Or is it just coming along for trich's sloppy seconds? What other phenotypes are along for the ride?




Thank you!  All great ideas. Also speaks to the value of having plenty of clean agar plates ready for experimentation fun!


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OfflineSFS96
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Re: What to do with a mold-killer? [Re: sonoramo]
    #26848251 - 07/27/20 03:49 PM (3 years, 5 months ago)

If you transfer from the “mold killer” area your just going to end up growing a bunch of mold. Just because it’s growing over the mold doesn’t mean it’s killing it... The mold will return.


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Consuming consumes a man That was never a purpose of life To only crave for material joys Is believing the lie - Mellow Mood


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InvisiblebodhisattaMDiscordReddit
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Re: What to do with a mold-killer? [Re: SFS96]
    #26848291 - 07/27/20 04:08 PM (3 years, 5 months ago)

People sometimes think their mycelium won against contamination. Like a person with aids won against hiv because they manage to coexist together. You don't want moldy mycelium.


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