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OfflineYeetusdeetus
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Variables that speed up lc
    #26846876 - 07/26/20 09:01 PM (3 years, 6 months ago)

Thinking of getting a stir plate but I’ve heard conflicting opinions on whether it significantly speeds up lc colonization. I’ve followed mushboy’s lc tek which doesn’t provide any ge but seems to work fine as long as the broth doesn’t fill more than half the bottle.

Has anybody compared lc with a stir plate to one that’s only stirred once every day or two? Or compared lc with ge to no ge?

I know individual cultures will perform differently under different circumstances but I’d like to hear about any experience anecdotal or otherwise


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OfflineCagedApe
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Re: Variables that speed up lc [Re: Yeetusdeetus]
    #26847015 - 07/27/20 12:13 AM (3 years, 6 months ago)

Warmth. A kombucha warming plate comes to mind.


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OfflineSFS96
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Re: Variables that speed up lc [Re: CagedApe]
    #26847019 - 07/27/20 12:19 AM (3 years, 6 months ago)

I don’t know if a stir plate makes it faster or not but it keeps the LC from clumping up which I like:thumbup:


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Invisiblec10h12n2o
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Re: Variables that speed up lc [Re: SFS96]
    #26847071 - 07/27/20 02:24 AM (3 years, 6 months ago)

I love my stir plate

Definitely speeds things up

But lc is plenty fast either way


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"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
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OfflineNbnlollmao
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Re: Variables that speed up lc [Re: c10h12n2o]
    #26847075 - 07/27/20 02:28 AM (3 years, 6 months ago)

Can you help me with sum questions on mushroom injection and collinizing ?


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Invisiblec10h12n2o
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Re: Variables that speed up lc [Re: Nbnlollmao]
    #26847081 - 07/27/20 02:42 AM (3 years, 6 months ago)

What exactly are your questions?


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"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
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InvisiblebodhisattaMDiscordReddit
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Re: Variables that speed up lc [Re: Nbnlollmao]
    #26847142 - 07/27/20 04:38 AM (3 years, 6 months ago)

Quote:

Nbnlollmao said:
Can you help me with sum questions on mushroom injection and collinizing ?



Yes colonizing is how you spell it.
Collin-izing isn't a mushroom thing.


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OfflineYeetusdeetus
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Re: Variables that speed up lc [Re: c10h12n2o]
    #26847185 - 07/27/20 05:28 AM (3 years, 6 months ago)

Quote:

c10h12n2o said:
I love my stir plate

Definitely speeds things up

But lc is plenty fast either way




How much broth do you usually make and how much faster would you say it is compared to manually stirred lc? I only plan on doing less than 400ml per bottle so I’m not sure how comparable our methods would be.

I mainly wanna get a stir plate so I can try out some of those vented PTFE media bottle caps. I’m clumsy af and I don’t wanna get em wet if I can help it.


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OfflineYeetusdeetus
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Re: Variables that speed up lc [Re: SFS96]
    #26847187 - 07/27/20 05:30 AM (3 years, 6 months ago)

Quote:

SFS96 said:
I don’t know if a stir plate makes it faster or not but it keeps the LC from clumping up which I like:thumbup:




I’ve just been swirling the bottle with broken glass in it and it’s been keeping clumps out well enough lol.


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OfflineA.k.aM
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Re: Variables that speed up lc [Re: Yeetusdeetus]
    #26847195 - 07/27/20 05:42 AM (3 years, 6 months ago)

I don’t even put anything in mine, just 250ml in a media bottle. I’ll swirl it really fast and then suddenly switch directions and it seems to make the myc blobs explode. I’ve used them at five days old many times and gotten week or less colonization.


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OfflineYeetusdeetus
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Re: Variables that speed up lc [Re: A.k.a]
    #26847201 - 07/27/20 05:47 AM (3 years, 6 months ago)

How often do you swirl?


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OfflineA.k.aM
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Re: Variables that speed up lc [Re: Yeetusdeetus]
    #26847212 - 07/27/20 05:59 AM (3 years, 6 months ago)

At least once a day. Sometimes up to 5 times depending how often I’m in the room.

Having adequate sugars in the broth seems to make more of a difference ime. I started out making really light mixes based on some recipes people posted and it would start out fine but never get super thick colonization like I’d see a lot of pictures of. Once I jacked up the lme growth didnt stall out anymore.

I think last time for 250ml I used 2g lme and a drop of Karo just cuz I have a huge bottle.


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OfflineYeetusdeetus
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Re: Variables that speed up lc [Re: A.k.a]
    #26847218 - 07/27/20 06:13 AM (3 years, 6 months ago)

Oh shit I might try using more lme in my broth next time. Last time I used 0.4% for 250 ml which is what mushboy recommends. I’m just worried about upping the nutes because I’ve read that higher nute lc tends to be more O2 hungry, don’t wanna suffocate it lol. Might just order a couple of those caps tomorrow anyways since they’re only like 5 bucks.


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OfflineA.k.aM
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Re: Variables that speed up lc [Re: Yeetusdeetus]
    #26847268 - 07/27/20 07:18 AM (3 years, 6 months ago)

2g might be too much, but I started out real low like you said too and for whatever reason had better results with more.

I use my media bottle so it’s got plenty of air in there. Also the cap let’s gas exchange happen but I usually wrap it with cling too.


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OfflineYeetusdeetus
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Re: Variables that speed up lc [Re: A.k.a]
    #26847289 - 07/27/20 07:42 AM (3 years, 6 months ago)

Man I’d never thought about wrapping the lid. I’ll probably try it out with some para later today and see how it does. Thanks for the advice dude  :rockon:


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Invisiblemaxmush
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Re: Variables that speed up lc [Re: Yeetusdeetus]
    #26847503 - 07/27/20 09:46 AM (3 years, 6 months ago)

Use a simple 4% karo solution and leave it for at least 2-3 days and then spin a few times a day. You'll be surprised how fast it grows. I have seen posts where they did back to back tests using a magnetic stirrer immediately and on all day. These apparently grew faster, but its is my opinion to only disrupt the myc before use is best. I also found that no to minimal GE helps speed it up at the beginning. Just my 2 cents


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Edited by maxmush (07/27/20 09:48 AM)


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OfflineYeetusdeetus
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Re: Variables that speed up lc [Re: maxmush]
    #26847892 - 07/27/20 01:04 PM (3 years, 6 months ago)

I was under the impression that Karo was a poor nute source compared to me and gw lc due to the simple nutritional makeup.


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Invisiblec10h12n2o
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Re: Variables that speed up lc [Re: Yeetusdeetus]
    #26848004 - 07/27/20 01:50 PM (3 years, 6 months ago)

You dont need much nutrition at all in the lc. Doesnt really matter tho

I use less than 0.17% sugars in my lc. 1l water to 1.67g lme

Stir plate isnt just for clumps, it makes it grow faster by increasing myc surface and exposing to more liquid

It definitely isnt essential tho. I make several vessels between a pint and a quart at a time (per vessel),  and can use it after about 4 to 6 days on the plate, or 7 - 10 days without


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"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche


Edited by c10h12n2o (07/27/20 03:24 PM)


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InvisiblemushboyMDiscord
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Re: Variables that speed up lc [Re: c10h12n2o]
    #26848054 - 07/27/20 02:08 PM (3 years, 6 months ago)

:whathesaid:

I also find that a healthy culture is what colonizes lc nice and fast.

Imo variables that slow lc down are contams, too much nutrition, shitty cultures.


Edited by mushboy (07/27/20 02:09 PM)


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Invisiblec10h12n2o
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Re: Variables that speed up lc [Re: mushboy]
    #26848209 - 07/27/20 03:26 PM (3 years, 6 months ago)

yeah a good clean culture makes all the difference in the world.

What mushboy said is spot on.  to the extent that if it doesnt colonize fairly fast i dont even work with it


--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche


Edited by c10h12n2o (07/27/20 03:27 PM)


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