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Fastguy199
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CONTAM??? Help!! I’m an amateur!
#26845330 - 07/25/20 11:16 PM (3 years, 6 months ago) |
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Possible contams? What do y’all think and what should I do?
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Fastguy199
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Re: CONTAM??? Help!! I’m an amateur! [Re: Fastguy199]
#26845340 - 07/25/20 11:25 PM (3 years, 6 months ago) |
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Bought the agar dishes from amazon, pre sterilized. inoculated with APES syringe and now a few days later getting gold-brown around the actual spore sample itself in the dish.. help!! (The spore samples within the dish were rather large.) is this a contam? Or oxidation?
Edited by Fastguy199 (07/25/20 11:31 PM)
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Goatrider
Rhythm Guitarist



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Re: CONTAM??? Help!! I’m an amateur! [Re: Fastguy199]
#26845416 - 07/26/20 01:43 AM (3 years, 6 months ago) |
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First...
You`ve chosen one of the worst backgrounds for agar pictures i`ve ever seen
How much spores you shot on the plate? One drop would be enough. After a few days you won`t have much myc growth from syringe, so you have contams in there.
Inoculated in SAB? Flame sterilized the needle?
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c10h12n2o
serial dilutor



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Re: CONTAM??? Help!! I’m an amateur! [Re: Goatrider]
#26845418 - 07/26/20 01:47 AM (3 years, 6 months ago) |
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Quote:
Goatrider said: First...
You`ve chosen one of the worst backgrounds for agar pictures i`ve ever seen
Probably the worst lol.. like might be a prank
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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Mr.Christmas
Who?



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Re: CONTAM??? Help!! I’m an amateur! [Re: c10h12n2o]
#26845438 - 07/26/20 02:23 AM (3 years, 6 months ago) |
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I, for one, love your counter. Is it fake marble? That looks like contamination, cut it out.
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Todo lo que hagas en la vida será insignificante; pero es muy importante que lo hagas.
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Biscuits



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Re: CONTAM??? Help!! I’m an amateur! [Re: Mr.Christmas]
#26845509 - 07/26/20 04:48 AM (3 years, 6 months ago) |
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The counter looks nice however it's the same colour as the myc and contams, which I think was his problem with it lol...
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Fastguy199
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Re: CONTAM??? Help!! I’m an amateur! [Re: Biscuits]
#26845677 - 07/26/20 08:18 AM (3 years, 6 months ago) |
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The mycelium is growing (shooting) outwards from my spores.. there are quite a few spores in there, this was my first go at agar! Sorry about the counter top, if a lot of you had brains you’d click on the image to be able to view it. Didn’t realize a countertop was that big a deal.. I also labeled the pictures and took very clean clear pictures so everybody could see what’s going on.
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Goatrider
Rhythm Guitarist



Registered: 04/08/20
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Re: CONTAM??? Help!! I’m an amateur! [Re: Fastguy199]
#26845703 - 07/26/20 08:39 AM (3 years, 6 months ago) |
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Ah, one can click on the image? Shit, if i had brains i`d have known this before.
Your reaction isn`t the best way to get any further answers, that you already got, by the way.
If you had brains, you`d have read them.
I`m off here now.
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Biscuits



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Re: CONTAM??? Help!! I’m an amateur! [Re: Goatrider]
#26845766 - 07/26/20 09:31 AM (3 years, 6 months ago) |
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Quote:
Fastguy199 said: Sorry about the counter top, if a lot of you had brains you’d click on the image to be able to view it.
enlarging the image changes the counter top colour? 
Yes those spots are contams... Try and take a transfer of some healthy mycelium to new plates soon if the contams continue to grow so aggressively... Maybe if you "had brains" you'd be able to figure that out with a simple search...
Also not sure what is cube myc and what is mould. a lot of those germination sites have weird, not-white colours in the centre...
Good luck on your grow... Jeez
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Fastguy199
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Re: CONTAM??? Help!! I’m an amateur! [Re: Biscuits]
#26846048 - 07/26/20 12:31 PM (3 years, 6 months ago) |
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I was only being a dick to the people talking about my countertops. You can obviously see the myc and agar dish just fine— anyways!
Yes there are black spots. Those are spores that came out of the syringe.. I shook the hell out of it but there were some pretty big pieces still. I haven’t been able to find any other contam like this(in any of my notes,books, or online) I have 8 other dishes and did them all the same way( No sab No flow hood) I cleaned my counters and removed everything, turned fans and ac off for a good 45 mins and sprayed the hell out of the air and all suppounring walls and counters with 70% ipa) I have done bags and brf cakes this way with no issues and like I said I have 8 other dishes done on the same day(all different strains from different companies) and all of them were successful.(have healthy myc) (and some are pans)I will post pics if y’all would like?
Flame steril needle, and 70% ipa on EVERYTHING before I use it. Also wash hands multiple times with soap and the ipa. I’m super clean when I do this! I know it could be better but like I’ve said. This is the only dish I am “worried” about. And it could also be because it’s APES I’ve read they are hard to get spores from cleanly???
Edited by Fastguy199 (07/26/20 12:33 PM)
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Fastguy199
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Re: CONTAM??? Help!! I’m an amateur! [Re: Fastguy199]
#26846057 - 07/26/20 12:38 PM (3 years, 6 months ago) |
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@goatrider I was not trying to be a douche. I in all seriousness didn’t think it would be that bad because the pictures I took were crystal clear. I’m not here to make enemies. Only friends!
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Fastguy199
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Re: CONTAM??? Help!! I’m an amateur! [Re: Goatrider]
#26846060 - 07/26/20 12:39 PM (3 years, 6 months ago) |
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I was not trying to be a douche. I am not here to make enemies.. I didn’t realize my picture was that bad! I actually thought it was a good clear picture, if it helps I will be more than happy to fix it next time! Just trying to figure out what’s going on with this dish.
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Pastywhyte
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Re: CONTAM??? Help!! I’m an amateur! [Re: Mr.Christmas]
#26846148 - 07/26/20 01:27 PM (3 years, 6 months ago) |
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Quote:
Sucio said: I, for one, love your counter. Is it fake marble? That looks like contamination, cut it out.
Cutting out the contams to save the culture on the plate is like trying to rescue a sheep that fell into a lion pen by removing the lions one at a time.
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meowjinx
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Re: CONTAM??? Help!! I’m an amateur! [Re: Fastguy199]
#26846171 - 07/26/20 01:40 PM (3 years, 6 months ago) |
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The petri dishes clearly have some contaminants. I can see what look like several little bacterial colonies
You're using a spore syringe, you're probs gonna get some contaminants on your agar. Not a big deal, that is what agar is for
You only have two choices: inoculate new plates again w/ your spore syringe and hope you get cleaner plates this time, or try and transfer what you judge to be clean mycelium from your current plates
Not sure what the brown shit is, but it's not mycelium. And not sure what you mean by "oxidation", agar shouldn't rust. It would help to know exactly what the grow media is in those dishes
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c10h12n2o
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Re: CONTAM??? Help!! I’m an amateur! [Re: meowjinx]
#26846179 - 07/26/20 01:48 PM (3 years, 6 months ago) |
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If you do a serial streak (like my sig) it will make it much easier to get a clean culture off a dirty syringe
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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Fastguy199
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Re: CONTAM??? Help!! I’m an amateur! [Re: c10h12n2o]
#26846999 - 07/26/20 11:47 PM (3 years, 6 months ago) |
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Got it, I’ve been studying it for a while now. Thanks!
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