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Shop: Unfolding Nature Unfolding Nature: Being in the Implicate Order   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies

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OfflineYeetusdeetus
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Registered: 11/23/19
Posts: 1,242
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Re: Variables that speed up lc [Re: c10h12n2o]
    #26848341 - 07/27/20 04:40 PM (3 years, 5 months ago)

Man I need to get some more media bottles so I can be picky about the lc’s I wanna use lol. Think I’m gonna go ahead and get a plate anyway since they’re not super expensive.


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Invisiblec10h12n2o
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Registered: 01/21/15
Posts: 3,200
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Re: Variables that speed up lc [Re: Yeetusdeetus]
    #26848363 - 07/27/20 05:01 PM (3 years, 5 months ago)

I just make em outta jars usually. Out of the last 50 or so ive made i cant think of but one that didnt colonize well, and im fairly sure there was a problem with the jar seal because it leaked. So no reason to test that one :smile:


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"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
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OfflineYeetusdeetus
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Registered: 11/23/19
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Re: Variables that speed up lc [Re: c10h12n2o]
    #26848425 - 07/27/20 05:29 PM (3 years, 5 months ago)

I’ve tried lc in jars but I always fumble with the metal lids for some reason. Grain jars are pretty easy but lc gets me, I know it’s the basically the same process but the lid always separates from the band when I try to pick it up. Probably just a mental thing but media bottles are always so easy for me lol.


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Invisiblec10h12n2o
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Re: Variables that speed up lc [Re: Yeetusdeetus]
    #26848455 - 07/27/20 05:48 PM (3 years, 5 months ago)

Plastic lids like blue helix uses are great. When i use metal i pry the lid off with a sterile #7 scalpel handle


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C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche


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OfflineYeetusdeetus
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Registered: 11/23/19
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Re: Variables that speed up lc [Re: c10h12n2o]
    #26848473 - 07/27/20 05:57 PM (3 years, 5 months ago)

I peel the rubber ring off so I can usually pull everything off at once if I just squeeze the band a little. I thought when I started that metal was the way to go but I’m definitely leaning towards unmodded plastic when I grab some more lol. I’d get some now but I spent like 50 bucks on these sfds and I refuse to waste them:witch:


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Offlineouuwee
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Registered: 03/22/20
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Re: Variables that speed up lc [Re: Yeetusdeetus]
    #26848489 - 07/27/20 06:03 PM (3 years, 5 months ago)

If I use pure grain water (oats) with nothing else added and no diluting, is that too much nutes? One time I tried dropping a wedge in 500 ml grain water and nothing happened. Not sure if it's the broth or maybe the wedge wasn't clean (it looked clean to me when I took the transfer)


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Invisiblec10h12n2o
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Re: Variables that speed up lc [Re: ouuwee]
    #26848502 - 07/27/20 06:12 PM (3 years, 5 months ago)

Should be way more nutrition than needed, but should still work

Id dillute it. But i usually use lme and my LCs come out crystal clear


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C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche


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InvisiblemushboyMDiscord
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Re: Variables that speed up lc [Re: c10h12n2o]
    #26848516 - 07/27/20 06:15 PM (3 years, 5 months ago)

Quote:

c10h12n2o said:
i pry the lid off with a sterile #7 scalpel handle




:brilliant:
Damn I've had issues popping jars and I know I've contaminated a few because my clumsy dirty gloved fingers struggle with the seal.


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OfflineA.k.aM
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Re: Variables that speed up lc [Re: mushboy]
    #26848585 - 07/27/20 06:52 PM (3 years, 5 months ago)

Lol I actually sterilized a bottle opener before somebody told me to pop the lid with the back of the scalpel.

I’m loving these plastic one pieces so far.


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OfflineYeetusdeetus
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Registered: 11/23/19
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Re: Variables that speed up lc [Re: A.k.a]
    #26849216 - 07/28/20 03:32 AM (3 years, 5 months ago)

What speed do you guys leave your stir plates on? I’ve heard both 60rpm and 300rpm 24/7, not sure which model I should go for either


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Invisiblesandman420
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Registered: 06/17/04
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Re: Variables that speed up lc [Re: Yeetusdeetus]
    #26849349 - 07/28/20 07:05 AM (3 years, 5 months ago)

It depends on how big your stir bar is and maybe even what specie. I tell you that I use a 2" stir bar and around 78 rpm 24/7 stir gives good results. Much slower and the mycelium builds/clogs up along the walls of the jars and stuff. 300 RPM with this stir bar makes it quite turbulent in there I dunno. I use a Chemglass Dura-mag 5 position stirrer it's pretty Cadillac. Recommend, but don't forget to order a 24v dc power adapter if you get a used one because it probably wont have it.


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