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OfflinewolfXsoul
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Registered: 06/05/20
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Is this myc or bacteria in my syringe?
    #26846526 - 07/26/20 06:04 PM (3 years, 6 months ago)

I took some old syringes, boiled water, sucked in boiling water, sat for 2 mins, expelled, repeated and then held the last in and forgot about them in my cabinet (with light) for a few days. I picked them up to move them today and noticed a few things. First, there's still black spore particle residue on the inside. Lastly, white particles floating around. I'm not sure if some rogue(very lucky) spores germinated or did somehow the boiling water end up contaminated??



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Offlineichugwindex
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Re: Is this myc or bacteria in my syringe? [Re: wolfXsoul]
    #26846643 - 07/26/20 07:21 PM (3 years, 6 months ago)

We dont usually see bacteria with the naked eye that's part of why it's such a bitch to deal with in this hobby. You can see debatable signs of it in agar tho.

Looks like mushroom mycelium to me


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Edited by ichugwindex (07/26/20 07:24 PM)


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OfflinewolfXsoul
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Re: Is this myc or bacteria in my syringe? [Re: ichugwindex]
    #26846820 - 07/26/20 08:44 PM (3 years, 6 months ago)

Interesting! I assume i can use this as a sort of LC then?


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Re: Is this myc or bacteria in my syringe? [Re: wolfXsoul]
    #26846902 - 07/26/20 09:21 PM (3 years, 6 months ago)

Personally, I pressure cook my syringes in bulk. Fill em up, wrap em in tinfoil, needle side up for 15 minutes at 20 psi in the cooker.
You should be using agar to clean things up and look for healthy growth either way.

I wouldn't try that as an LC but if you're curious try putting a drop of it in the middle of an agar dish and see what grows. If you get mycelium that means some of the spores in your syringe survived, it was an inadequate sterilization, and you had some sort of nutes in the boiling water to support growth. Maybe you made mashed potatoes and didn't wash out the pan properly aha.


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OfflinewolfXsoul
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Re: Is this myc or bacteria in my syringe? [Re: liloldme]
    #26847271 - 07/27/20 07:22 AM (3 years, 5 months ago)

Quote:

liloldme said:
Personally, I pressure cook my syringes in bulk. Fill em up, wrap em in tinfoil, needle side up for 15 minutes at 20 psi in the cooker.
You should be using agar to clean things up and look for healthy growth either way.

I wouldn't try that as an LC but if you're curious try putting a drop of it in the middle of an agar dish and see what grows. If you get mycelium that means some of the spores in your syringe survived, it was an inadequate sterilization, and you had some sort of nutes in the boiling water to support growth. Maybe you made mashed potatoes and didn't wash out the pan properly aha.




Yes! I've been working on my agar,but have been having issues with that as well. I made 25 no-pour plates and ended up with 18 of them contaminated without even inoculating. I'm not sure what I did wrong, as I pressure cooked them @12 psi for 2 hours. Plates sat for a week and came back to contam in most of em.. I used some other brand pp5 lids/containers and maybe they didn't seal correctly, I don't know..


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