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Sharp-Inflation
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Warning of working with pressure
#26845525 - 07/26/20 05:21 AM (3 years, 6 months ago) |
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Hello all,
Long story short, last night I was reheating some sterile agar that was stored inside a liquid media bottle. I began reheating the agar on a low power until it was completely liquid. Last time I did this I had some chunks in my agar that were not entirely liquefied. Therefore, I decided once the agar was liquefied that I would heat it on high for ONLY 30 seconds. This was my mistake. It caused a huge build up of pressure inside the bottle. There was no visual indication at this point, the agar was not boiling in the slightest. Yet as soon as I removed it from the microwave the cap exploded off the bottle with both going flying from my hands. The bottle bent my stovetop and caused it to stop working from the force of impact. Agar liquid covered every surface and is a huge pain in the ass to clean. Please please please learn from my mistake and be careful when working with possible pressurized containers.
I am posting this as I have learned a valuable lesson and wanted to share it as a reminder with the community. When working with sealed bottles especially liquid media bottles it is vital to ensure they do not build up too much pressure inside. Two ways of doing this are when using a PC to make sure the lid is not completely tight on the container. The second is where I made a mistake last night and it broke my stove and ended up with agar all over my kitchen and ceiling. When reheating sterile stuffs inside of a sealed container it is important to do so gradually. Set your microwave to a much lower power!! Do not use a high power under any conditions with sealed containers.
Good luck and be safe.
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Hobbyist
Citizen


Registered: 08/15/10
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I'm glad you realize you did something wrong, however I think you learned the wrong lesson. Perhaps you should try heating the agar, NOT in a sealed container. Simply heating it on lower pressure will likely achieve the same results.
I'm curious, how did a glass bottle bend your stove to the point of breaking it? I can't even imagine what part of a stove you could bend to render it non-functional.
Are you simply referencing a burner? If so, they're cheap. That was an inexpensive lesson. If somehow you did break your entire appliance(I'd like to know how), then you have my condolences for a very expensive lesson.
What guide or tek were you following that led you to microwave your media in a sealed container?
-------------------- Everything i say is completely hypothetical...
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Sharp-Inflation
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Re: Warning of working with pressure [Re: Hobbyist]
#26845600 - 07/26/20 07:04 AM (3 years, 6 months ago) |
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I had previously sterilized my agar in a media bottle with the lid loose then after it cooled I sealed the agar. I was trying to reheat it. I thought opening the lid while reheating it may allow for contaminants to enter the bottle?
Well it bent the prongs of the stovetop. Then when I look inside the gas stove the little flame inside is gone and the clicking sounds no longer happens when turning the burner on (the burner does not actually turn on, just turning the handle for it). If it is broke I live in an apartment so my realty company will take care of it and in the meantime I can just cook at my girls place.
I was following Bods comprehensive agar tek. https://www.shroomery.org/forums/showflat.php/Number/21922023
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Hobbyist
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I see that he doesn't specifically say to loosen the lid before reheating it. I can see why you would thing that.
Something to think about - People use unmodified plastic lids for grain jars, they just loosen them a little so that they have gas exchange between the threads.
-------------------- Everything i say is completely hypothetical...
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Sharp-Inflation
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Re: Warning of working with pressure [Re: Hobbyist]
#26845626 - 07/26/20 07:31 AM (3 years, 6 months ago) |
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So just to double check you are saying that I should not have to worry about contams if I just crack the lid a little on my jars when I nuke them in the microwave?
Thank you for all your assistance!
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Hobbyist
Citizen


Registered: 08/15/10
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Hmm, What am I saying? I suppose it's only fair to be clear.
It's my opinion that saving sterilized agar to pour later is not best practice.
If you do intend to save sterilized agar to pour later, you of course can, and many do with good results.
If you're using a media bottle or another type of bottle where the lid fits over the top of the bottle opening and has threads down the sides and the lid is of one piece, then I wouldn't worry about contamination if you loosen the lid enough to allow airflow while microwaving.
To be clear, I wouldn't move the bottle around over much after loosening the lid as drafts or airflow against it is going to lead to higher likelyhood of contam-laden air making it's way up through those threads(ie:don't loosen the lid and go running through a field with it or wave it violently around).
I wouldn't use a mason jar and set the two piece lid off center hoping to keep it sterile and allow airflow.
Many people use an old liquor bottle with a piece of foil over the top. This wouldn't make a seal and as such you can microwave it as is.
-------------------- Everything i say is completely hypothetical...
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ItsaMeShroomio
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Re: Warning of working with pressure [Re: Hobbyist]
#26845685 - 07/26/20 08:23 AM (3 years, 6 months ago) |
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Start microwaving with the lid sealed, once it's at positive pressure, loosen the cap and continue.
If you waited for the agar to cool to room temp before sealing the lid, just nuke it for 30 seconds then loosen it. Otherwise, make sure its as hot as it was when you originally sealed the lid and there will be positive pressure and no contams will suck in when you loosen the cap. Just make sure to reseal it while you're waiting for it to cool down to pour.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
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I think even kids know. Everything in the microwave needs a loose lid.
Microwave makes things hot. Sealed containers bad.
Media bottle with lid tightened down in a microwave sounds like potential bomb
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bodhisatta 
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Re: Warning of working with pressure [Re: bodhisatta]
#26845719 - 07/26/20 08:50 AM (3 years, 6 months ago) |
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No contamination sucks into a boiling hot jar people.
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Re: Warning of working with pressure [Re: bodhisatta]
#26845727 - 07/26/20 08:55 AM (3 years, 6 months ago) |
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Also With Pyrex you have to be careful of superheating liquids which sounds like is what happened. It’s so smooth it can get above boiling without bubbling and basically becomes a bomb the second you disturb it.
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LAGM2020     
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Sharp-Inflation
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Re: Warning of working with pressure [Re: A.k.a]
#26845731 - 07/26/20 09:00 AM (3 years, 6 months ago) |
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Thanks for the replies, I just finished getting everything all cleaned up in time for my gf to return today! This was a valuable lesson learned, also maybe don't do your agar work baked or you make dumbass choices without thinking through the consequences. I will be doing better in the future and thanks again to everyone who chimed in.
I forgot to mention the part about how I jumped backwards, tripped on the vacuum, and went ass over teakettle.
Edited by Sharp-Inflation (07/26/20 09:05 AM)
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CrashTest
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I’m still not understanding the part about how a glass media bottle bent the prongs on your stove?
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this account is automated, any posts related to activities or advice thereof are strictly opinions from numerous online sites and are for informational purposes only - CrashTest2020
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bodhisatta 
Smurf real estate agent


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Re: Warning of working with pressure [Re: CrashTest]
#26845778 - 07/26/20 09:36 AM (3 years, 6 months ago) |
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Bent the stovetop itself is what he said. The bottle exploded downwards and bent the sheet metal or a burner
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Sharp-Inflation
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Re: Warning of working with pressure [Re: bodhisatta]
#26845793 - 07/26/20 09:44 AM (3 years, 6 months ago) |
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Lmao, yeah so I took it out of the microwave and the cap and bottle exploded out of my hand. I think the bottle smashed the burner and I tried to bend it last night but was so pissed off I said screw it. Yes I know the stove is dirty but here is a pic. I am really shocked nobody else is finding this shit as funny as I do...

Also while you are here any recs on what to do about these very dark caps? Are these aborts you think? I am guessing low fae caused these bad boys to "suffocate"..

 Thanks
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bodhisatta 
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Probably too wet
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Sharp-Inflation
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Re: Warning of working with pressure [Re: bodhisatta]
#26845817 - 07/26/20 09:53 AM (3 years, 6 months ago) |
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You think the substrate was too wet? I would be a little surprised to hear that but alright thanks I will give it some more fae and not mist.
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CrashTest
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Re: Warning of working with pressure [Re: bodhisatta]
#26845819 - 07/26/20 09:54 AM (3 years, 6 months ago) |
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HAHAHA sorry I just laughed out loud that shit is hilarious. What I am imagining is an absolute hilarious scenario in the kitchen. I’d be more mad that I broke my media bottle
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this account is automated, any posts related to activities or advice thereof are strictly opinions from numerous online sites and are for informational purposes only - CrashTest2020
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Sharp-Inflation
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Re: Warning of working with pressure [Re: CrashTest]
#26845834 - 07/26/20 09:59 AM (3 years, 6 months ago) |
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Thank you finally someone gets it! haha the best part is that the media bottle is completely fine and not even cracked...
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CrashTest
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Quote:
Sharp-Inflation said: Thank you finally someone gets it! haha the best part is that the media bottle is completely fine and not even cracked...
Wow hahah that’s insane. I’m waiting for my PC to sterilize my agar in my media bottles but I’m gonna loosen the caps lol
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this account is automated, any posts related to activities or advice thereof are strictly opinions from numerous online sites and are for informational purposes only - CrashTest2020
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Re: Warning of working with pressure [Re: CrashTest]
#26845847 - 07/26/20 10:05 AM (3 years, 6 months ago) |
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I had something similar happen only the bottom of the media bottle blew out in a perfect circle instead of the cap shooting off.
Good old cheap bottles.
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LAGM2020     
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Sharp-Inflation
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Re: Warning of working with pressure [Re: CrashTest]
#26845848 - 07/26/20 10:05 AM (3 years, 6 months ago) |
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Just about to do the same myself! Got to make up some fresh agar after last nights incident. I really shouldn't delay my transfers any longer, but scrolling shroomery after a smoke is such a peaceful way to relax.
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Sharp-Inflation
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Re: Warning of working with pressure [Re: bodhisatta]
#26845862 - 07/26/20 10:20 AM (3 years, 6 months ago) |
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Holy shit, you're Bod... wow thank you for your time I am sorry I doubted you about the water content. Thank you and I am sorry again.
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bodhisatta 
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Lol, no problem man. I'm just a guy
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Sharp-Inflation
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Re: Warning of working with pressure [Re: bodhisatta]
#26845870 - 07/26/20 10:24 AM (3 years, 6 months ago) |
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A guy who puts the time in to write brilliant write ups (minus forgetting to say to loosen the lid on the media bottle lmao) and puts a lot of time into this community, its impressive so thank you. I am going to go reread your comprehensive agar tek and get to work.
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