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OfflineSharp-Inflation
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Second Agar Transfer, where to transfer
    #26844191 - 07/25/20 08:34 AM (3 years, 6 months ago)

Good morning,

I am preparing to take the second transfer. I took two transfers from the original clones which are labeled A1 and A2 and B1 and B2. I am curious which plate and where I should take transfers. I am hoping to take only one or two of these first transfers and take transfers due to a limited number of petri dishes.



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InvisiblebodhisattaMDiscordReddit
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Re: Second Agar Transfer, where to transfer [Re: Sharp-Inflation]
    #26844203 - 07/25/20 08:41 AM (3 years, 6 months ago)

Why are your dishes all covered in plastic?

Take transfers from whatever you think looks best


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OfflineGan
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Re: Second Agar Transfer, where to transfer [Re: bodhisatta]
    #26844223 - 07/25/20 08:52 AM (3 years, 6 months ago)

Plate A: regions of strong growth
Plate A2: 7-9 and 10-12
Plate B: same as plate A. Any of those regions with good looking growth
Plate B1: 6-8 and 12-2
Plate B2: 7-11:30


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OfflineSharp-Inflation
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Re: Second Agar Transfer, where to transfer [Re: Gan]
    #26844696 - 07/25/20 02:00 PM (3 years, 6 months ago)

Thanks for the advice Gan. I will be taking transfers from A2 and B2 later today. Is there any way to guess how many more transfers I should take until I drop some agar onto grains? (specifically rye berries! trying them for the first time after the agar is ready!)


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Offlinew00tmycelium
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Re: Second Agar Transfer, where to transfer [Re: bodhisatta]
    #26844735 - 07/25/20 02:20 PM (3 years, 6 months ago)

Quote:

bodhisatta said:
Why are your dishes all covered in plastic?

Take transfers from whatever you think looks best




I'm a big fan of throwing my plates in ziplocs for humidity retention and seems to keep them isolated from bacterial contams on the sides.  Parafilm would probably be way better but I figure why not wrap in plastic if you've got it around.


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Offlinew00tmycelium
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Re: Second Agar Transfer, where to transfer [Re: Sharp-Inflation]
    #26844744 - 07/25/20 02:24 PM (3 years, 6 months ago)

Quote:

Sharp-Inflation said:
Thanks for the advice Gan. I will be taking transfers from A2 and B2 later today. Is there any way to guess how many more transfers I should take until I drop some agar onto grains? (specifically rye berries! trying them for the first time after the agar is ready!)




You'd be fine anywhere from T1 through T4 as far as I can tell to dump onto grains.


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OfflineGan
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Re: Second Agar Transfer, where to transfer [Re: Sharp-Inflation]
    #26844798 - 07/25/20 03:13 PM (3 years, 6 months ago)

Quote:

Sharp-Inflation said: Is there any way to guess how many more transfers I should take until I drop some agar onto grains? (specifically rye berries! trying them for the first time after the agar is ready!)




Not really a way to guess. They're just ready to go to grain when they're clean. Could be the first transfer or it could be many down the road.


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