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Ballzagna
Carb Daddy



Registered: 10/09/19
Posts: 619
Last seen: 13 days, 13 hours
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Where to get good slant tubes?
#26843707 - 07/24/20 10:19 PM (3 years, 6 months ago) |
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TL;DR - bad luck with culture tubes having shitty lids that melt in the PC, where can I get good culture tubes with PP lids?
I've tried my hand at frank's slant tek a few times but every time the lids melt in the PC leaving the tubes unscathed. I ordered a small bag of tubes on amazon to give it a whirl and the first batch I thought I put the lids too tight/PC'd too hot or too long. The second batch I corrected those variables and still got the same issue, contacted the seller and found out the lids weren't PP just the tubes and that info was hidden in the description, disingenuous but fair enough.
So I bit the bullet and bought a 500ct from a science supply website, scouring the listing page to see if they specify lid material (they didn't) but gives the option when you purchase the tubes to get either polystyrene or PP, I chose PP with the expectation the lids would match, but now after doing them up tonight these fucking lids melted too and I am displeased.
Does anyone know any good places to get culture tubes that come with lids that aren't going to melt?
-------------------- No one likes a naysayer It's probably fine. Even if it's not fine, it'll eventually be fine. So it's fine. PE Adventure
Photosynthesis: A Morning Ritual Ballzagna's Tek Compendium and Notes for Noobs 
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Bsdgaou

Registered: 02/20/19
Posts: 340
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Re: Where to get good slant tubes? [Re: Ballzagna]
#26843789 - 07/24/20 11:25 PM (3 years, 6 months ago) |
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There are some glass tubes, but ... I would like them in 20-30mm size. Most of them are 10mm in diameter.
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Forrester
aspiring sociopath


Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA
Last seen: 24 days, 18 hours
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Re: Where to get good slant tubes? [Re: Bsdgaou]
#26844014 - 07/25/20 05:22 AM (3 years, 6 months ago) |
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I think it was something like these I used to use... never had a problem with the lids.
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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jcm4620
Stranger


Registered: 05/26/19
Posts: 6,700
Last seen: 1 year, 11 months
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Re: Where to get good slant tubes? [Re: Forrester]
#26844069 - 07/25/20 06:27 AM (3 years, 6 months ago) |
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i use the 50 ml centrifuge tubes like these
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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,384
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Re: Where to get good slant tubes? [Re: jcm4620]
#26844073 - 07/25/20 06:30 AM (3 years, 6 months ago) |
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You can get 50 cc centrifuge tubes that also have a flat bottom so they stand up.
Now don't forget to add a few wood sticks in there like toothpicks or "hobby sticks"
Forget about the growth on the agar surface, you only want to pluck out the sticks.
They last, the agar growth can die in bad storage or over time I have found.
The wood stick though, just plop it on agar it will grow...
I like to add 3 or 4 small wood sticks in the slant at least and place the OG agar transfer right where the wood and agar meet to start growing and colonize the wood especially.

but I like these smaller wood sticks that I can just pluck out and play on a plate.
Edited by sandman420 (07/25/20 06:45 AM)
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Doyledozo
Humble student



Registered: 10/22/19
Posts: 156
Last seen: 7 months, 18 days
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Re: Where to get good slant tubes? [Re: sandman420]
#26844110 - 07/25/20 07:21 AM (3 years, 6 months ago) |
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I use the same ones JCM has except mine are 15ml I think. They even came with free pipettes. Almost tossed those until I started doing extractions, then they came in super handy.
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Buddaking
Genetics



Registered: 12/18/09
Posts: 1,775
Loc: No Mans Land
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Re: Where to get good slant tubes? [Re: Doyledozo]
#26844127 - 07/25/20 07:34 AM (3 years, 6 months ago) |
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Got thease a few monthsago on Amazon for about 25 usd delivered.. They have held up fine in my PC... Did not shop around so don't know if it was a good deal or not.
--------------------
 AMU Q & A
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Doyledozo
Humble student



Registered: 10/22/19
Posts: 156
Last seen: 7 months, 18 days
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Re: Where to get good slant tubes? [Re: Buddaking]
#26844188 - 07/25/20 08:31 AM (3 years, 6 months ago) |
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CrashTest
Stay Learning


Registered: 06/21/20
Posts: 623
Last seen: 6 months, 29 days
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Re: Where to get good slant tubes? [Re: Doyledozo]
#26844300 - 07/25/20 09:40 AM (3 years, 6 months ago) |
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Subbed to this thread for later, thanks for the tips guys
--------------------
this account is automated, any posts related to activities or advice thereof are strictly opinions from numerous online sites and are for informational purposes only - CrashTest2020
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