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Offlinejlr90
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First Grow - Unbodified Mono Tub [Update]
    #26843332 - 07/24/20 06:53 PM (3 years, 6 months ago)

Thank you to the contributors of this wicked website! I'm having alot of fun with this hobby thus far and this place is a weapon of a resource.

I decided to go with bods unmodified mono for my first crack at it.

Everything has gone smoothly so far. 6 days ago I transferred 6qt of fully spawned oats into a 54qt sterilite. My 800g of coir was leaking a few drops of water with a really hard squeeze after Bods coir tek. Mixed the Spawn into the substrate and packed it in nice and level, alittle extra on the sides and corners. I am running a HEPA air purifier in the room. The tub sits in a room in natural indirect sunlight at around 75f-85f and 50%-60% conditions inside the tub with the lid upside down.

From day 2 to day 3 the surface got noticeably dryer than when i first started and up to today the substrate has shrunk a little bit. There is condensation where the substrate meets the tub, nothing along the wall of the tub above the substrate.

My main focus is on surface conditions at this point, but i dont have the "green thumb" and given this is my first crack i have no idea what to try to maintain the surface at.

I bought distilled water and a spray bottle day 5 and day 6 began spraying the sides of tub as well as a few light sprays directly on the surface.

What do you think/any critique on my setup? At this stage what is optimal surface conditions look like? What should I be aware of at this stage/any tips? Is my RH inside the tub sufficient?

Here's a photo of my surface .



Edited by jlr90 (08/02/20 12:36 PM)


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Offlinejcm4620
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Re: First Grow - Surface Conditions - Unbodified Mono Tub [Re: jlr90]
    #26843556 - 07/24/20 08:44 PM (3 years, 6 months ago)

well only thing i can suggest at this point would be next time try to cover your exposed grains with a top layer of only sub. surface conditions is gnna come more into play once you have a colonized sub


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Offlinejlr90
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Re: First Grow - Surface Conditions - Unbodified Mono Tub [Re: jcm4620]
    #26843608 - 07/24/20 09:12 PM (3 years, 6 months ago)

Okay I'll try that for the next go. In Bods Tek he says he doesn't mind having exposed oats on the surface. Do you think I should continue misting 1 to 2 times a day?


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InvisibleHobbit GDF
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Re: First Grow - Surface Conditions - Unbodified Mono Tub [Re: jlr90]
    #26843813 - 07/24/20 11:39 PM (3 years, 6 months ago)

I wouldn't mist unless the coir changes color like its dry. Besides that let it colonize all the way and then the moisture will appear on the surface.  Really it's just wait game until then. Normally dont need to mist before first flush if you put some leftover substrate on top.


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InvisibleHobbit GDF
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Re: First Grow - Surface Conditions - Unbodified Mono Tub [Re: Hobbit GDF]
    #26843815 - 07/24/20 11:40 PM (3 years, 6 months ago)

It's been 6 days and that's how it looks? That's not good. That pic looks like day 1.


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InvisiblePastywhyteMDiscord
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Re: First Grow - Surface Conditions - Unbodified Mono Tub [Re: Hobbit GDF]
    #26843847 - 07/25/20 12:17 AM (3 years, 6 months ago)

If that’s been six days since spawning it’s nothing more than trich food at this point.


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Invisiblec10h12n2o
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Re: First Grow - Surface Conditions - Unbodified Mono Tub [Re: Pastywhyte]
    #26843927 - 07/25/20 02:27 AM (3 years, 6 months ago)

:whathesaid:


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Offlinejlr90
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Re: First Grow - Surface Conditions - Unbodified Mono Tub [Re: Pastywhyte]
    #26846092 - 07/26/20 12:59 PM (3 years, 6 months ago)

Quote:

Hobbit GDF said:
I wouldn't mist unless the coir changes color like its dry. Besides that let it colonize all the way and then the moisture will appear on the surface.  Really it's just wait game until then. Normally dont need to mist before first flush if you put some leftover substrate on top.




Quote:

Pastywhyte said:
If that’s been six days since spawning it’s nothing more than trich food at this point.




Nooooo! shit, alright where do you guys think I went wrong on? What could i do to improve? Or what information would you need to diagnose my errors?


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InvisiblePastywhyteMDiscord
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Re: First Grow - Surface Conditions - Unbodified Mono Tub [Re: jlr90]
    #26846123 - 07/26/20 01:16 PM (3 years, 6 months ago)

It’s likely contaminated spawn. A detailed run down of what you did to make it along with pics of it would help. Most new people can’t tell if their spawn is bacterial unless it stinks to hell. Often it’s far more sneaky than that.


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OfflineA.k.aM
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Re: First Grow - Surface Conditions - Unbodified Mono Tub [Re: Pastywhyte]
    #26846134 - 07/26/20 01:20 PM (3 years, 6 months ago)

It’s gotta be some gnarly bacteria to not colonize at all too.

That sucks man I know the feeling. It took me a while to realize clean spawn is 90% of cult, sterile technique is by far the most important aspect of this stuff and I still go back and read/watch the videos on basics.


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InvisiblePastywhyteMDiscord
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Re: First Grow - Surface Conditions - Unbodified Mono Tub [Re: A.k.a]
    #26846137 - 07/26/20 01:22 PM (3 years, 6 months ago)

He doesn’t mention temps but if it got up into the 100’s that could have killed the colony. Otherwise it’s likely nasty spawn from the get go. I personally dislike oats as well.


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OfflineDTH
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Re: First Grow - Surface Conditions - Unbodified Mono Tub [Re: Pastywhyte]
    #26846348 - 07/26/20 03:36 PM (3 years, 6 months ago)

For context, Here is (what you can see) my first tub after 24 hours of colonization.

I have it covered near my apartment window for more heat. I'm scared to open the lid so I just took this photo.


Edited by DTH (07/26/20 03:37 PM)


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InvisiblePastywhyteMDiscord
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Re: First Grow - Surface Conditions - Unbodified Mono Tub [Re: DTH]
    #26846362 - 07/26/20 03:44 PM (3 years, 6 months ago)

Opening the lid won’t hurt a thing. A burst of fresh air is good for mushrooms and bad for many contams.


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OfflineDTH
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Re: First Grow - Surface Conditions - Unbodified Mono Tub [Re: Pastywhyte]
    #26846367 - 07/26/20 03:47 PM (3 years, 6 months ago)

I think I've read that before, as well that spawning doesnt have stringent sterility requirements, but I'm trying to be extremely careful!


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Offlinejcm4620
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Re: First Grow - Surface Conditions - Unbodified Mono Tub [Re: DTH]
    #26846420 - 07/26/20 04:33 PM (3 years, 6 months ago)

well in being careful u can be killing it so 🤷🏼🤷🏼


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InvisibleHobbit GDF
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Re: First Grow - Surface Conditions - Unbodified Mono Tub [Re: jcm4620]
    #26846959 - 07/26/20 10:39 PM (3 years, 6 months ago)

Clean spawn and you can be dirty after that. It's realy cool


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Offlinejlr90
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Re: First Grow - Surface Conditions - Unbodified Mono Tub [Re: Pastywhyte]
    #26850346 - 07/28/20 05:29 PM (3 years, 6 months ago)

Quote:

Pastywhyte said:
It’s likely contaminated spawn. A detailed run down of what you did to make it along with pics of it would help. Most new people can’t tell if their spawn is bacterial unless it stinks to hell. Often it’s far more sneaky than that.




Thanks Pasty

After following bods oat tek, Ill detail the process starting from removing from the pressure cooker. Unfortunately i didn't take photos of the spawn. There were no colors, just pastywhite. I inoculated the jars by injecting the them with the spore solution, which i now know can increase likelihood of contam. I didnt think to smell the jars because all the work i did with the jars was inside the SAB until i mixed it with the substrate. I did try another inoculation method, which was fun, in the process i did G2G to increase the amount of jars i needed. The temp never exceeded 87F. RH in the room was 40%

  • Clean and disinfect room, SAB, and Gloves (Iso the gloves multiple times during this process)
  • Remove jars from pressure cooker, into SAB close lid
  • Assemble jars inside SAB
  • Wipe injection ports with alcohol
  • Assemble and flame sterilize syringe outside of SAB
  • Inject 3 jars with 3cc/each
  • I left the jars undisturbed inside the SAB, in a closet for a 10 days then
  • I decided to up the size of my tub from 32qt to 54qt, i realized i needed more grain spawn. I wanted to see how much faster a jar would colonize if i used a whole syringe into an extra jar i made.
  • I made 3 more spawn jars repeating the same procedure.
  • When the spawn jar that i inoculated with a full syringe was nearly fully colonized, i did a 1/3 G2G transfer to the 3 new spawn jars i made.
  • The original jars and the g2g jars werent on the same pace at this point. The g2g jars were a little bit behind, so the original jars sat fully colonized for an additional 10 days until the g2g jars were fully colonized.
  • Turned the HEPA air filter on in the room I was going to be mixing in
  • I pasteurize my substrate using bods tek again. I let 800g of coir sit in the cooler for 10 hours before using it. The coir let a a few drops out with a hard prolonged squeeze
  • I mixed the substrate with the grain spawn in a clean disinfected room, made the surface level and slightly packed more around the edges than the middle.
  • Put the lid on upside down and set in a spot with indirect natural light close to the HEPA filter.


Since my first post ive got side pins going on as well.


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Offlinejlr90
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Re: First Grow - Surface Conditions - Unbodified Mono Tub [Re: A.k.a]
    #26850354 - 07/28/20 05:32 PM (3 years, 6 months ago)

Quote:

A.k.a said:
It’s gotta be some gnarly bacteria to not colonize at all too.

That sucks man I know the feeling. It took me a while to realize clean spawn is 90% of cult, sterile technique is by far the most important aspect of this stuff and I still go back and read/watch the videos on basics.




I'll go back and review as well thanks dude.


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Offlinejlr90
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Re: First Grow - Surface Conditions - Unbodified Mono Tub [Re: Hobbit GDF]
    #26850363 - 07/28/20 05:35 PM (3 years, 6 months ago)

Quote:

Hobbit GDF said:
I wouldn't mist unless the coir changes color like its dry. Besides that let it colonize all the way and then the moisture will appear on the surface.  Really it's just wait game until then. Normally dont need to mist before first flush if you put some leftover substrate on top.




So i did notice the surface starting to dry out at about day 2. Do you think maybe I didnt have the substrate at field capacity? the room was at around 85f.

And When you say let it colonize, does that mean having the lid closed? Just trying to understand if you mean let it colonize with the lid upside down or not.


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Offlinejlr90
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Re: First Grow - Surface Conditions - Unbodified Mono Tub [Re: jlr90]
    #26858795 - 08/02/20 12:35 PM (3 years, 6 months ago)

Hey guys,

As i mentioned i was getting some side pins on the sub, I decided to try to grow them because i figured my tub was a lost cause anyway. Mis well get some experience out of it.

I took a clean sharp knife, wiped it down with some iso and cut the sides off the block of sub and put them into fruiting condition.

They grew!

But i noticed they had some white specs on the cap as shown in the photos. Is this normal?





Would you say its time to harvest these? Some of the veils have dropped already. What do you suggest??

Went a head and started making my first prints! Do you think 4 is enough or should i make more?




Since I thought the tub was a lost cause because it wasn't colonizing, misted the tub and sealed the lid to see what would happen. Here is where its at:



I dont see any green on it and it seems to smell okay.

What do you think, can i fruit this?:thumbup:


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