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starbones
I'm an alien, I eat uranium.



Registered: 03/04/20
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Are 0.5 micron unicorn bags absolutely useless if trich is around?
#26842999 - 07/24/20 04:07 PM (3 years, 6 months ago) |
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Lost 4 out of 9 bags with 4qts each per bag
All bags inoculated through the bag with a fresh 18g needle for each one. LC was clean on agar. inoculation was done in front of my flow hood.
I notice it is extremely easy to pull air into these bags through the filter. I preseal and PC them for e.5 hours.
They all had the same LC from the same 50ml syringe and other bags are fine.
Trich is smaller than 0.5 so I am wondering if its best to take a loss on the stack of 0.5 bags and switch to 0.2 IF the filter is the problem
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sandman420
Saint PP



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Re: Are 0.5 micron unicorn bags absolutely useless if trich is around? [Re: starbones]
#26843070 - 07/24/20 04:59 PM (3 years, 6 months ago) |
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No I use them no problem.
If it is "extremely easy" to pull air in them, you air not getting a good seal or have a hole.
I bet you are not getting a good seal where the gussets have that extra layer on the inside.
Try to peel the seal flaps at various gussets in between the 2 extra thick layers at the ends... and see if any are loose I bet you find a problem...
bags are hard. more to go wrong. I use 0.5 with very little contams in a dirty environ.
Edited by sandman420 (07/24/20 05:17 PM)
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sandman420
Saint PP



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Re: Are 0.5 micron unicorn bags absolutely useless if trich is around? [Re: sandman420]
#26843108 - 07/24/20 05:20 PM (3 years, 6 months ago) |
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The pro way to seal bags is actually the gussets folded all the way out so the seal is 1 single layer
Need a 16" sealer for the large unicorn bags though.
This makes the air space in the bag way way bigger too for a better shake.
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starbones
I'm an alien, I eat uranium.



Registered: 03/04/20
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Last seen: 3 months, 4 days
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Re: Are 0.5 micron unicorn bags absolutely useless if trich is around? [Re: sandman420]
#26843291 - 07/24/20 06:38 PM (3 years, 6 months ago) |
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Ok, I will try to achieve a better seal. Could I not just seal, seal again on the same spot?
Or seal and then just use packing tape on the top?
-------------------- Listen, I'm steel fisted with the iron lung Heavy metal ballads out the guitar where lions run.
 
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starbones
I'm an alien, I eat uranium.



Registered: 03/04/20
Posts: 1,131
Last seen: 3 months, 4 days
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Re: Are 0.5 micron unicorn bags absolutely useless if trich is around? [Re: sandman420]
#26843319 - 07/24/20 06:47 PM (3 years, 6 months ago) |
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Like such? Ignore the other seal I misaligned it.
-------------------- Listen, I'm steel fisted with the iron lung Heavy metal ballads out the guitar where lions run.
 
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sandman420
Saint PP



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Re: Are 0.5 micron unicorn bags absolutely useless if trich is around? [Re: starbones]
#26843989 - 07/25/20 04:48 AM (3 years, 6 months ago) |
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Did you try to test the seals on your bad bags and see if any are loose like I said?
If you seal and seal again in the same spot it is possible to burn through.
That could also be happening, you could have the heat too high and be burning a small hole int he seal in some spot.
One thing is sure, if you can easily inflate the bags they have a hole or a bad seal.
They should take hours to inflate by pulling the top up if uncompromised.
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Forrester
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Re: Are 0.5 micron unicorn bags absolutely useless if trich is around? [Re: sandman420]
#26844011 - 07/25/20 05:19 AM (3 years, 6 months ago) |
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I agree the filter is probably not the problem. Although, I have seen before when growing shiitake in the .5 bags, if green did form during the long incubation, it would form right under the filter patch.
So there's some conflicting information.
If you can't nail it down to a sealing issue and wanna just try a new bag, don't toss the .5 bags just use 'em for substrate.
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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sandman420
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Re: Are 0.5 micron unicorn bags absolutely useless if trich is around? [Re: Forrester]
#26844017 - 07/25/20 05:29 AM (3 years, 6 months ago) |
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Not 100% successful you are right. Something like ~5% contam is common for me on top or around the patch area. So "no problem" wasn't the right words to use, instead "They Work 90+% of the Time, Every time"
But I find a lot of the contam bags are because of the seal actually.
The gusset part folded in adds an extra 2 layers of plastic that the impulse sealer must burn through, while not being too hot to burn through the part of the seal that is only 2 layers thick.
The outer parts of the seal with 4 layers of plastic can sometimes not actually get a seal on on the inner part.
So you sometimes get a situation where there is a little flap of plastic that looks sealed but is actually letting some air in.
Signs of this are tops of the bags not colonizing and top contams.
You can peel between the plastic layers of the seal with some force to test it out.
Edited by sandman420 (07/25/20 06:27 AM)
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sandman420
Saint PP



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Re: Are 0.5 micron unicorn bags absolutely useless if trich is around? [Re: sandman420]
#26844097 - 07/25/20 07:01 AM (3 years, 6 months ago) |
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Also I meant like this
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Buddaking
Genetics



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Re: Are 0.5 micron unicorn bags absolutely useless if trich is around? [Re: sandman420]
#26844137 - 07/25/20 07:43 AM (3 years, 6 months ago) |
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I only fruit in .5 micron A type filter bag. I expierence a 1% to 2% rate of green I attribute to my technique. I have recently had a horrible green one I missed stuffed right up aginist a good one in the back & expierenced no cross contamination. I run spawn in .2 T type filter bags. I never disturb my fruit bags to eliminate bag separation from substrate as that encourages side pins in the micro climate created.
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