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OfflineDonkDonk
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Registered: 04/12/20
Posts: 59
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PF Jars...no action??
    #26842678 - 07/24/20 01:14 PM (3 years, 6 months ago)

Hey,

I inoculated 6 Pf Tek jars with PF Classic 11 days ago. There is no signs of growth. I have never had this happen before. Should spores always be visible in the syringe? I don't see any but I do in the other 5 syringes I bought. Very reputable seller.

I also knocked up grain jars with B+ and they are growing strong.

I used 1cc for each PF jar so I have half a syringe left. What do you think about using the remainder on the jars at this stage? If I do this should I sterilize the jars again or start from scratch. Or should I just leave them another 10 days?


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OfflineCamera93
We got dicks like Jesus
I'm a teapot


Registered: 08/15/18
Posts: 3,220
Last seen: 10 days, 12 hours
Trusted Cultivator
Re: PF Jars...no action?? [Re: DonkDonk]
    #26842684 - 07/24/20 01:19 PM (3 years, 6 months ago)

It looks like it can take up to 14 days

EvilMushroom666's Take on BRF Cakes


you're waiting for the spores to germinate, even on agar some people report upwards of 2 weeks for some older prints

Quote:

EvilMushroom666 said:
Part 4 - Colonization

After inoculation you will want to place your jars on a shelf and leave them alone. Most people new to the hobby want to move and check their babies every few hours. This will be detrimental for your cakes. Your dry vermiculite barrier acts as a filter, and the more you move and disturb your cakes, the bigger chance you will shift your vermiculite barrier and let contaminates into your cake.

Room temperature is fine for colonization (70-75F, If you are comfortable, your cakes will be too), you DO NOT need any fancy incubators or the like. Cakes will colonize fine at temperatures even lower then room temp (60-70F) but will progress a little slower. If you are worried about keeping your temperature around 70-75F a small space heater can be used to do the job. Old outdated information has circulated the internet about the ideal temperature for cube growth being 86F, this is flat out untrue and outdated. Temperatures above 80F can promote bacterial and mold growth and germinate nasty contaminates that may not have germinated had the temperatures been kept a bit lower.Also you do not need to keep your jars in complete darkness, place your jars on a shelf and leave them be, ambient light is fine.

Spore germination should take place in 7-14 days, time varies. Patience is key at this point, and you are better off to forget about your jars for at least 10 days before checking them all individually.






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