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OfflineM.Dana
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Registered: 07/24/20
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Last seen: 3 years, 5 months
Is this monoculture?
    #26841977 - 07/24/20 04:38 AM (3 years, 6 months ago)

even after seeing a lot of monoculture pics I still can't tell, they all seem unique :confused: for example is there sectoring in 2o'clock?


Edited by M.Dana (07/24/20 04:41 AM)


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Offlinescarabaeus
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Registered: 06/13/18
Posts: 1,101
Last seen: 2 years, 1 month
Re: Is this monoculture? [Re: M.Dana]
    #26841988 - 07/24/20 05:04 AM (3 years, 6 months ago)

You have an awesome rhizomorphic plate. This is a transfer from a multispore plate I'm guessing? Yes, at 2 O'clock there is a sector.  But it doesn't matter. No matter where you take wedges from to inoculate grains, that wedge will contain lots of strains. Even a tiny piece of your plate from anywhere on it will contain several if not many strains. Mycelium tends to sort itself out -compatible strains get together and stay together -they all behave similarly (as far as a grow is concerned). You don't want a single strain unless you are doing some serious (pro) lab work. Single strains are hard to maintain - they easily lose their vigor and die out.
A wedge that contains multiple strains that are compatible (like everything you have on your plate) means that genetic material is being exchanged between the various strains as they rub shoulders. This process is what keeps the culture vigorous and healthy. The job of mycelium is to eat, grow and mutate in an effort to stay alive. It needs compatible friends (strains) to do this.  An actual single strain is hung out to dry because it has no buns to bump with -a lonley place :begger:

Hope I'm not rambling. Hope I gave you sumpin' to think about :headbang:


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OfflineAtmozFear
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Registered: 01/25/19
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Re: Is this monoculture? [Re: scarabaeus]
    #26841998 - 07/24/20 05:23 AM (3 years, 6 months ago)

great info :takingnotes:


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Offlinescarabaeus
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Re: Is this monoculture? [Re: AtmozFear]
    #26842001 - 07/24/20 05:30 AM (3 years, 6 months ago)

Thanks -nice grow in your pic ^ .


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Offlineredhandmat
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Registered: 05/09/19
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Re: Is this monoculture? [Re: AtmozFear]
    #26842003 - 07/24/20 05:31 AM (3 years, 6 months ago)

Yeah what he said. Also, you can just grow your mushrooms and clone those fruits that look nice to you. And frankly, if you are not willing to separate each strain from a MS and test if for potency, speed, agility etc then there is no point in even having a monoculture. You can literally end up having the worst strain of the bunch you have right now. That would suck :frown: So just keep working with what you have now, and select the fruits that you like to get more and more of those.


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OfflineAtmozFear
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Re: Is this monoculture? [Re: redhandmat]
    #26842043 - 07/24/20 06:11 AM (3 years, 6 months ago)

Quote:

scarabaeus said:
Thanks -nice grow in your pic ^ .



thanks, first comment on it I ever got... there would have been a canopy but I am home 24-7 (disability) and I am able to pick each and every one as soon as the veils break.  Am also able to use unmodded monotubs because I am always home, so I can move/remove the tops whenever I need to.

Quote:

redhandmat said:
Yeah what he said. Also, you can just grow your mushrooms and clone those fruits that look nice to you. And frankly, if you are not willing to separate each strain from a MS and test if for potency, speed, agility etc then there is no point in even having a monoculture. You can literally end up having the worst strain of the bunch you have right now. That would suck :frown: So just keep working with what you have now, and select the fruits that you like to get more and more of those.



thanks guys, great tips this morning :goodmorning:


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Offlinemushpunx
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Registered: 04/20/14
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Re: Is this monoculture? [Re: redhandmat]
    #26842045 - 07/24/20 06:11 AM (3 years, 6 months ago)

I see sectoring yeah. Try turning it around and looking at it thru the back , it's easier to spot sectors



--------------------

Amateur Mycologists United
AMU Q&A


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Offlinescarabaeus
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Registered: 06/13/18
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Re: Is this monoculture? [Re: AtmozFear]
    #26842057 - 07/24/20 06:27 AM (3 years, 6 months ago)

It's funny that you mentioned 'canopy'. Before I edited my response to you I posted "nice grow, but have you got any canopies?".  I then chopped it to "nice grow" lol. I didn't want to come off as being arrogant. It seemed to me that all the ingredients for a canopy grow were there without the canopy (chuckle chuckle). I work in the health care business so I can first hand sympothise with your situation . What I can't do is spell sympethize, sympothize, sympeth??? Anyway, be cool and keep rockin' it:dancer:


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OfflineA.k.aM
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Registered: 10/27/19
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Re: Is this monoculture? [Re: scarabaeus]
    #26842141 - 07/24/20 07:43 AM (3 years, 6 months ago)

The whole thing has sectors, especially on the right side. If you’re gonna keep transferring trying to get a mono you should grow out every third plate or something and take pictures before harvest. It’s pretty cool how you can see the genetics get more and more uniform as you go.


--------------------
LAGM2020


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OfflineM.Dana
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Registered: 07/24/20
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Last seen: 3 years, 5 months
Re: Is this monoculture? [Re: A.k.a]
    #26842230 - 07/24/20 08:52 AM (3 years, 6 months ago)

Thanks guys, that was lots of great info.:heart:
Yes it's from a multispore transfer.


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OfflineM.Dana
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Registered: 07/24/20
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Re: Is this monoculture? [Re: AtmozFear]
    #26842231 - 07/24/20 08:53 AM (3 years, 6 months ago)

That makes sense, I guess I'll stick to this one then.


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Offlinerandomrad
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Registered: 05/28/20
Posts: 6
Last seen: 3 years, 4 months
Re: Is this monoculture? [Re: scarabaeus]
    #26842242 - 07/24/20 08:59 AM (3 years, 6 months ago)

Quote:

scarabaeus said:
You have an awesome rhizomorphic plate. This is a transfer from a multispore plate I'm guessing? Yes, at 2 O'clock there is a sector.  But it doesn't matter. No matter where you take wedges from to inoculate grains, that wedge will contain lots of strains. Even a tiny piece of your plate from anywhere on it will contain several if not many strains. Mycelium tends to sort itself out -compatible strains get together and stay together -they all behave similarly (as far as a grow is concerned). You don't want a single strain unless you are doing some serious (pro) lab work. Single strains are hard to maintain - they easily lose their vigor and die out.
A wedge that contains multiple strains that are compatible (like everything you have on your plate) means that genetic material is being exchanged between the various strains as they rub shoulders. This process is what keeps the culture vigorous and healthy. The job of mycelium is to eat, grow and mutate in an effort to stay alive. It needs compatible friends (strains) to do this.  An actual single strain is hung out to dry because it has no buns to bump with -a lonley place :begger:

Hope I'm not rambling. Hope I gave you sumpin' to think about :headbang:




Not rambling.  This is excellent information for me the newbie.  Thanks!


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OfflineAtmozFear
just a shade of myself
Male

Registered: 01/25/19
Posts: 1,032
Last seen: 1 day, 15 hours
Re: Is this monoculture? [Re: scarabaeus]
    #26842448 - 07/24/20 10:51 AM (3 years, 6 months ago)

Quote:

scarabaeus said:
It's funny that you mentioned 'canopy'. Before I edited my response to you I posted "nice grow, but have you got any canopies?".  I then chopped it to "nice grow" lol. I didn't want to come off as being arrogant. It seemed to me that all the ingredients for a canopy grow were there without the canopy (chuckle chuckle). I work in the health care business so I can first hand sympothise with your situation . What I can't do is spell sympethize, sympothize, sympeth??? Anyway, be cool and keep rockin' it:dancer:



thanks!


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