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OfflineYoyoma
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Registered: 07/24/18
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Help! Contams on edges!
    #26841369 - 07/23/20 07:28 PM (3 years, 6 months ago)



There's a small piece of contamination at the edge of each jar. One is slightly toughing the myc. My question is..

Do I transfer the contams away since it's so small?
Do I transfer the whole mycelium away for the same reason?
Or would I just transer small pieces as normal?

note: this is my first grow


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Offlinemeowjinx
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Re: Help! Contams on edges! [Re: Yoyoma]
    #26841399 - 07/23/20 07:41 PM (3 years, 6 months ago)

Hello

You don't "remove" contaminants out w/ agar. It's not like you're trying to save the agar dish. The point of agar is just to grow a healthy mycelium so that you can inoculate something else. When you see a contaminant on an agar dish it's best to just dump that dish after a transfer

Once you see a contaminant such as that mold you cut out a (preferably) small piece of mycelium to a new container. You don't need to transfer the whole thing. The more of it you take with you the higher the chance that you also take a contaminant w/ you to the next plate, I know because I've goofed and it's happened to me in the past


Edited by meowjinx (07/23/20 07:42 PM)


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OfflineYoyoma
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Re: Help! Contams on edges! [Re: meowjinx]
    #26841489 - 07/23/20 08:25 PM (3 years, 6 months ago)

Gotcha. Thanks! Transfer then toss. TTT lol

These were actually transfers themselves. What do I do when the myc has reached the end of the jar? (my original jar) Wait for it to pin? Inoculate?


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Offlinemeowjinx
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Re: Help! Contams on edges! [Re: Yoyoma]
    #26841536 - 07/23/20 08:51 PM (3 years, 6 months ago)

No problemo. Yeah, TTT hehe

"The end" as in it has colonized the whole jar? And "first" as in it was the one inoculated w/ a spore syringe?

If it was from spore syringe I would just toss it in case it had hidden contams. Green mold is super easy to spot, but not all contams give such obvious visual cues. But you can use it to inoculate a grain jar if you'd like. Or multiple grain jars. Only reason to try to have it pin would be if you were trying to create a clone or something, but I can't see why you'd wanna do that w/ your first plate

W/ agar jars/plates you basically have three options: use them (to inoculate something), dump them out, or refrigerate them for long-term storage

But like w/ cloning, storing in the fridge is mainly done when you want to hold on to some desirable trait ("good genetics"). Most people wouldn't do that w/ their first plate. But you CAN if you'd like


Edited by meowjinx (07/23/20 08:53 PM)


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OfflineYoyoma
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Re: Help! Contams on edges! [Re: meowjinx]
    #26841615 - 07/23/20 09:47 PM (3 years, 6 months ago)

Spore syringe, yes.
Although I do have two more transfers still growing with no contams so far that I can see.

I just heard that, if you wait to pin first you can guarantee that it will fruit.. not sure why it wouldn't fruit in the first place to be quite honest. And these are "1st generation" transfers, if you will. I should maybe transfer a couple more times before inoculating?





fyi: This original plate is interesting because it was the first or second plate that I ever inoculated that didn't contam out of like 16 lol. Pretty weird as my technique was definitely worse at first.


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