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Invisiblerickyswamps
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Re: Making LC cultivation lids - how and where to get the parts [Re: Blue Helix] * 1
    #26839850 - 07/23/20 06:26 AM (3 years, 6 months ago)

I quit using LC lids and just use my grain lids.  Do inoculation free pouring and it does great.  Unless there is a certain reason it has to go into a syringe.  Just another step and process to worry about.


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Offlinemushpunx
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Re: Making LC cultivation lids - how and where to get the parts [Re: Blue Helix]
    #26839862 - 07/23/20 06:41 AM (3 years, 6 months ago)

Quote:

Blue Helix said:
Quote:

mushpunx said:
Nice thread! I recently made some of these up to give a try. I used the "autoclave safe" plastic lids you can get off cultivation sites (I think I got mine off FP). I've given these a really good shaking with water in the jar and haven't found them to leak at all without having to use a silicone gasket.

I have some of the heavy duty rubber injection ports but I haven't set them in yet.


Have you ever had a contaminant introduced from pulling syringes up thru the injection port? Do you just sanitize it with ISO first?

Thanks!






I tend to fill the dimple on top of the injection port with a spray of ISO.  Then when the needle is going in, it passes through it.  When you remove it, it passes through it.  I've never had contamination spread via the route.




Thanks man! Maybe I'll try this then. Loading a number of syringes up sounds a lot easier this way.


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Offlinemushpunx
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Re: Making LC cultivation lids - how and where to get the parts [Re: rickyswamps]
    #26839866 - 07/23/20 06:51 AM (3 years, 6 months ago)

Quote:

rickyswamps said:
I quit using LC lids and just use my grain lids.  Do inoculation free pouring and it does great.  Unless there is a certain reason it has to go into a syringe.  Just another step and process to worry about.




I always just used my grain lids too, but after I had an accident when a shelf in the refrigerator fell I decided to give them a try for safer storage.
They're so cheap and easy I think I'm gonna stick with them.



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Invisiblesandman420
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Re: Making LC cultivation lids - how and where to get the parts [Re: mushpunx]
    #26839901 - 07/23/20 07:23 AM (3 years, 6 months ago)

Free pour just not really possible to inoculate spawn bags. The floppy top of the bag is too unwieldy.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed


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OfflineCamera93
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Re: Making LC cultivation lids - how and where to get the parts [Re: mushpunx]
    #26839905 - 07/23/20 07:30 AM (3 years, 6 months ago)

Quote:

mushpunx said:





I get confused around what this is?

On my lids i have a hole with sfd over it, and another hole i put one of those heavy duty rubber SHIPS. I got timid when I went to use it tho, as it seemed to require quite a bit of force to get the needle to pierce thru. I opted for opening the jar and at an angle aspirating to syringe. I really would rather do it with lid on the whole time lol. what does that syringe filter I quoted do? Does it act like my SFD or do you suck up the LC thru that?

an example of my lids:



--------------------
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Invisiblerickyswamps
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Re: Making LC cultivation lids - how and where to get the parts [Re: sandman420] * 1
    #26839963 - 07/23/20 08:20 AM (3 years, 6 months ago)

Quote:

sandman420 said:
Free pour just not really possible to inoculate spawn bags. The floppy top of the bag is too unwieldy.



Not really true.  Do it all the time and works fine.  Open the bag in front of the hood and pour in.  Bag will stand straight up if you folded it well before PCing.

Anyways, don't really want to derail the how to make a lid thread.  Just know that its an option that works.  :cool:


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InvisiblePastywhyteMDiscord
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Re: Making LC cultivation lids - how and where to get the parts [Re: rickyswamps]
    #26840006 - 07/23/20 08:43 AM (3 years, 6 months ago)

Quote:

rickyswamps said:
Quote:

sandman420 said:
Free pour just not really possible to inoculate spawn bags. The floppy top of the bag is too unwieldy.



Not really true.  Do it all the time and works fine.  Open the bag in front of the hood and pour in.  Bag will stand straight up if you folded it well before PCing.

Anyways, don't really want to derail the how to make a lid thread.  Just know that its an option that works.  :cool:




I agree. I’ve done free pour many times and it’s no more difficult than G2G is.


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Invisiblesandman420
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Re: Making LC cultivation lids - how and where to get the parts [Re: Pastywhyte]
    #26840009 - 07/23/20 08:44 AM (3 years, 6 months ago)

When I tried it the liquid hits the side of the bag and makes it want to fold over sometimes. It was a bit of a struggle to keep the bag open.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed


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OfflineBlue Helix
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Re: Making LC cultivation lids - how and where to get the parts [Re: sandman420]
    #26843896 - 07/25/20 01:28 AM (3 years, 6 months ago)

Quote:

sandman420 said:
Free pour just not really possible to inoculate spawn bags. The floppy top of the bag is too unwieldy.




Free pouring isn't, but the use of LCs into spawn bags is what I always have done.  Every single grow I have on this site was done via LC directly inoculating and injected into a spawn bag without any spawn ever having been created.  My substrates always include grain as a component, but I've never used grain jars - not once.  I've covered this topic many, many times, but basically, I just inject in a tap-reinforced part of the spawn bag and cover with hot glue.  It works PERFECTLY and you don't waste your time with spawn.



Again, this is how ALL my grows are done.  I've never touched spawn for 20 years.  Here are the typical results too:



How I am growing the mushrooms is easier than using agar and spawning.  The first time I inoculate LCs with a tiny bit of spores (the only time I ever use a SAB).  Ever after I don't touch the SAB or flow hoods or anything like that. Instead, everything is injection-based so how clean the room is doesn't matter.  All subsequent LCs are inoculated with a half or one ml of some stored LC.  LCs are stored in vacutainers which are like 15 cents each and last forever, so I don't need to go back to spores.  I don't see a lot of people doing what I do, but I cannot seem to change people's old habits even when they are harder.  Growers just seem to keep with the PK tek or agar and spawning for some strange reason.


Edited by Blue Helix (07/25/20 01:52 AM)


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Offlinemushpunx
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Re: Making LC cultivation lids - how and where to get the parts [Re: Camera93] * 1
    #26843953 - 07/25/20 03:18 AM (3 years, 6 months ago)

Quote:

Camera93 said:
Quote:

mushpunx said:





I get confused around what this is?

On my lids i have a hole with sfd over it, and another hole i put one of those heavy duty rubber SHIPS. I got timid when I went to use it tho, as it seemed to require quite a bit of force to get the needle to pierce thru. I opted for opening the jar and at an angle aspirating to syringe. I really would rather do it with lid on the whole time lol. what does that syringe filter I quoted do? Does it act like my SFD or do you suck up the LC thru that?

an example of my lids:






It's a syringe wheel filter, it has a micron filter inside. They are meant for gong on the end of syringes to pull liquid thru (it will filter out some bacteria, particlulates etc). We don't use them for that though, we repurpose them to use as GE filters.

It's just a GE filter that works very well with LC because it's spill proof. You wouldn't want to get an SFD or polyfil etc soaked with LC.
You don't aspirate through the syringe filter. I just started using these, I open the cap but you can add a rubber SHIP like OP did if you want.

They're only $1.00 or so. For the one I used I just drill a hole with a 5/32 drill bit and pop it thru,no silicone or anything. It's a good lid for LC.


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Invisiblesandman420
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Re: Making LC cultivation lids - how and where to get the parts [Re: mushpunx]
    #26843994 - 07/25/20 04:56 AM (3 years, 6 months ago)

LC rules and your tek is solid my man. Always been an inspiration to me Mr Helix.


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed


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InvisibleKernale
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Re: Making LC cultivation lids - how and where to get the parts [Re: Blue Helix]
    #26844889 - 07/25/20 04:27 PM (3 years, 6 months ago)

Quote:

Blue Helix said:
Quote:

sandman420 said:
Free pour just not really possible to inoculate spawn bags. The floppy top of the bag is too unwieldy.




Free pouring isn't, but the use of LCs into spawn bags is what I always have done.  Every single grow I have on this site was done via LC directly inoculating and injected into a spawn bag without any spawn ever having been created.  My substrates always include grain as a component, but I've never used grain jars - not once.  I've covered this topic many, many times, but basically, I just inject in a tap-reinforced part of the spawn bag and cover with hot glue.  It works PERFECTLY and you don't waste your time with spawn.



Again, this is how ALL my grows are done.  I've never touched spawn for 20 years.  Here are the typical results too:



How I am growing the mushrooms is easier than using agar and spawning.  The first time I inoculate LCs with a tiny bit of spores (the only time I ever use a SAB).  Ever after I don't touch the SAB or flow hoods or anything like that. Instead, everything is injection-based so how clean the room is doesn't matter.  All subsequent LCs are inoculated with a half or one ml of some stored LC.  LCs are stored in vacutainers which are like 15 cents each and last forever, so I don't need to go back to spores.  I don't see a lot of people doing what I do, but I cannot seem to change people's old habits even when they are harder.  Growers just seem to keep with the PK tek or agar and spawning for some strange reason.





That seems super convenient. After it all colonizes do you just break it up and put it in a tub? Do you have a substrate recipe? How have I never seen this style before...

I think this needs to be a tek!


--------------------


Edited by Kernale (07/25/20 04:28 PM)


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OfflineBlue Helix
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Re: Making LC cultivation lids - how and where to get the parts [Re: Kernale] * 3
    #26845427 - 07/26/20 01:58 AM (3 years, 6 months ago)

Quote:

sandman420 said:

That seems super convenient. After it all colonizes do you just break it up and put it in a tub? Do you have a substrate recipe? How have I never seen this style before...

I think this needs to be a tek!




I guess it sort of is a tek, but yeah, you can use LC to create spawn or, like I do, to just go directly to the sterilized bulk substrate.  I use a big ass 12-gauge needle and 140ml to inoculate, but that's not necessary; you could use a small 16-gauge needle dispensing only 60 ml too with probably the same results (hell you might be able to use even 20ml for all I know - never tried it).  When it comes time to lay the trays, I spread the colonized substrate out and even the top as much as I can with a baking roller (no pressure is applied though and you could do this with your hand probably about as well).  Sometimes I let it recolonize a little (I call it reconsolidation) by putting some saran wrap with toothpick holes over the tray for 1 to 4 days at fruiting temps.  I then apply the 50:50 casing around 1/4" for pan cyans (maybe a little deeper for cubes), and it's off to the fruiting chamber.  I set the fruiting temperature to 78F and the humidity to 100%.  The chamber air has very gentle circulation via two tiny USB clip fans I bought. The circulation should never be strong and never be directed forcefully directly at the tray surface.  If you can feel it above the tray with a dry hand, it's probably too much airflow.  I also mist the trays extra sometimes by hand or with a hand mister until I see pins.  Once the pins are about an inch or two, I drop the humidity to 90 to 95% to encourage them to reach full maturity nicely (that last little drop seems to help but many folks don't bother with it). 

If you search my alias "Blue Helix" you can find lots of grow logs on here as well as on Mycotopia (I think I actually have more grow logs on Mycotopia than here).  I tried all sorts of fancy substrates over the years, but I didn't find the fancy ones worked any better than the basics to be honest. In fact, it seemed to me that the more fancy they were, the worse they worked.  Anymore I always use the same simple substrate recipe because it gives me the best results of any of them:

Quote:

Manure-based Standard Substrate
  • Dehydrated Horse Manure 44 oz
  • Wild bird seed 10 oz
  • Vermiculite 1.5 L
  • Water       ~100 oz

Pressure cook for 4 to 5 hours.
Makes about 10 pounds total at 65%+/-1% moisture, but you can scale it down or up as you want.




Edited by Blue Helix (07/26/20 02:08 AM)


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InvisibleKernale
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Re: Making LC cultivation lids - how and where to get the parts [Re: Blue Helix]
    #26845988 - 07/26/20 11:44 AM (3 years, 6 months ago)

Im gonna have to try this out for sure. I hate PCing jars and love (clean) LC so this seems like my kind of method. Thanks for writing it down man! Ill have to take a gander at your growlogs too.


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OfflineBlue Helix
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Re: Making LC cultivation lids - how and where to get the parts [Re: Kernale] * 1
    #26854324 - 07/30/20 08:10 PM (3 years, 6 months ago)

Quote:

Kernale said:
Im gonna have to try this out for sure. I hate PCing jars and love (clean) LC so this seems like my kind of method. Thanks for writing it down man! Ill have to take a gander at your growlogs too.




If, after looking at my grow logs here and on Mycotopia, you still have any questions, please shoot my PM.  I promise you this: once you go LC direct to fruit-out substrate, you will never go back to spawn unless you are opening a big mushroom farm where it makes sense.


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InvisibleKernale
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Re: Making LC cultivation lids - how and where to get the parts [Re: Blue Helix]
    #26854639 - 07/30/20 11:45 PM (3 years, 6 months ago)

Hell yeah, thanks man.  :cheers:


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InvisiblecoAsTal
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Re: Making LC cultivation lids - how and where to get the parts [Re: Blue Helix]
    #26855312 - 07/31/20 11:07 AM (3 years, 6 months ago)

I took your advice on these parts and have ordered the components to get my LC game going-- I also like the vials as a long-term storage medium.

:threadmonitor:


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Offlinesenescence
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Re: Making LC cultivation lids - how and where to get the parts [Re: Blue Helix]
    #26855449 - 07/31/20 12:23 PM (3 years, 6 months ago)

Hey Blue, I just made a thread the other day where I was seeking out your LC-straight-to-Bulk TEK in particular. It's ahead of it's time for sure, can't figure out why more people aren't doing it. I'm going to be trying it this week by just free pouring some LC into the bags (no syringe for now), and I think I'm just going to cut the tops off of the bags once fully colonized (or maybe even remove the plastic completely like people do for Shiitake blocks) and move them into a dedicated fruiting room.

Quote:

Blue Helix said:
you will never go back to spawn unless you are opening a big mushroom farm where it makes sense.



Why does it make more sense to make spawn at a commercial op? I can't see how using LC to bulk at a big farm wouldn't also be super useful/competitive.


Edited by senescence (07/31/20 12:26 PM)


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OfflineBlue Helix
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Re: Making LC cultivation lids - how and where to get the parts [Re: coAsTal]
    #26855451 - 07/31/20 12:24 PM (3 years, 6 months ago)

Quote:

coAsTal said:
I took your advice on these parts and have ordered the components to get my LC game going-- I also like the vials as a long-term storage medium.

:threadmonitor:




You are going to love it!  You gotta drill two holes - that's it!  Assuming you are going to use the red septums from the vacutainers as an injection port (which you should use over the separate grey ones which are a lower quality), the two drill bit sizes for the two holes you need to drill are as follows:

Required Drill Bits for Above
Red silicone septum for injection port - 1/2"
Blue hydrophobic filters listed above  - 7/32"


For the half inch I use a diamond bell bit from Harbor Freight, but you could probably get away with using a normal drill bit if you take it slow (and if you are using a hand drill you can't really use those bells unless they have a center anchor bit which they usually don't for a half-inch one like that).  If you are using a hand drill rather than a table one, I'd recommend first drilling a much smaller hole, though, (say 1/8" or even the 7/32") to guide the 1/2" bit in so it doesn't jump (again, for the buck at least, Harbor Freight is your friend as long as you don't mind supporting the Chinese I guess).

Let me know if you have any other questions!


Edited by Blue Helix (07/31/20 12:41 PM)


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InvisiblecoAsTal
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Re: Making LC cultivation lids - how and where to get the parts [Re: Blue Helix]
    #26855481 - 07/31/20 12:41 PM (3 years, 6 months ago)

Many thanks for the advice-- I have both those sizes, but they're DeWalt standard bits-- I have many more of the lids coming than I will use for this project, so I expect no problems if I crack one or two.

Funny you mentioned the smaller pilot holes, because I was thinking that was going to be the approach I took as well. Super excited to get them all put together. I'll be careful-

You don't mention using any red sealant at the hole edges-- unless I missed it. I would like to make sure I have everything on hand when I put them together (probably sometime next weekend)


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I am certain of nothing but the holiness of the Heart's affections and the truth of Imagination--  John Keats

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