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AlpineHighs
Thrill Shrieker

Registered: 04/22/20
Posts: 87
Loc: Montucky
Last seen: 1 year, 6 months
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Re: Spore syringe ==> LME Agar Recipe? [Re: Shenmue]
#26802417 - 07/03/20 10:25 PM (3 years, 7 months ago) |
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Haha. Lucky man
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CrashTest
Stay Learning


Registered: 06/21/20
Posts: 623
Last seen: 7 months, 17 days
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Re: Spore syringe ==> LME Agar Recipe? [Re: Shenmue]
#26802721 - 07/04/20 02:47 AM (3 years, 7 months ago) |
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Quote:
Shenmue said:
Quote:
CrashTest said: Good looks on the pre made plates from Etsy, just grabbed 12 of them for 38$ shipped. Definitely pricey but I don’t have a PC yet and want to try agar ASAP.
Dude they're only 35$ for 20 on etsy with free shipping. You got ripped off.
First time looking for them and only one supplier popped up. I’m just gonna cancel that order and buy these lol
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this account is automated, any posts related to activities or advice thereof are strictly opinions from numerous online sites and are for informational purposes only - CrashTest2020
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CrashTest
Stay Learning


Registered: 06/21/20
Posts: 623
Last seen: 7 months, 17 days
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Re: Spore syringe ==> LME Agar Recipe? [Re: Shenmue]
#26802728 - 07/04/20 02:53 AM (3 years, 7 months ago) |
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--------------------
this account is automated, any posts related to activities or advice thereof are strictly opinions from numerous online sites and are for informational purposes only - CrashTest2020
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AlpineHighs
Thrill Shrieker

Registered: 04/22/20
Posts: 87
Loc: Montucky
Last seen: 1 year, 6 months
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Well, looks like another failed attempt at MSS to agar. I inoculated 10 plates with a Costa Rica syringe on 7/3. Only saw 2 tiny growths after ~10 days of what looked like mycelium, and transferred it to 4 new plates on 7/16.
Here's the status today...
   
Is that even mycelium? Is it worth continuing to transfer, or would I just be transferring contams?
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Gan
Wielder of Narya



Registered: 08/26/19
Posts: 927
Loc: Valinor
Last seen: 6 months, 15 days
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Re: Spore syringe ==> LME Agar Recipe? [Re: AlpineHighs]
#26839289 - 07/22/20 08:55 PM (3 years, 6 months ago) |
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It's questionable. What's the strength of your nutes in your agar? Also, do you have pics of your previous germination plates you took those transfers from?
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mushboy
modboy



Registered: 04/24/05
Posts: 32,391
Loc: where?
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Re: Spore syringe ==> LME Agar Recipe? [Re: AlpineHighs]
#26839306 - 07/22/20 08:59 PM (3 years, 6 months ago) |
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Quote:
AlpineHighs said: Is that even mycelium?
not cube thats for sure. i think thats toast howd your germ plate look?
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AlpineHighs
Thrill Shrieker

Registered: 04/22/20
Posts: 87
Loc: Montucky
Last seen: 1 year, 6 months
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Re: Spore syringe ==> LME Agar Recipe? [Re: Gan]
#26839308 - 07/22/20 09:00 PM (3 years, 6 months ago) |
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10g agar / 9g LME / 500ml H2O
Don't have pics of the 2 originals, but the two growths were weak, about the size of a nickle
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mushboy
modboy



Registered: 04/24/05
Posts: 32,391
Loc: where?
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Re: Spore syringe ==> LME Agar Recipe? [Re: AlpineHighs]
#26839309 - 07/22/20 09:01 PM (3 years, 6 months ago) |
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bummer try again
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AlpineHighs
Thrill Shrieker

Registered: 04/22/20
Posts: 87
Loc: Montucky
Last seen: 1 year, 6 months
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Re: Spore syringe ==> LME Agar Recipe? [Re: mushboy]
#26839311 - 07/22/20 09:03 PM (3 years, 6 months ago) |
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Dammit. Thanks
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Gan
Wielder of Narya



Registered: 08/26/19
Posts: 927
Loc: Valinor
Last seen: 6 months, 15 days
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Re: Spore syringe ==> LME Agar Recipe? [Re: AlpineHighs]
#26839435 - 07/22/20 10:19 PM (3 years, 6 months ago) |
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Just a tip that helped me out when I was starting. Keep your germ plate (or any plates you transferred from) for a few days, if you have the room. When I was new, a lot of times I thought I was transferring clean myc. But I'd look back at the donor plate and it would become obvious what was truly clean myc I grabbed and what was a kinda similar looking mold.
Definitely not necessary. Especially if you dont have the room. But it helped me for the first couple months to learn to spot healthy myc and contams. It was especially useful for building an eye for those pesky contams that are hidden within the myc.
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