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OfflineTotodile386
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I Want To Just Inject Straight To Monotub With Spore Solution/L.C.?
    #26837659 - 07/22/20 07:57 AM (3 years, 6 months ago)

Good day, all.

I don't understand why everyone goes, "NO U CANNOT INOCULATE A MONOTUB!" Why not?

"u cant inoculate a montuib cuz itsa take too long" -- It would take less time if I used more spore solution or L.C., right? 'Cause more of that means more early coverage, right?

"u can nt unoculate a monotubbs cause it will contam" -- If I make sure my work environment is disinfected, I'm clean, the monotub was sterilized, and the substrate was added hot, I don't see how there's more of a risk for contamination.

"wat exactly r u gona do?" -- I want to take a ready-to-grow monotub of substrate, stick a disinfected spore syringe or L.C. syringe through the stuffing, and just spurt spores onto the substrate generously and let it grow.

"u dont know tha first thang about grain spawn..." -- I have done P.F. technique enough to know what's going on.

Are not 2 sparrows sold for a penny? And outdoor mushrooms grow and thrive without any sterilization, nor grain-spawn. Plus, open-air indoor grow kits, w.t.f. then? And consider even the P.F. tek; P.F. tek mushrooms don't bulk-a-tize or store away in grain-spawn, yet our Heavenly Creator definitely causes mushrooms to sprout out from these jars.

Why are you saying it's impossible to inject straight to monotub?


Edited by Totodile386 (07/22/20 07:59 AM)


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OfflineAtmozFear
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Re: I Want To Just Inject Straight To Monotub With Spore Solution/L.C.? [Re: Totodile386]
    #26837665 - 07/22/20 08:02 AM (3 years, 6 months ago)

super obvious reasons, the biggest one is that it is simply not enough mycelium. 

But- someone did try an experiment with that a while ago, but I never followed up.


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OfflineTotodile386
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Re: I Want To Just Inject Straight To Monotub With Spore Solution/L.C.? [Re: AtmozFear]
    #26837677 - 07/22/20 08:11 AM (3 years, 6 months ago)

Quote:

AtmozFear said:
super obvious reasons, the biggest one is that it is simply not enough mycelium. 

But- someone did try an experiment with that a while ago, but I never followed up.




I remember from the P.F. technique, it said:

"When you inoculate your jar, less spore solution is more. If you use more, more mycelium will spawn, but it won't really accelerate this process."

Therefore, I thought a whole monotub can be colonized by applying generously spore solution onto its substrate, why not.

If you could find that experiment you said, that might be helpful.


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OfflineB21
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Re: I Want To Just Inject Straight To Monotub With Spore Solution/L.C.? [Re: Totodile386]
    #26837685 - 07/22/20 08:17 AM (3 years, 6 months ago)

Will definitely not work. I do not want to down your idea...this is friendly advice,,, just do it the right way. Follow a proven tek to the T.
Monotubs and pf cakes are totally different.
Monotub substrate should not be sterilized.
If by some miracle it does work you’ll end up.....wait a minute!! It’s not gonna work


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OfflineB21
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Re: I Want To Just Inject Straight To Monotub With Spore Solution/L.C.? [Re: B21]
    #26837686 - 07/22/20 08:18 AM (3 years, 6 months ago)

And YES YOU CAN INOCULATE A MONOTUB....with GRAIN SPAWN


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OfflineItsaMeShroomio
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Re: I Want To Just Inject Straight To Monotub With Spore Solution/L.C.? [Re: Totodile386]
    #26837689 - 07/22/20 08:20 AM (3 years, 6 months ago)

I think contamination is the main problem. If you can seal your monotub contam-tight, pressure cook it, aseptically inoculate then reseal it air-tight, that will probably work, but I'm guessing that's not an option. But merely putting hot substrate and injecting through monotub polyfil is unlikely to result in anything close to sterility. The uncolonized grains in the substrate are ripe for colonization by contams. It's certainly possible that you can make it work if you use a large amount of clean LC, but its much more likely you are going to grow lots of mold.


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InvisibleAnastomosis
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Re: I Want To Just Inject Straight To Monotub With Spore Solution/L.C.? [Re: Totodile386]
    #26837692 - 07/22/20 08:24 AM (3 years, 6 months ago)



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OfflineEl Chupacabra
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Re: I Want To Just Inject Straight To Monotub With Spore Solution/L.C.? [Re: Anastomosis]
    #26837695 - 07/22/20 08:25 AM (3 years, 6 months ago)

I think the better question is why, why would you want to attempt this? Even if this were to have good results what's the point in trying to do it this way? Spores are too precious and not to mention dirty to try something like this.
To me it's almost like asking if it's possible to skin an animal only using sticks.

Quote:

If I make sure my work environment is disinfected, I'm clean, the monotub was sterilized, and the substrate was added hot, I don't see how there's more of a risk for contamination



Quote:

I have done P.F. technique enough to know what's going on


.

You may have technique but you clearly don't have the research. If I were u I would just follow popular teks and keep the good results.


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OfflineTotodile386
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Re: I Want To Just Inject Straight To Monotub With Spore Solution/L.C.? [Re: El Chupacabra]
    #26837708 - 07/22/20 08:41 AM (3 years, 6 months ago)

It's simple. Spores or L.C. land on substrate and proceed to bring forth mycelia.

Mycelium eats its way through all it can, covering the substrate throughout.

Mycelium consolidates together, turning itself into a cake of substrate energy.

The mycelium cake realizes it's out of space, and sends up fruiting bodies up and to where light is.

Fruiting bodies grow and are either collected or drop their spores and die.

Infection is merely an invasive foreign presence that landed on the friendly substrate.

What don't I get?


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Offlinejcm4620
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Re: I Want To Just Inject Straight To Monotub With Spore Solution/L.C.? [Re: El Chupacabra] * 2
    #26837711 - 07/22/20 08:42 AM (3 years, 6 months ago)

inoc a monotub with spores lol thats the dumbest thing i think ive heard in a long time and belive me we see some real stupid shit.

the damn thing would contam out before it would even come close to being colonized plus its take a good god damn long time if it would even work at all.

use agar to grain to bulk like a normal person would 🤷🏼


even using LC is a stupid idea.


idk yall i smell a troll here


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Edited by jcm4620 (07/22/20 08:43 AM)


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OfflineMarxcelium
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Re: I Want To Just Inject Straight To Monotub With Spore Solution/L.C.? [Re: Totodile386]
    #26837714 - 07/22/20 08:45 AM (3 years, 6 months ago)

I love your posts, Totodile, and, frankly, I'd say don't let anyone discourage you from following your dreams. There's definitely not decades of experience and data from cultivators across this site that your idea completely ignores.

No criticism formed against your wonderful plan shall prosper, as the good book might put it. I'd go so far as to say this may be divinely inspired, and it would be a crime against heaven for you not to try this and see what happens.


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OfflineEl Chupacabra
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Re: I Want To Just Inject Straight To Monotub With Spore Solution/L.C.? [Re: Totodile386]
    #26837715 - 07/22/20 08:46 AM (3 years, 6 months ago)

Quote:

Totodile386 said:
It's simple. Spores or L.C. land on substrate and proceed to bring forth mycelia.

Mycelium eats its way through all it can, covering the substrate throughout.

Mycelium consolidates together, turning itself into a cake of substrate energy.

The mycelium cake realizes it's out of space, and sends up fruiting bodies up and to where light is.

Fruiting bodies grow and are either collected or drop their spores and die.

Infection is merely an invasive foreign presence that landed on the friendly substrate.

What don't I get?




Oh I get it You've ran this simulation through your head and in your head you see results.. The thing is is if you attempt that in reality the mushroom gods won't really give a fuck about your simulation.


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OfflineTotodile386
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Re: I Want To Just Inject Straight To Monotub With Spore Solution/L.C.? [Re: jcm4620]
    #26837720 - 07/22/20 08:48 AM (3 years, 6 months ago)

Quote:

jcm4620 said:
inoc a monotub with spores lol thats the dumbest thing i think ive heard in a long time and belive me we see some real stupid shit.

the damn thing would contam out before it would even come close to being colonized plus its take a good god damn long time if it would even work at all.

use agar to grain to bulk like a normal person would 🤷🏼




Again, no basis of these accusations..

Like I said, using more solution should cover the substrate more rapidly, before an infection can work its way in.

What is this? David and Goliath?

"You come against me with mold spores and clubs, but I come to you in the Name of the LORD, Elohim Who-Suffices, the God of Israel.

This day the LORD will deliver you into my hands, and I'll strike you down and cut off your head. This very day I will give the carcasses of the infection army to the birds and the wild animals, and the whole world will know that there is a God in Israel.

All those gathered here will know that it is not by sword or spear that the LORD saves; for the battle is the LORD's, and One will give all of you into our hands."


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OfflineAtmozFear
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Re: I Want To Just Inject Straight To Monotub With Spore Solution/L.C.? [Re: jcm4620]
    #26837723 - 07/22/20 08:50 AM (3 years, 6 months ago)

i agree.  pretty dumb.  Most starting out come up with all this ingenious shit and they eventually get good... this guy's going reverse into retardation.


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Edited by AtmozFear (07/22/20 08:51 AM)


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OfflineRoscoeReturnsS
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Re: I Want To Just Inject Straight To Monotub With Spore Solution/L.C.? [Re: Marxcelium]
    #26837726 - 07/22/20 08:51 AM (3 years, 6 months ago)

You absolutely can inoculate a mono. Your results may not be what you are hoping to achieve. If you think it’s a better way to grow, please start a grow log and show us how it works. I know I’d love to follow along.


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Re: I Want To Just Inject Straight To Monotub With Spore Solution/L.C.? [Re: Totodile386]
    #26837728 - 07/22/20 08:52 AM (3 years, 6 months ago)

I swear I knew some bible shit was coming after saying the idea was sum dumb shit. :lol:


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OfflineAtmozFear
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Re: I Want To Just Inject Straight To Monotub With Spore Solution/L.C.? [Re: RoscoeReturns]
    #26837730 - 07/22/20 08:53 AM (3 years, 6 months ago)

Quote:

RoscoeReturns said:
You absolutely can inoculate a mono. Your results may not be what you are hoping to achieve. If you think it’s a better way to grow, please start a grow log and show us how it works. I know I’d love to follow along.



roscoe- don't.


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OfflineMarxcelium
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Re: I Want To Just Inject Straight To Monotub With Spore Solution/L.C.? [Re: El Chupacabra] * 1
    #26837732 - 07/22/20 08:54 AM (3 years, 6 months ago)

Quote:

El Chupacabra said:
The thing is is if you attempt that in reality the mushroom gods won't really give a fuck about your simulation.




But you see, the one true God will bend the mushroom gods to his will.  This is Totodile TEK.


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OfflineEl Chupacabra
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Re: I Want To Just Inject Straight To Monotub With Spore Solution/L.C.? [Re: Marxcelium]
    #26837733 - 07/22/20 08:56 AM (3 years, 6 months ago)

A truly divine being sent here to teach us all to grow drugs sounds truly inspirational.

You guys are the best!


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OfflineTotodile386
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Re: I Want To Just Inject Straight To Monotub With Spore Solution/L.C.? [Re: RoscoeReturns]
    #26837740 - 07/22/20 09:01 AM (3 years, 6 months ago)

Quote:

RoscoeReturns said:
You absolutely can inoculate a mono. Your results may not be what you are hoping to achieve. If you think it’s a better way to grow, please start a grow log and show us how it works. I know I’d love to follow along.




This.

Even if my brand new straight-to-monotub method (I'm gonna try enoki first time) flops in some way, it would mean it was not God's will for me to have it or for it to happen.

I have heard the people's arguments against straight-to-monotub already, and I'm not afraid, but faithfully optimistic.


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OfflineAtmozFear
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Re: I Want To Just Inject Straight To Monotub With Spore Solution/L.C.? [Re: Totodile386]
    #26837741 - 07/22/20 09:02 AM (3 years, 6 months ago)

you're annoying as shit


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InvisibleRoger Clemency
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Re: I Want To Just Inject Straight To Monotub With Spore Solution/L.C.? [Re: Marxcelium]
    #26837743 - 07/22/20 09:03 AM (3 years, 6 months ago)

The grain spawn is what supplies nutrition. If you squirted a bunch of LC in a mono full of bulk sub you might get it to fully colonize, probably you’d have to load up your tub with dry coir and completely get it to field capacity with LC...which is a lot. Then if it did colonize you’d get a very small yield. I don’t know this for sure but I’ve got some experience growing and someone did do a test with just a jar of coir and a bit of LC recently, he got a few little mushrooms out of it but nothing to speak of compared to using even a bit of grain in there.

If you try inoculating it with spores they just won’t germinate.


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Sour grapes, sweet revenge
Heaven starts right where hell ends


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OfflineTotodile386
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Re: I Want To Just Inject Straight To Monotub With Spore Solution/L.C.? [Re: AtmozFear]
    #26837748 - 07/22/20 09:04 AM (3 years, 6 months ago)

Quote:

AtmozFear said:
you're annoying as shit




I'm no holy-see, but maybe your outlook has part to do with it. Treat thy neighbor as yourself.


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OfflineTotodile386
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Re: I Want To Just Inject Straight To Monotub With Spore Solution/L.C.? [Re: Roger Clemency]
    #26837754 - 07/22/20 09:06 AM (3 years, 6 months ago)

Quote:

Roger Clemency said:
The grain spawn is what supplies nutrition. If you squirted a bunch of LC in a mono full of bulk sub you might get it to fully colonize, probably you’d have to load up your tub with dry coir and completely get it to field capacity with LC...which is a lot. Then if it did colonize you’d get a very small yield. I don’t know this for sure but I’ve got some experience growing and someone did do a test with just a jar of coir and a bit of LC recently, he got a few little mushrooms out of it but nothing to speak of compared to using even a bit of grain in there.

If you try inoculating it with spores they just won’t germinate.




The straight-to-monotub substrate will be simple: just organic coco coir, organic sawdust, and organic rye flour in a ratio of something like 1:2:1, and cooked just before adding to monotub in a U.V. and I.P.A. sterilized space.


Edited by Totodile386 (07/22/20 09:07 AM)


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OfflineAtmozFear
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Re: I Want To Just Inject Straight To Monotub With Spore Solution/L.C.? [Re: Roger Clemency]
    #26837760 - 07/22/20 09:08 AM (3 years, 6 months ago)

Quote:

Roger Clemency said:
someone did do a test with just a jar of coir and a bit of LC recently



you remember that?  I think Mateah was following along with it intensely but can't remember whose thread it was.


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Re: I Want To Just Inject Straight To Monotub With Spore Solution/L.C.? [Re: AtmozFear]
    #26837774 - 07/22/20 09:15 AM (3 years, 6 months ago)

If you want to do allinone things you can try muda bottles.

I think a monotub is just too much surface and substrate, maybe you can make a tub of mudabottles? xd


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Offlinejcm4620
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Re: I Want To Just Inject Straight To Monotub With Spore Solution/L.C.? [Re: 0ptiquest] * 1
    #26837780 - 07/22/20 09:17 AM (3 years, 6 months ago)

kinda funny how he took the i smell a troll part out of my quote huh


dont get baited into it yall hes an idiot. just use ur time here to read shit worth reading


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PANAEOLUS FRUITING MADE SIMPLE



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InvisibleRoger Clemency
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Re: I Want To Just Inject Straight To Monotub With Spore Solution/L.C.? [Re: AtmozFear]
    #26837782 - 07/22/20 09:19 AM (3 years, 6 months ago)

It was Vladimir Putin in a face shield. Can’t remember the guys name on here but he’s a badass.

Totodile - sounds sketchy contamination wise with the rye flour but if you’re careful it may work. It’s just that there are much much easier ways to grow cubes but if you’re intent on skipping PC cycles? then give it a try. Talking about it will just bring you round in circles.

Lol jcm. They always know exactly what riles people up, a lot of these hit several major sticking points to make sure people will jump in like “wtf no NOOO” But I like to engage sometimes for future readers or on the off chance of seriousness.


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Sour grapes, sweet revenge
Heaven starts right where hell ends


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OfflineGan
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Re: I Want To Just Inject Straight To Monotub With Spore Solution/L.C.? [Re: Totodile386]
    #26837783 - 07/22/20 09:20 AM (3 years, 6 months ago)

Quote:

Totodile386 said:
Quote:

RoscoeReturns said:
You absolutely can inoculate a mono. Your results may not be what you are hoping to achieve. If you think it’s a better way to grow, please start a grow log and show us how it works. I know I’d love to follow along.




This.

Even if my brand new straight-to-monotub method (I'm gonna try enoki first time) flops in some way, it would mean it was not God's will for me to have it or for it to happen.

I have heard the people's arguments against straight-to-monotub already, and I'm not afraid, but faithfully optimistic.




That's like a doctor performing major heart surgery with non-sterilized tools in the middle of a nasty, moldy barn then the patient dying from massive infections and the doctor saying "well it wasn't in God's will for him to live." In reality, he just used the wrong tools that have been proven to work then blamed the shortcomings on God's will.

There's methods that have been proven to work over countless attempts that people are suggesting. If you search around you'll see that a syringe to straight monotub would more than likely contam the fuck out pretty quick at the least.


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OfflineEl Chupacabra
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Re: I Want To Just Inject Straight To Monotub With Spore Solution/L.C.? [Re: Gan]
    #26837789 - 07/22/20 09:26 AM (3 years, 6 months ago)

I would love to see this thread die because it really is pointless. Pointless that OP decided to even post, pointless that anyone decided to reply and that includes myself.. if OP wants to do this then why not just create a thread of you actually doing this. If you want to post nonsense and stir shit up and get people heated then stay out of the cultivation forums unless you're actually cultivating something more than a half ass idea.

I'm really not trying to be rude but it's really hard trying to sound nice when this is all so ridiculous.


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OfflineMarxcelium
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Re: I Want To Just Inject Straight To Monotub With Spore Solution/L.C.? [Re: Roger Clemency]
    #26837805 - 07/22/20 09:34 AM (3 years, 6 months ago)

Roger, yeah I was gonna say, that was c10's experiment. Can't find the original post now, though.:confused:


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Re: I Want To Just Inject Straight To Monotub With Spore Solution/L.C.? [Re: Marxcelium] * 3
    #26837853 - 07/22/20 09:54 AM (3 years, 6 months ago)

I love when people who understand next to nothing about the organism they are attempting to propagate then attempt to innovate. I myself have done many things that were not exactly “on tek.” But I had done years of actual study to allow me to pull off such outlandish projects.

You can’t sterilize a monotub unless you have access to very special equipment. At any rate you won’t be able to keep it sterile unless you have a fucking flowhood the size of a wall or a clean room. Sterile isn’t just wiping something down with chemicals. Spores are also most certainly not axenic and all syringes should be expected to have some CFU’s present.

To anyone who understands these things it’s all very clear why you can’t inoculate a monotub. The OP’s question reads like “do I need to jack up the car to change the tire? Can’t I just lift it up with one hand and change the tire with the other?”


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Offlinejcm4620
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Re: I Want To Just Inject Straight To Monotub With Spore Solution/L.C.? [Re: Pastywhyte] * 1
    #26837869 - 07/22/20 09:59 AM (3 years, 6 months ago)

Quote:

Pastywhyte said:
I love when people who understand next to nothing about the organism they are attempting to propagate then attempt to innovate. I myself have done many things that were not exactly “on tek.” But I had done years of actual study to allow me to pull off such outlandish projects.

You can’t sterilize a monotub unless you have access to very special equipment. At any rate you won’t be able to keep it sterile unless you have a fucking flowhood the size of a wall or a clean room. Sterile isn’t just wiping something down with chemicals. Spores are also most certainly not axenic and all syringes should be expected to have some CFU’s present.

To anyone who understands these things it’s all very clear why you can’t inoculate a monotub. The OP’s question reads like “do I need to jack up the car to change the tire? Can’t I just lift it up with one hand and change the tire with the other?”





dont u know pasty most young men today cant even change a tire 😂😂😂😂


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Re: I Want To Just Inject Straight To Monotub With Spore Solution/L.C.? [Re: jcm4620]
    #26837872 - 07/22/20 10:00 AM (3 years, 6 months ago)

That’s pathetic.


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InvisiblePastywhyteMDiscord
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Re: I Want To Just Inject Straight To Monotub With Spore Solution/L.C.? [Re: Pastywhyte]
    #26837877 - 07/22/20 10:02 AM (3 years, 6 months ago)



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Offlinejcm4620
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Re: I Want To Just Inject Straight To Monotub With Spore Solution/L.C.? [Re: jcm4620]
    #26837878 - 07/22/20 10:03 AM (3 years, 6 months ago)

lol cant change a tire and cant tell time on a hand clock lol smh. my buds wife is a highschool teacher here and she said that they had to come in and change all the clocks to digital cuz not 1 kid could read a hand clock. i know this is off topic but seriously wtf so with the amount of education ppl get these days its no wonder he thinks this way


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Offlineuncle_curly
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Re: I Want To Just Inject Straight To Monotub With Spore Solution/L.C.? [Re: Pastywhyte]
    #26837884 - 07/22/20 10:04 AM (3 years, 6 months ago)

My experience is that the rich substrate will attract endless contaminates and it will get stinky and weird colored.  However, if you let the mycelium fill the tub, it makes a really good start to an outdoor grow if you have a suitable spot.  The contaminates will get consumed by stuff in the soil, cover with verm and keep damp but not soaked.


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Re: I Want To Just Inject Straight To Monotub With Spore Solution/L.C.? [Re: Totodile386] * 1
    #26837890 - 07/22/20 10:09 AM (3 years, 6 months ago)

This is some quality trolling in Jesus name amen


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OfflineEl Chupacabra
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Re: I Want To Just Inject Straight To Monotub With Spore Solution/L.C.? [Re: Gastronomicus]
    #26837898 - 07/22/20 10:12 AM (3 years, 6 months ago)

Maybe, just maybe if the spore solution has electrolytes in it. Hmmm.

Go away I'm bating!

(Last post in this thread I swear, I'm out!)


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InvisiblecoAsTal
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Re: I Want To Just Inject Straight To Monotub With Spore Solution/L.C.? [Re: Totodile386]
    #26837949 - 07/22/20 10:33 AM (3 years, 6 months ago)

Quote:

Totodile386 said:

What is this? David and Goliath?

"You come against me with mold spores and clubs, but I come to you in the Name of the LORD, Elohim Who-Suffices, the God of Israel.

This day the LORD will deliver you into my hands, and I'll strike you down and cut off your head. This very day I will give the carcasses of the infection army to the birds and the wild animals, and the whole world will know that there is a God in Israel.

All those gathered here will know that it is not by sword or spear that the LORD saves; for the battle is the LORD's, and One will give all of you into our hands."





Idiot fucking troll.

:permabanned:


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Re: I Want To Just Inject Straight To Monotub With Spore Solution/L.C.? [Re: Totodile386]
    #26837992 - 07/22/20 10:33 AM (3 years, 6 months ago)

This thread has been closed.

Reason:
This is degenerating into something which has zero value to the cultivation community. I’m locking it before tempers flair and the trolling gets too out of hand.


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