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funchky
Determined
Registered: 04/19/20
Posts: 56
Last seen: 3 years, 4 months
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Re: Panaeolus Grow Log Report [Re: McGrimm]
#26877895 - 08/13/20 05:16 AM (3 years, 5 months ago) |
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You are an inspiration BH. Thank you for taking the time to put together the detail on your fruiting set up. I'm going to redesign my Martha with some of this in mind. I think excessive airflow drawing downward caused the last 2 total failures. Not to knock other teks because it obviously works brilliantly for some but I just couldn't get it dialed in right. Casing stayed moist but the subs were turning blue on the sides. No fruits at all. And thank you for the recommendation on Stamet's book. I've already ready through a good portion of it.
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Celestialexplorer1



Registered: 03/25/20
Posts: 1,803
Loc: Floating in a tin can
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Re: Panaeolus Grow Log Report [Re: funchky]
#26878579 - 08/13/20 01:34 PM (3 years, 5 months ago) |
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Hey so I have a question in the log it sounded like you said you had used multispore LC, and by that I’m just assuming you meant multicultural LC or multiple sectors and if so I’m just curious how you usually go about making the multi cultural LC. Like do you just find several good sectors and put them all together on a plate before transferring to LC?
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 To spend just one moment in eternity
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Blue Helix
bold hand


Registered: 02/02/03
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Last seen: 6 months, 18 days
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Quote:
Celestialexplorer1 said: Hey so I have a question in the log it sounded like you said you had used multispore LC, and by that I’m just assuming you meant multicultural LC or multiple sectors and if so I’m just curious how you usually go about making the multi cultural LC. Like do you just find several good sectors and put them all together on a plate before transferring to LC?
I usually put some spores on an agar surface and sector the margin. I do not sector down further because no one has demonstrated to me the usefulness of doing that. jcm4620 tried with a slightly more aggressive version of the cambos, but I didn't find it performed any better in the long run. He'd say that is because he took prints of ones that were already strong, and maybe he's right. However, until just 5 years ago, I never had a problem with multispore ALL THE TIME. I've been doing multispore since day one, and I will switch once someone can demonstrate to me a higher biological efficiency can be attained via a clone. No one has taken me up on that challenge yet, though.
Likewise, I don't even believe that a mutispore will perpetuate a bad sector provided you didn't go past T1 or maybe T2. In other words, the bad sectors are a result of doing agar work. As soon as you do that, you start to mess up the naturally strong genes of the species. There is an idea floating around that because of the agar stuff, we've domesticated pan cyans to be weak. The obvious question is, then, why would you bother if multispore is so strong and you are messing up the species?
I should note that my views are only my own. The popular notion of growing pan cyan and cambo involves sectoring to about T4 and then growing over and over until you get one as strong as a multispore. In other words, the popular notion is that all growers should be waist deep in agar plates and spend most of their time growing on agar, not in substrates. I guess it's just a different approach. I like harvesting better, and others strongly prefer looking at agar plates. There is nothing wrong with either thing in my mind.
Edited by Blue Helix (08/13/20 07:11 PM)
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Wall.E
Bacteria's Bitch



Registered: 06/05/20
Posts: 2,860
Loc: Fungal Void
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Re: Panaeolus Grow Log Report [Re: Blue Helix]
#26879074 - 08/13/20 06:42 PM (3 years, 5 months ago) |
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Thanks for your response yesterday bh
-------------------- Life’s shit, but I’m loving it
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coAsTal
Friend


Registered: 04/04/06
Posts: 2,970
Loc: 8a
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Re: Panaeolus Grow Log Report [Re: Blue Helix]
#26879090 - 08/13/20 06:55 PM (3 years, 5 months ago) |
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You still haven't told us about your $12 stirrer, mister...
-------------------- I am certain of nothing but the holiness of the Heart's affections and the truth of Imagination-- John Keats Spore Trading List
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Blue Helix
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Re: Panaeolus Grow Log Report [Re: coAsTal] 1
#26879102 - 08/13/20 07:04 PM (3 years, 5 months ago) |
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Quote:
coAsTal said: You still haven't told us about your $12 stirrer, mister...

I guess it's more like $20 unless you want a "quick stirring one." I can make a quick stirring one (1000 RPM) with a PC fan, two magnets, and a small 9V DC power supply for less than $10. The problem is that I don't think that is a very good one, so I don't want to explain it to you.
Now if you want a $20 awesome one that will last you a lifetime and actually stirs at the right speed, then I made a new thread:
https://www.shroomery.org/forums/showflat.php/Number/26877787
Of course, source your power supply on eBay for $2 (saves you $5 over the exact same one through Amazon). And you can find two powerful magnets on eBay for less than 50 cents I'm sure.
Edited by Blue Helix (08/13/20 07:18 PM)
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Celestialexplorer1



Registered: 03/25/20
Posts: 1,803
Loc: Floating in a tin can
Last seen: 2 months, 12 days
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Re: Panaeolus Grow Log Report [Re: Blue Helix]
#26879261 - 08/13/20 09:22 PM (3 years, 5 months ago) |
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I actually happen to have all the materials for this stirrer and to make it battery powered for two aaa batteries. Gonna whip one up one day soon
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 To spend just one moment in eternity
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Celestialexplorer1



Registered: 03/25/20
Posts: 1,803
Loc: Floating in a tin can
Last seen: 2 months, 12 days
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Thank you for that answer as well. Just with that answer( which I have been suspecting is true for a long long time but never actually heard anyone say it) I’m going to start doing the same thing from now on.
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 To spend just one moment in eternity
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
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I totally agree with you on isolating. I use mostly t1 or t2 for everything.
The more you isolate the more extreme your results should be which can go either way.
I ran a PE clone a while back where I used a wedge from the tissue plate and then one from a t3 and the difference was huge. The tissue plate grew like an ms grow while the t3 was wall to wall similar shrooms.
In this case the t3 was much better but I’ve also had it go the other way where I was keeping shitty genes.
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LAGM2020     
Edited by A.k.a (08/14/20 07:17 AM)
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gt40
I will proof smthng



Registered: 09/17/12
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Re: Panaeolus Grow Log Report [Re: A.k.a]
#26879749 - 08/14/20 08:37 AM (3 years, 5 months ago) |
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Blue Helix is it safe to use such a powerful ozonizer if you have wedges & jars with live cultures in adjoining closed room?
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jcm4620
Stranger


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Re: Panaeolus Grow Log Report [Re: A.k.a]
#26879750 - 08/14/20 08:37 AM (3 years, 5 months ago) |
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yup thats exactly why i put that in my write up also. cuz if ur just sitting there playin with agar eventually your just gnna be transfering away from what u may want. i strictly am only trying to get a clean strong culture to get on my grain as fast as possible. i never go past t2 or t3 from a spore plate. just cuz it looks pretty and evenly round dont mean shit. im after clones anyway so i want a nice wide range of genetics to choose from. now once i get a clone il transfer it down and try to get the very best out of it that i can but thats something completely different.
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Celestialexplorer1



Registered: 03/25/20
Posts: 1,803
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Re: Panaeolus Grow Log Report [Re: jcm4620]
#26879778 - 08/14/20 09:05 AM (3 years, 5 months ago) |
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I already did it to a degree but I’ve always taken the smallest transfers of the best looking sectors to about t2 or 3 which basically lead to isolation or close to it with how small my transfers are. Example
this is a T2 I let the T1 grow out a bit before transfer though
 This is a T3 Both are cubes But you see how quick near isolation can be sometimes. Also it seems like the germination rate for spores might be a little lower with pans as apposed to cubes. So isolation inevitably would be faster as well.
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 To spend just one moment in eternity
Edited by Celestialexplorer1 (08/14/20 09:08 AM)
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Blue Helix
bold hand


Registered: 02/02/03
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Re: Panaeolus Grow Log Report [Re: gt40]
#26880210 - 08/14/20 02:02 PM (3 years, 5 months ago) |
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Quote:
gt40 said: Blue Helix is it safe to use such a powerful ozonizer if you have wedges & jars with live cultures in adjoining closed room?
I always have the windows open and run the HEPA in the room which has a carbon that neutralizes ozone fast. I only ozone the chamber empty, but no, if the ozone touched the bags, plates, or spores, it would kill them. You can smell it very strongly (well before it's toxic), so I just make sure I don't gas myself and everyone else in the place... lol It all dissipates pretty fast.
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Blue Helix
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Re: Panaeolus Grow Log Report [Re: jcm4620]
#26880224 - 08/14/20 02:11 PM (3 years, 5 months ago) |
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Quote:
jcm4620 said: yup thats exactly why i put that in my write up also. cuz if ur just sitting there playin with agar eventually your just gnna be transfering away from what u may want. i strictly am only trying to get a clean strong culture to get on my grain as fast as possible. i never go past t2 or t3 from a spore plate. just cuz it looks pretty and evenly round dont mean shit. im after clones anyway so i want a nice wide range of genetics to choose from. now once i get a clone il transfer it down and try to get the very best out of it that i can but thats something completely different.
Once you get that culture, you stick with it I bet. If I have a good run, I always keep the LC vacutainer for the same reason. It can take awhile to get a decent run, and when you do, you want to make sure you have a way back to it. I just like getting away from spores ASAP with this stuff.
I hate sounding like this old cranky man, but it does seem that back in the day, there weren't so many problems. Or maybe I just didn't grow as much as my memory thinks. Whatever it was, seems that MS used to work better, or my memory works worse (probably the latter).
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Celestialexplorer1



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Posts: 1,803
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Re: Panaeolus Grow Log Report [Re: Blue Helix]
#26880421 - 08/14/20 04:40 PM (3 years, 5 months ago) |
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The only truly successful pan grow I’ve had where I actually had a good flush was my first grow and surprisingly the only multispore grow I did of pans ever.
 Nothing special by any means but hey I can’t say it was a failure either. This was multispore to grain to coir/Verm with 10% manure. The substrate was meant for cubes and used accidentally because it was my first pan grow and I simply grabbed the wrong bag. But it worked.
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 To spend just one moment in eternity
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Celestialexplorer1



Registered: 03/25/20
Posts: 1,803
Loc: Floating in a tin can
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Ever since then I’ve moved everything to agar and have been having a lot of trouble even getting a flush in most cases but I think there was a lot of other things in play, other kinks I’ve been working out
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 To spend just one moment in eternity
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Asura
Cyantist


Registered: 08/01/11
Posts: 5,047
Loc: Texas
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My grow didn't go as well as you guys, but I am not disappointed at all. I think these are about the easiest pan I have ever grown and I am sure I could get better runs out of these.
  
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Wall.E
Bacteria's Bitch



Registered: 06/05/20
Posts: 2,860
Loc: Fungal Void
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Re: Panaeolus Grow Log Report [Re: Asura]
#26884538 - 08/17/20 09:36 AM (3 years, 5 months ago) |
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Still a pretty tray. Love the caps of those guys
-------------------- Life’s shit, but I’m loving it
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A.k.a
Stranger



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Re: Panaeolus Grow Log Report [Re: Wall.E]
#26884550 - 08/17/20 09:44 AM (3 years, 5 months ago) |
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These are the cambo jam right??
I wonder why mine had such different caps. I guess it’s a pheno thing but i haven’t seen it anywhere else. Mine were much smaller and flatter than normal.
 
They never got that half circle domed look.
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LAGM2020     
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Asura
Cyantist


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Loc: Texas
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Re: Panaeolus Grow Log Report [Re: A.k.a]
#26884558 - 08/17/20 09:47 AM (3 years, 5 months ago) |
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I never would have known these were cambo if I hadn't seen the grows here and started with a culture. Looks like cyans to me. The only clue is the caps and stipe are a little thicker.
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