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OfflineCodeMath
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Registered: 06/16/20
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Rotting Smell in Rye Grain Bags?
    #26832085 - 07/19/20 07:43 AM (3 years, 6 months ago)

So I have around 10 bags of rye grain that I have inoculated, some on 6/27 and the rest on 7/6. 

Unfortunately, for reasons I have yet to understand, the ones that are colonizing are colonizing quite slowly, but look good.  However, I have a few bags that just never started colonizing.  They were in the same group as others that are currently colonizing but never showed growth?  There are also no visible contaminants growing either. 

A couple of them are starting to smell on the inside.  Rotting rye grain.  Should I just throw these or is there any hope for colonization left?  It's been about 3 weeks.


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Offlinejcm4620
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Registered: 05/26/19
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Re: Rotting Smell in Rye Grain Bags? [Re: CodeMath]
    #26832120 - 07/19/20 08:13 AM (3 years, 6 months ago)

well if they smell rotten toss em out they shouldnt smell like that at all. did u make the bags urself or buy em ?? did u knock up with a spore syringe??

im guessin from just readin that u used a spore syringe to grain which can work and i have done it plenty of times but its more risky is all. your best best is to knock up via an agar wedge or a LC. spore syringes are never 100% clean far far from it. heres a good example of why you use agar. this shitty bacteria came from a syringe but luckily i was able to clean it up away from it.



also if you bought premade bags your adding in more of a risk for contams. just like everything else in life if u want it done right do it yourself😃 prints are way better than syringes also


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Offlineredhandmat
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Registered: 05/09/19
Posts: 463
Last seen: 2 years, 8 months
Re: Rotting Smell in Rye Grain Bags? [Re: CodeMath]
    #26832124 - 07/19/20 08:15 AM (3 years, 6 months ago)

I work in jars, not bags, but full colonization happens within 2 weeks. I cant imagine 3 weeks and no growth at all in bags. You haven't told us how you inoculated them? Vendor syringe, or did you clean on agar, make a new syringe? or something else?

If it smells bad it could be improper sterilization to begin with, dirty syringes, a combination of both, or faults when inoculating.

Could we have pictures?


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InvisibleLotKid
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Registered: 01/07/17
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Re: Rotting Smell in Rye Grain Bags? [Re: redhandmat]
    #26832163 - 07/19/20 08:48 AM (3 years, 6 months ago)

Rotten smell is usually a bacillus infection. It's also called "wet spot" or "sour rot"... it stinks.

Once you see or smell any jars or bags that have a bacillus infection... throw them away. There's no turning them around.


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