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capz
Mysterious Stranger


Registered: 07/18/20
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Cloudy Orange Liquid - Grain Jars
#26830662 - 07/18/20 12:11 PM (3 years, 6 months ago) |
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Hello Everyone,
New to the site, and seeking some information.
I’ve searched the site to the best of my abilities, reviews the contam post and have not seen anything quite like my situation, and not have much exp, I am unable to identify what this is. Please refer to the photo attached.
There are grain spawn jars, and are about 3 weeks a long, but several of them have encountered this orangey cloudy liquid, and I don’t believe it’s metabolites, but if it is, what could be causing this excess amount?
Any information would be much appreciated!
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Dr. Whitethumb
Pumperfunkfunkerpump



Registered: 06/21/20
Posts: 42
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Re: Cloudy Orange Liquid - Grain Jars [Re: capz]
#26830691 - 07/18/20 12:27 PM (3 years, 6 months ago) |
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Quote:
capz said: There are grain spawn jars, and are about 3 weeks a long, but several of them have encountered this orangey cloudy liquid, and I don’t believe it’s metabolites, but if it is, what could be causing this excess amount?
Welcome dude, I think it's bacterial contamination. I'm not sure what spp. of bacteria is causing the orange goop, but for future reference, metabolites will appear to be a translucent-yellow color. Kinda like poorly-hydrated pee pressed up in between your myc and jar
-------------------- “No one is born hating another person because of the color of his skin, or his background, or his religion. People must learn to hate, and if they can learn to hate, they can be taught to love, for love comes more naturally to the human heart than its opposite.” - Nelson Mandela
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Roger Clemency
Smile


Registered: 03/23/20
Posts: 2,005
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Re: Cloudy Orange Liquid - Grain Jars [Re: capz]
#26830694 - 07/18/20 12:30 PM (3 years, 6 months ago) |
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Some type of bacteria, friend. Possibly from going spore syringe straight to grain?
-------------------- Sour grapes, sweet revenge Heaven starts right where hell ends
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Martinsapin
no-mad



Registered: 09/12/16
Posts: 310
Loc: Canada
Last seen: 1 year, 7 months
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if it smells bad toss it
grains seems too wet.
let it dry/drain longer before putting em in jars.
my rule of thumb is thats grains/substrate are better to be on the dry side than too wet side, of course the best is to do it right. but too wet bring contams everytime for me
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capz
Mysterious Stranger


Registered: 07/18/20
Posts: 15
Last seen: 1 year, 7 months
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Re: Cloudy Orange Liquid - Grain Jars [Re: capz]
#26831499 - 07/18/20 08:53 PM (3 years, 6 months ago) |
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Thank you all for the prompt replies and insight.
Looks like I will be tossing a few of my jars, unfortunately.
Would it be recommended to add verm to my grain jars to help with this? Also should the verm be a field capacity when putting in?
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ManifoldPrime
Per Ardua Ad Astra



Registered: 03/16/17
Posts: 1,313
Loc: South Africa
Last seen: 1 year, 1 month
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Re: Cloudy Orange Liquid - Grain Jars [Re: capz]
#26831943 - 07/19/20 05:06 AM (3 years, 6 months ago) |
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If you are using a spore syringe, the verm wont stop bacteria from a syringe. Your best bet is to put your remaining syringe to agar, and clean up the culture first. https://www.shroomery.org/forums/showflat.php/Number/21922023 https://www.shroomery.org/forums/showflat.php/Number/24330957/fpart/all/vc/1
If you inocculated from agar, then either your culture was bad, your transfer procedure was sloppy, your grain prep was insufficient, or your filter on your jar failed. Based on where the bacteria is (bottom of jar) it is either liquid innoculant thats bad or the grain was bad. Filter fails and transfer sterile procedure fails usually contaminate on top.
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Edited by ManifoldPrime (07/19/20 05:07 AM)
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capz
Mysterious Stranger


Registered: 07/18/20
Posts: 15
Last seen: 1 year, 7 months
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Re: Cloudy Orange Liquid - Grain Jars [Re: ManifoldPrime]
#26833051 - 07/19/20 06:51 PM (3 years, 6 months ago) |
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Thanks for the resources to review.
Well, unfortunately the syring was used up, across several jars. It appears only 2 out the batch took without whatever this contam is taking hold. I will research this Agar process more throughly.
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Roger Clemency
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Registered: 03/23/20
Posts: 2,005
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Re: Cloudy Orange Liquid - Grain Jars [Re: capz]
#26833069 - 07/19/20 07:10 PM (3 years, 6 months ago) |
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Yeah man agar is step #1 if you're using grains. Spores straight to grain can definitely work but there is often going to be other stuff in that syringe besides cube spores.
Agar allows you to actually germinate your spores so you can see what you've got, so if there's cube myc and some mold and bacteria all growing from your print/syringe you can cut a little wedge of just the cube myc and transfer that to another agar plate, and you can see it grow out and know if it's clean or not. Inoculating with spores is like shooting in the dark.
-------------------- Sour grapes, sweet revenge Heaven starts right where hell ends
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