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InvisibleTheDoobskerS
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Started from heavy bacterial contamination, now we're here
    #26830642 - 07/18/20 11:58 AM (3 years, 6 months ago)

So I posted about this plate earlier and got some good tips form you guys
It was taken from spores from these Nepal Chitwan cakes




Here I am 3 transfers later, and they're looking pretty clean to me. One of these does look a bit shiny around the edges, but I think it's water that fell onto it when I put a hot cup on top to get rid of condensation for the picture. (hoping so)




They came from these plates but from when they were much less grown out. I'm assuming that orange-like tint to the middle of this plate is bacteria that the myc was able to outgrow.
These 3 wedges on the outside were taken a couple days ago, and I'm hoping to use those 3 plates to put some wedges into grain jars.




Wanted some input/confirmation. Do you guys see any signs of contam still being present? Or would you say I could let them grow out another week and use as inoculant?


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InvisiblemushboyMDiscord
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Registered: 04/24/05
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Re: Started from heavy bacterial contamination, now we're here [Re: TheDoobsker]
    #26830670 - 07/18/20 12:14 PM (3 years, 6 months ago)

Imo that's got meshed in mold like a mofo. I wouldnt use that at all. I'd toss and try again tbh:shrug:

I dont think bacteria is present at all. Just mold. I would of tossed the original germ plate. If the spores are really important try streaking them with a loop or sterile swab to spread out the germination. It's easier to grab clean colonies from dirty spores.


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InvisibleRoger Clemency
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Registered: 03/23/20
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Re: Started from heavy bacterial contamination, now we're here [Re: mushboy]
    #26830679 - 07/18/20 12:18 PM (3 years, 6 months ago)

Yeah all 3 of those look really sketchy. The orange you're referring to in the donor plates is just where the myc has broken down the food coloring.

Did you take any clones from those mush or were you not set up with plates yet?


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InvisibleTheDoobskerS
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Registered: 05/22/20
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Re: Started from heavy bacterial contamination, now we're here [Re: Roger Clemency]
    #26830700 - 07/18/20 12:34 PM (3 years, 6 months ago)

Well shit

Yeah these may be my last shot at keeping these genetics. I got all my agar equipment just in time to not have any more fruits to clone.

I do still have the spore print, but I'm not expecting much out of it. I tried doing a streak and just got a bunch of bacteria. My loop was pretty shitty, I didn't do the best job streaking it, and I only did 1 plate.
I now have a good inoculation loop which I was planning to streak 5 new plates with today from this print. That will probably be the end of it's usable life, so if those don't work, I'll lose the strain.

I see where you arrived at the mold conclusion. Lot's of fuzziness everywhere, right? Is there no shot at cleaning it up?


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