|
ouuwee
Garden of Weeden



Registered: 03/22/20
Posts: 297
Last seen: 20 days, 7 hours
|
Re: Invisible looking ring around mycelium [Re: Marxcelium]
#26836316 - 07/21/20 03:18 PM (3 years, 6 months ago) |
|
|
I don't think we're talking about the same invisible ring. This is how mine looks after growing it out a bit. These were transfers on 7/15.
How it looks at first glance. Polypropylene plates so visibility isn't crystal clear. Also used activated carbon in an attempt to make black agar but instead I got greyish agar with sediments.

How it looks with proper lighting. You can see that mycelium tried to grow on plate 1, but after a while, the invisible ring takes over and mycelium growth stops completely. The third one you can see some sectoring
Edited by ouuwee (07/21/20 03:21 PM)
|
mushboy
modboy



Registered: 04/24/05
Posts: 32,274
Loc: where?
|
Re: Invisible looking ring around mycelium [Re: ouuwee]
#26836360 - 07/21/20 03:33 PM (3 years, 6 months ago) |
|
|
What's the strenght are those plates? Sorry if you already stated.
|
ouuwee
Garden of Weeden



Registered: 03/22/20
Posts: 297
Last seen: 20 days, 7 hours
|
Re: Invisible looking ring around mycelium [Re: mushboy]
#26836561 - 07/21/20 04:49 PM (3 years, 6 months ago) |
|
|
Quote:
mushboy said: What's the strenght are those plates? Sorry if you already stated.
What's the strength? Sorry not understanding your question
|
mushboy
modboy



Registered: 04/24/05
Posts: 32,274
Loc: where?
|
Re: Invisible looking ring around mycelium [Re: ouuwee]
#26836574 - 07/21/20 04:56 PM (3 years, 6 months ago) |
|
|
What % of nutrition per 1000/500 ml?
Asking because the few times I used shitty premixed agar I grew lots of these.
|
ouuwee
Garden of Weeden



Registered: 03/22/20
Posts: 297
Last seen: 20 days, 7 hours
|
Re: Invisible looking ring around mycelium [Re: mushboy]
#26836697 - 07/21/20 05:52 PM (3 years, 6 months ago) |
|
|
1.4% nutrition, 500 ml 7g agar 7g LME
I didn't use any premixes and I use the same ingredients that made perfectly fine plates before.
|
cowcatcher
Strange



Registered: 07/21/18
Posts: 30
Last seen: 16 days, 12 hours
|
Re: Invisible looking ring around mycelium [Re: ouuwee]
#26837669 - 07/22/20 08:04 AM (3 years, 6 months ago) |
|
|
I agree totally different than what I have. the ring I have is always about 2mm to 3mm in front of the leading edge of the mycelium and looks like transparent rhizomes.
 This is the best pic I could get of what i'm seeing.
--------------------
Micro dosing my blues away
|
mushboy
modboy



Registered: 04/24/05
Posts: 32,274
Loc: where?
|
Re: Invisible looking ring around mycelium [Re: cowcatcher] 1
#26837682 - 07/22/20 08:15 AM (3 years, 6 months ago) |
|
|
Take cuts from that bruh
If you looked at that under a scope I'm 99% positive it would be clean myc. Some cultures show it some dont ime.
|
Marxcelium
Mushroom Instrumentality Project

Registered: 05/12/20
Posts: 127
Loc: The Tharsis Bulge
Last seen: 2 years, 1 month
|
Re: Invisible looking ring around mycelium [Re: cowcatcher]
#26837736 - 07/22/20 08:58 AM (3 years, 6 months ago) |
|
|
Yeah, agreed as well. My situation more closely resembles this one than the OP's.
|
ItsaMeShroomio
Shell Parkour Artist

Registered: 05/27/20
Posts: 69
Last seen: 2 years, 9 months
|
Re: Invisible looking ring around mycelium [Re: Marxcelium]
#26839972 - 07/23/20 08:25 AM (3 years, 6 months ago) |
|
|
Running a test on transferring the irregular halo I see on some APE plates.
It definitely looks different then the very regular circular halo at the border of healthy growth (like cowcatchers pic):

In particular, it seems to slow down the growth and there are a few places where rhizomorphic mycelium seems to be trying to jump over the halo.
I transferred a bit from the halo region to LME agar and rye soak water agar, and in two days there is a noticeable transparent ring on the LME agar

But no growth yet (beyond the transferred LME wedge) on the rye soak water agar.

I have used the same batch of LME and rye soak water plates for healthy cube myc and it tends to grow at the same rate on both plates, so I highly suspect this is not healthy cube myc.
Will update with new pics when they grow out a bit more (and maybe I'll be brave enough to give them a sniff too).
|
dfwerydfhg
Stranger
Registered: 05/04/20
Posts: 194
|
Re: Invisible looking ring around mycelium [Re: ItsaMeShroomio]
#26855097 - 07/31/20 08:49 AM (3 years, 5 months ago) |
|
|
So I made one little change to my agar recipe, and the halos I've been getting* seem to have abruptly disappeared. Every transfer or clone plate I've made on this batch (about 10) has been halo-free. I've also seen more rhizo morphology than I'm used to- including in transfers from tomentose plates with a halo.
So unless this is a giant coincidence, I think the food coloring that I skipped this time was the culprit. Sadly the tubes don't say the brand, but it was some basic supermarket brand, probably McCormick. A "gel color", whatever that means. I only used the blue and green stuff.
*mine are the seemingly benign halos, no with apparent effect on growth speed or general quality, except for being associated with or causing tomentose growth
|
mycologi.st
Stranger
Registered: 07/15/20
Posts: 8
Last seen: 3 years, 2 months
|
Re: Invisible looking ring around mycelium [Re: dfwerydfhg]
#26923296 - 09/07/20 03:50 PM (3 years, 4 months ago) |
|
|
Quote:
dfwerydfhg said: So I made one little change to my agar recipe
Would you mind sharing what your change was?
|
jay.ach
Traveler



Registered: 05/25/19
Posts: 531
Loc: USDA Zone 8
Last seen: 52 minutes, 5 seconds
|
Re: Invisible looking ring around mycelium [Re: mycologi.st]
#26923426 - 09/07/20 05:23 PM (3 years, 4 months ago) |
|
|
Quote:
mycologi.st said:
Quote:
dfwerydfhg said: So I made one little change to my agar recipe
Would you mind sharing what your change was?
Sounds like he skipped adding the food coloring...
-------------------- “One's destination is never a place, but a new way of seeing things.” - Henry Miller LAGM v. 2.023 LAGM2021
|
|