Home | Community | Message Board

Magic-Mushrooms-Shop.com
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   Kraken Kratom Red Vein Kratom

Jump to first unread post Pages: < Back | 1 | 2  [ show all ]
Offlineouuwee
Garden of Weeden
 User Gallery


Registered: 03/22/20
Posts: 297
Last seen: 20 days, 7 hours
Re: Invisible looking ring around mycelium [Re: Marxcelium]
    #26836316 - 07/21/20 03:18 PM (3 years, 6 months ago)

I don't think we're talking about the same invisible ring. This is how mine looks after growing it out a bit. These were transfers on 7/15.

How it looks at first glance. Polypropylene plates so visibility isn't crystal clear. Also used activated carbon in an attempt to make black agar but instead I got greyish agar with sediments.


How it looks with proper lighting. You can see that mycelium tried to grow on plate 1, but after a while, the invisible ring takes over and mycelium growth stops completely. The third one you can see some sectoring




Edited by ouuwee (07/21/20 03:21 PM)


Extras: Filter Print Post Top
InvisiblemushboyMDiscord
modboy
 User Gallery


Registered: 04/24/05
Posts: 32,274
Loc: where?
Trusted Cultivator
OG Cultivator
Re: Invisible looking ring around mycelium [Re: ouuwee]
    #26836360 - 07/21/20 03:33 PM (3 years, 6 months ago)

What's the strenght are those plates? Sorry if you already stated.


Extras: Filter Print Post Top
Offlineouuwee
Garden of Weeden
 User Gallery


Registered: 03/22/20
Posts: 297
Last seen: 20 days, 7 hours
Re: Invisible looking ring around mycelium [Re: mushboy]
    #26836561 - 07/21/20 04:49 PM (3 years, 6 months ago)

Quote:

mushboy said:
What's the strenght are those plates? Sorry if you already stated.




What's the strength? Sorry not understanding your question


Extras: Filter Print Post Top
InvisiblemushboyMDiscord
modboy
 User Gallery


Registered: 04/24/05
Posts: 32,274
Loc: where?
Trusted Cultivator
OG Cultivator
Re: Invisible looking ring around mycelium [Re: ouuwee]
    #26836574 - 07/21/20 04:56 PM (3 years, 6 months ago)

What % of nutrition per 1000/500 ml?

Asking because the few times I used shitty premixed agar I grew lots of these.


Extras: Filter Print Post Top
Offlineouuwee
Garden of Weeden
 User Gallery


Registered: 03/22/20
Posts: 297
Last seen: 20 days, 7 hours
Re: Invisible looking ring around mycelium [Re: mushboy]
    #26836697 - 07/21/20 05:52 PM (3 years, 6 months ago)

1.4% nutrition, 500 ml 7g agar 7g LME

I didn't use any premixes and I use the same ingredients that made perfectly fine plates before.


Extras: Filter Print Post Top
Offlinecowcatcher
Strange
Male User Gallery


Registered: 07/21/18
Posts: 30
Last seen: 16 days, 12 hours
Re: Invisible looking ring around mycelium [Re: ouuwee]
    #26837669 - 07/22/20 08:04 AM (3 years, 6 months ago)

I agree totally different than what I have.
the ring I have is always about 2mm to 3mm in front of the leading edge of the mycelium and looks like transparent rhizomes.

This is the best pic I could get of what i'm seeing.


--------------------
:mushroom2:Micro dosing my blues away:mushroom2:


Extras: Filter Print Post Top
InvisiblemushboyMDiscord
modboy
 User Gallery


Registered: 04/24/05
Posts: 32,274
Loc: where?
Trusted Cultivator
OG Cultivator
Re: Invisible looking ring around mycelium [Re: cowcatcher] * 1
    #26837682 - 07/22/20 08:15 AM (3 years, 6 months ago)

Take cuts from that bruh:awesomenod:

If you looked at that under a scope I'm 99% positive it would be clean myc. Some cultures show it some dont ime.


Extras: Filter Print Post Top
OfflineMarxcelium
Mushroom Instrumentality Project

Registered: 05/12/20
Posts: 127
Loc: The Tharsis Bulge
Last seen: 2 years, 1 month
Re: Invisible looking ring around mycelium [Re: cowcatcher]
    #26837736 - 07/22/20 08:58 AM (3 years, 6 months ago)

Yeah, agreed as well.  My situation more closely resembles this one than the OP's.


Extras: Filter Print Post Top
OfflineItsaMeShroomio
Shell Parkour Artist
 User Gallery
Registered: 05/27/20
Posts: 69
Last seen: 2 years, 9 months
Re: Invisible looking ring around mycelium [Re: Marxcelium]
    #26839972 - 07/23/20 08:25 AM (3 years, 6 months ago)

Running a test on transferring the irregular halo I see on some APE plates.

It definitely looks different then the very regular circular halo at the border of healthy growth (like cowcatchers pic):



In particular, it seems to slow down the growth and there are a few places where rhizomorphic mycelium seems to be trying to jump over the halo.

I transferred a bit from the halo region to LME agar and rye soak water agar, and in two days there is a noticeable transparent ring on the LME agar



But no growth yet (beyond the transferred LME wedge) on the rye soak water agar.



I have used the same batch of LME and rye soak water plates for healthy cube myc and it tends to grow at the same rate on both plates, so I highly suspect this is not healthy cube myc.

Will update with new pics when they grow out a bit more (and maybe I'll be brave enough to give them a sniff too).


Extras: Filter Print Post Top
Invisibledfwerydfhg
Stranger
Registered: 05/04/20
Posts: 194
Re: Invisible looking ring around mycelium [Re: ItsaMeShroomio]
    #26855097 - 07/31/20 08:49 AM (3 years, 5 months ago)

So I made one little change to my agar recipe, and the halos I've been getting* seem to have abruptly disappeared. Every transfer or clone plate I've made on this batch (about 10) has been halo-free. I've also seen more rhizo morphology than I'm used to- including in transfers from tomentose plates with a halo.

So unless this is a giant coincidence, I think the food coloring that I skipped this time was the culprit. Sadly the tubes don't say the brand, but it was some basic supermarket brand, probably McCormick. A "gel color", whatever that means. I only used the blue and green stuff.

*mine are the seemingly benign halos, no with apparent effect on growth speed or general quality, except for being associated with or causing tomentose growth


Extras: Filter Print Post Top
Offlinemycologi.st
Stranger
Registered: 07/15/20
Posts: 8
Last seen: 3 years, 2 months
Re: Invisible looking ring around mycelium [Re: dfwerydfhg]
    #26923296 - 09/07/20 03:50 PM (3 years, 4 months ago)

Quote:

dfwerydfhg said:
So I made one little change to my agar recipe




Would you mind sharing what your change was?


Extras: Filter Print Post Top
Offlinejay.achS
Traveler
Male User Gallery


Registered: 05/25/19
Posts: 531
Loc: USDA Zone 8
Last seen: 52 minutes, 5 seconds
Re: Invisible looking ring around mycelium [Re: mycologi.st]
    #26923426 - 09/07/20 05:23 PM (3 years, 4 months ago)

Quote:

mycologi.st said:
Quote:

dfwerydfhg said:
So I made one little change to my agar recipe





Would you mind sharing what your change was?




Sounds like he skipped adding the food coloring...


--------------------
“One's destination is never a place, but a new way of seeing things.”  - Henry Miller

LAGM v. 2.023
LAGM2021


Extras: Filter Print Post Top
Jump to top Pages: < Back | 1 | 2  [ show all ]

Shop: Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   Kraken Kratom Red Vein Kratom


Similar ThreadsPosterViewsRepliesLast post
* How to make agar 2 agar transfer? esp 6,652 7 01/08/04 11:08 AM
by YidakiMan
* How does mycelium colonize substrate? Fd3000 8,143 5 07/10/02 05:47 AM
by Mycelium
* needle guage for sucking up mycelium... Mycelium 3,132 2 07/05/02 05:58 PM
by SixTango
* Is psylocybe in mycelium?
( 1 2 all )
Only1AK 18,217 23 01/28/21 05:07 PM
by falconman
* Agar, strange mycelium mantasman 154 2 09/07/20 08:54 AM
by mantasman
* G2G transfers
( 1 2 all )
kjb1891 32,777 20 12/19/18 11:45 AM
by Nichrome
* G2G Transfer? lvleph 4,456 9 11/20/02 12:28 PM
by scifi5001
* substrate transfer?!?! dets 2,910 8 11/13/02 12:29 AM
by SixTango

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
2,475 topic views. 24 members, 206 guests and 42 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.027 seconds spending 0.008 seconds on 12 queries.