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randomrad
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B+ Agar Contamination
#26830413 - 07/18/20 09:22 AM (3 years, 6 months ago) |
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Question for the agar experts. This is my first try and I have what looks like a contamination on my B+ plates. I transferred mycelium from several different plates to these second generation plates and there are two growth patterns. I'm hoping this is how B+ grows on agar. But I fear contamination. They all look like this.

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southbounpachyderm
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Re: B+ Agar Contamination [Re: randomrad]
#26830445 - 07/18/20 09:34 AM (3 years, 6 months ago) |
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Your fear is unfortunately correct. Those are both bacterial Luckily you can still transfer away from it. Take the very leading edge of your rhizo growth. You want to get the smallest possible transfer you can don't cut too deep into the growth just the outside edge.
Edit: I did not see the cobweb. That is a total loss unfortunately
Edited by southbounpachyderm (07/18/20 09:35 AM)
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Roger Clemency
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Registered: 03/23/20
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You do have cobweb it looks like all throughout that plate. There is nice cube myc though and you may be able to transfer that away and clean it up.
I'd start from spores again but also I'd take a tiny wedge of one of those ropes of cube myc and grow it out, I think you could clean that up with a couple transfers if you're careful.
-------------------- Sour grapes, sweet revenge Heaven starts right where hell ends
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southbounpachyderm
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Quote:
Roger Clemency said: You do have cobweb it looks like all throughout that plate. There is nice cube myc though and you may be able to transfer that away and clean it up.
I'd start from spores again but also I'd take a tiny wedge of one of those ropes of cube myc and grow it out, I think you could clean that up with a couple transfers if you're careful.
You don't think the cobweb would travel with it? it seems to be completely surrounding the myc.
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randomrad
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Boo. Thank you for your help!
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Roger Clemency
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Re: B+ Agar Contamination [Re: randomrad]
#26830616 - 07/18/20 11:35 AM (3 years, 6 months ago) |
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Yeah it will almost certainly come along with the first transfer but then you just need to find a spot where the myc grows outside of the mold.
If that is cobweb it's super fast so it might be a tough thing but I think if you cut long and thin along a rope and transfer you might get some clean myc to pop out somewhere on the next plate that you could grab.
That stuff might be all over the previously clean areas of the plate without actually being entangled with the cube myc.
-------------------- Sour grapes, sweet revenge Heaven starts right where hell ends
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southbounpachyderm
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Registered: 07/20/18
Posts: 282
Last seen: 3 years, 6 months
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Quote:
Roger Clemency said: Yeah it will almost certainly come along with the first transfer but then you just need to find a spot where the myc grows outside of the mold.
If that is cobweb it's super fast so it might be a tough thing but I think if you cut long and thin along a rope and transfer you might get some clean myc to pop out somewhere on the next plate that you could grab.
That stuff might be all over the previously clean areas of the plate without actually being entangled with the cube myc.
I would think the cobweb would take off much faster than the myc but I suppose anything is worth a shot!
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