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chopstick
nobody



Registered: 07/26/08
Posts: 5,268
Loc: Chin's Wok
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If you want to guaranteed beat trich, get a flowhood and isolate clean cultures on agar first before transferring to your master grain jars. You will probably also need to bleach bomb your lab if not your whole house. Chances are your using a contaminated print or syringe and the trich was present in your spawn the whole time.
Seriously. Use agar.
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ConcreteWaves



Registered: 01/08/13
Posts: 648
Loc: United States
Last seen: 2 years, 1 month
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Re: f**kin trich, every time!!! [Re: chopstick]
#18414801 - 06/13/13 05:15 PM (11 years, 7 months ago) |
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Quote:
chopstick said: If you want to guaranteed beat trich, get a flowhood and isolate clean cultures on agar first before transferring to your master grain jars.
Seriously. Use agar.
>.> Explain what this stuff is. I'm in an apartment, im not getting a flowhood, i know what this is from when i used to work in restaurants, but, these terms ive always wanted to know, what is isolate? isolate clean culture? Agar? I hear it's like jelly u buy from the korean store...idk how to use this at all. Help me out?
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chopstick
nobody



Registered: 07/26/08
Posts: 5,268
Loc: Chin's Wok
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Agar is seaweed extract which is used to solidify media. You can use 1/4 pint jars or petri dishes to make agar cultures. Spores germinate on agar, then you can transfer clean mycelium away from contamination to fresh agar plates to get a clean, contaminant-free culture, which you then transfer directly to grain, then g2g with confidence.
You will need to build a glovebox (its cheap) and you're going to want to bleach bomb that lab room to kill the millions of latent trich spores which you have undoubtedly propogated. Use a 1:10 ratio bleach/water and leave the room for 1-2 days after application
Look on here for teks involving agar, that is about as far as I can help you. Once you've started with agar it's not that hard to get the hang of it. Good luck.
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ConcreteWaves



Registered: 01/08/13
Posts: 648
Loc: United States
Last seen: 2 years, 1 month
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Re: f**kin trich, every time!!! [Re: chopstick]
#18415992 - 06/13/13 08:50 PM (11 years, 7 months ago) |
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u said it's used to solidify media. What does this mean? >.> Ill look it up too, but if u got the quick answer.
Someone told me, if u wanna clone a rlly nice shroom u got, cut the stem in half, and take a tiny little centimeter chunk from the middle of the stem, and put it on agar.
Question is...it grows on the agar? Or does it colonize the agar? so when u put it into a jar with grain, u put the whole agar and myc? Or the culture persay slides off the agar into the grain jar?
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cronicr



Registered: 08/07/11
Posts: 62,904
Loc: Van Isle
Last seen: 2 minutes, 50 seconds
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it colonizes the agar,and you just cut wedges out
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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ConcreteWaves



Registered: 01/08/13
Posts: 648
Loc: United States
Last seen: 2 years, 1 month
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Re: f**kin trich, every time!!! [Re: cronicr]
#18418851 - 06/14/13 01:12 PM (11 years, 7 months ago) |
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So whats better about agar grain masters > regular g2g xfer? Does is colonize faster on agar? How is it cleaner....people say it's clean xfer every time...but cant airborn bacterias get on your agar soon as your about to xfer? then get into your fresh grains....the wedge might be resistant to contam, but the fresh sterilized grain isn't.
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cronicr



Registered: 08/07/11
Posts: 62,904
Loc: Van Isle
Last seen: 2 minutes, 50 seconds
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Because you can spot contams on agar, and when you do wedges u use a sab gb or fh
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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ConcreteWaves



Registered: 01/08/13
Posts: 648
Loc: United States
Last seen: 2 years, 1 month
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Re: f**kin trich, every time!!! [Re: cronicr]
#18419315 - 06/14/13 03:19 PM (11 years, 7 months ago) |
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Quote:
cronicr said: and when you do wedges u use a sab gb or fh
sab gb or fh? >.>
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,870
Last seen: 2 months, 8 days
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Quote:
ConcreteWaves said: So whats better about agar grain masters > regular g2g xfer? Does is colonize faster on agar? How is it cleaner....people say it's clean xfer every time...but cant airborn bacterias get on your agar soon as your about to xfer? then get into your fresh grains....the wedge might be resistant to contam, but the fresh sterilized grain isn't.
Other way around actually. Most agar formulations used support a wide variety of growth. Contaminants are easily visible on it as compared to other substrates. Molds like Trich are near impossible to identify in a jar before they start sporulating but the difference is apparent on agar.
But like I said, I think your main problem is with your g2g method which involves actually touching the substrate. Agar is certainly a good way to eliminate virtually all contamination caused by contaminated spores.
I avoid g2gs because the amount of spawn I produce is limited solely by the amount of substrate jars I'm willing to prepare, or in other words if I prepare 32 jars a month I'm going to wind up with 32 jars of spawn regardless of whether I g2g them or inoculate them with agar. However, for someone looking to inoculate as many jars as possible during in a period of time g2gs are the best way to do that.
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Oeric McKenna
LIFE CAPS


Registered: 06/15/12
Posts: 5,318
Loc: Babylon
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Re: f**kin trich, every time!!! [Re: Kizzle]
#18419410 - 06/14/13 03:43 PM (11 years, 7 months ago) |
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I really recommend the "in jar" pasteurization methods. You can run a drier substrate so the mycelium can race through it fast. Plus it minimizes post pasteurization handling. Lastly, you can get the temps held perfectly this way. Sounds like you went too high on the temps. That's a big problem usually.
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cronicr



Registered: 08/07/11
Posts: 62,904
Loc: Van Isle
Last seen: 2 minutes, 50 seconds
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Quote:
ConcreteWaves said:
Quote:
cronicr said: and when you do wedges u use a sab gb or fh
sab gb or fh? >.> 
still air box-glovebox-flowhood!
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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Satanschild
goodbye

Registered: 01/16/13 
Posts: 281
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Re: f**kin trich, every time!!! [Re: cronicr]
#18422784 - 06/15/13 10:47 AM (11 years, 7 months ago) |
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Still Air Box should be fine: http://www.shroomery.org/forums/showflat.php/Number/4359637 You'll find plenty more on the forum.
You can get agar powder from ebay or asian stores. I use glass petri dishes because I always PC them, plastic might melt but there are also autoclavable plastic petris. Simple recipe is 200 ml water, 5 gr agar, 5 gr of plain sugar(normally not recommended, but works just fine), and 40 gr of patato for about 15-20 petri dishes.
"Someone told me, if u wanna clone a rlly nice shroom u got, cut the stem in half, and take a tiny little centimeter chunk from the middle of the stem, and put it on agar." To be more exact, you have to tear the stem in half so you can expose unexposed tissue which you cut out after having steralized your scalpel(flamed until red hot) and quickly put on the agar.
And clean your tubs visciously after being contaminated by spores.
Edited by Satanschild (06/15/13 10:54 AM)
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tropicalfrenzy
Strangerer


Registered: 09/04/12
Posts: 1,522
Loc: Oz
Last seen: 10 years, 10 months
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Re: f**kin trich, every time!!! [Re: Satanschild]
#18432117 - 06/17/13 01:57 PM (11 years, 6 months ago) |
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pasteurizing too hot 170+ (semi/sterilizing in fact). So the second your sub comes out it contaminates in the air.
140-160 for an hour after core temp hits the sweet spot. Bring down the stove heat when core hits 150, it will keep going up.
I fill my pc with water 4/5 up the jars, Fill jars and cover with foil. bring to boil and turn down to simmer, wait for right temp. Do the monkey dance turning on and off the heat till the temp stabilizes (i know the sweet spot on my stove now) then wait an hour... really resilient to contam after this. A plate on top of the jars stops them floating away...
I've never used coir, only horse dung. But the process is the same I think.
Kizzles suggestion is the money. Leave some bulk unspawned and see if it contams, then you know you can work on your pasteurization process...
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tropicalfrenzy
Strangerer


Registered: 09/04/12
Posts: 1,522
Loc: Oz
Last seen: 10 years, 10 months
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errr, ummm... I was responding to the first page
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Edited by tropicalfrenzy (06/17/13 02:02 PM)
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plut0
Stranger

Registered: 01/03/19
Posts: 35
Last seen: 3 years, 6 months
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Re: f**kin trich, every time!!! *DELETED* [Re: ConcreteWaves]
#26829306 - 07/17/20 03:54 PM (4 years, 5 months ago) |
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Post deleted by plut0
Reason for deletion: lol
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chromeleon
Stranger


Registered: 07/05/20
Posts: 46
Last seen: 4 years, 2 months
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Re: f**kin trich, every time!!! [Re: plut0]
#26829409 - 07/17/20 04:45 PM (4 years, 5 months ago) |
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This post is 7 years old, hopefully he's figured it out by now.
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