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OfflineMycobro420
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Re: Clean spawn [Re: Roger Clemency]
    #26833243 - 07/19/20 09:04 PM (3 years, 6 months ago)

I think I'm doing to many qt jars and my an alot of agar to put in each one so my cleanliness slips doing so much. I'm going to upgrade my pc as well.iv had mixed success with bags and agar.this is first time doing so many qts with agar. most look good too soon to tell I'll update wen more jars and bins are fully colinized


Edited by Mycobro420 (07/19/20 09:05 PM)


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OfflineMycobro420
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Re: Clean spawn [Re: Mycobro420]
    #26833251 - 07/19/20 09:11 PM (3 years, 6 months ago)

Why I was thinking about lc is I would pick my best plate and cut wedge an place it in lc to insure it's clean in front of flow hood. Then use it to inoculate bags I figure less contam risk because I only have to grow the lc out from little peice of myc instead of having to do alot of dishes with alot more transfer peices and diff agar drops.and instead of using lc syringe inoculation dump it into each bag


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InvisibleRoger Clemency
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Re: Clean spawn [Re: Mycobro420]
    #26833771 - 07/20/20 07:48 AM (3 years, 6 months ago)

I personally use LI for all my jars now. I love it. I do have an LC ready to go as well but with LC you have to worry about keeping it clean after you inoculate your spawn, until you’re down to the last of the LC.

With LI you just need a clean plate and a jar of sterilized water and you make it up right before inoculation, use it up and that’s that.


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OfflineMycobro420
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Re: Clean spawn [Re: Roger Clemency]
    #26833826 - 07/20/20 08:40 AM (3 years, 6 months ago)

I used LL like I said earlier for one bag with 4qts in it fully colinized in two weeks I shook it about 7 days in. It colinized bin in 9 days and is fruiting now.but I'm convinced that I'm doin to much at once being so new to agar. IV done 100s of qts of rye with spore syring with almost no failer.then did small run with agar went well now I'm trying to do alot more lolol.so first bigger grow with agar I did around 20 bags all went well colinzation wise.some bags had dry grain on top each bag got 5 hydrated qts an I g2g with whole qt wen I put them to monotubs around half tricked after or before first flush but I figured the trick came from understerlized grain cause I only own a 23qt preso PC and tryed to do 40qts of grain in one pc with no spacers. It was totally filled.still got good yeild from ones that made it.so this time I did alot more agar an qts cause I figured my contamination was from understerlized grain cause my agar looks ok check then new pic I uploaded it has rizomorphic grow on first dish.so I did this cycle with half qts and bags with only 4qts each bags did alot better and qts are doing ok to. But to soon to tell.


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OfflineMycobro420
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Re: Clean spawn [Re: Mycobro420]
    #26833833 - 07/20/20 08:47 AM (3 years, 6 months ago)

And with the lc my idea is to use it all at once.so with LL is same amount of precaution as lc in my eyes.but with LL you know exactly the amount of myc going into water.with lc you re grow the myc out so in comparison 150mi of grown out lc would have much more active growing myc.so it's like having 5 dishes in little jar an I would dump the jar from bag to bag.so instead of having agar dish cut 4 ways to 4qts of grain that's 4 diff chances for something to go wrong wen I figure if have one grown out jar I can dump into 20 bags so I know all 20 bags came from the leading edge of a Petri dish.instad of using a bunch of wedges and g2g.it would also save time instead of waiting for qt jars to fully colinized to even be able to do g2gs


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