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Gan
Wielder of Narya



Registered: 08/26/19
Posts: 927
Loc: Valinor
Last seen: 5 months, 27 days
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Re: Sterile lab checklist [Re: AtmozFear]
#26830516 - 07/18/20 10:24 AM (3 years, 6 months ago) |
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They have water baths that maintain a good temp for agar to always be ready to pour. But a lab grade one can be pretty expensive. A decent, cheaper alternative is a sous vide
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AtmozFear
just a shade of myself


Registered: 01/25/19
Posts: 1,032
Last seen: 1 day, 15 hours
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Re: Sterile lab checklist [Re: Gan]
#26830519 - 07/18/20 10:26 AM (3 years, 6 months ago) |
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well I want one 
is there any down side to the sous vide? I just looked it up on Amazon. in my price range.
Edited by AtmozFear (07/18/20 10:28 AM)
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Re: Sterile lab checklist [Re: bodhisatta]
#26830523 - 07/18/20 10:28 AM (3 years, 6 months ago) |
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Quote:
bodhisatta said: Lol. But realistic. My lab for 80% of my mushroom growing was a living room table and plastic tub. Some gloves and rubbing alcohol.
You know people who need to buy the same running shoes Olympic athletes use before trying running out. Vs I go for a run in jeans and work shoes people.
I mean I agree but op says they have a 500sqft area with 2 offices. Shit if I had money to burn and the stuff op already lists as having I'd go all out 
500sqft space with nothing but a table and SAB seems like a funny image but a waste of space
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maxmush
Always learning...
Registered: 06/13/20
Posts: 440
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Re: Sterile lab checklist [Re: AtmozFear]
#26830527 - 07/18/20 10:29 AM (3 years, 6 months ago) |
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Sous vide machine is awesome for water bath.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Sterile lab checklist [Re: AtmozFear]
#26830528 - 07/18/20 10:29 AM (3 years, 6 months ago) |
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Expensive way to poach eggs i guess.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Quote:
natedawgnow said:
Quote:
bodhisatta said: Lol. But realistic. My lab for 80% of my mushroom growing was a living room table and plastic tub. Some gloves and rubbing alcohol.
You know people who need to buy the same running shoes Olympic athletes use before trying running out. Vs I go for a run in jeans and work shoes people.
I mean I agree but op says they have a 500sqft area with 2 offices. Shit if I had money to burn and the stuff op already lists as having I'd go all out 
500sqft space with nothing but a table and SAB seems like a funny image but a waste of space
More room for colonizing and fruiting XD
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AtmozFear
just a shade of myself


Registered: 01/25/19
Posts: 1,032
Last seen: 1 day, 15 hours
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Re: Sterile lab checklist [Re: bodhisatta]
#26830539 - 07/18/20 10:35 AM (3 years, 6 months ago) |
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Bod you are a true American. I mean that as a compliment.
I remember a documentary about tracking down Al Qaeda with this southern guy who seemed like a simple man but could speak Arabic, Farsi, Urdu...
Edited by AtmozFear (07/18/20 10:36 AM)
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One of Us
Stranger



Registered: 03/12/12
Posts: 1,140
Last seen: 6 days, 16 hours
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Re: Sterile lab checklist [Re: AtmozFear]
#26830541 - 07/18/20 10:37 AM (3 years, 6 months ago) |
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The water bath I have is for use in labs. They can be pretty expensive tho, but I got mine at an amazing price. I lucked out and found one for auction on ebay starting at $50. I was the only bidder 
Here's the model I have: Fisher Scientific Isotemp 205 y'all can fight over it
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maxmush
Always learning...
Registered: 06/13/20
Posts: 440
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Re: Sterile lab checklist [Re: maxmush]
#26830546 - 07/18/20 10:39 AM (3 years, 6 months ago) |
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Listen i get it, you can do everything at home in a SAB and sterile tek.
My particular situation is a little different. I have investors looking into the medicinal and gourmet market which I will be assisting them with. They will be funding the lab for my work. I have 500sqft office to build. I need to get a shopping list together by Monday
Thanks everyone for participating in this thread, it will be really helpful.
On my wishlist so far:
1. large capacity steam autoclave 150l-360l 2. desktop autoclave 24l-80l 3. 2 person laminar flow hood and cabinet with horizontal flow, UV sterilization light, H14 HEPA 4. water bath/sous vide 5. fridge with window 6. stainless tables on castors 7. wire shelving 8. magnetic stirrers x 4 9. Maybe a bead sterilizer for tools 10. comfy chair
Misc lab supplies:
1. petri dishes 2. media bottles 3. maybe a microscope
What else??
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AtmozFear
just a shade of myself


Registered: 01/25/19
Posts: 1,032
Last seen: 1 day, 15 hours
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Re: Sterile lab checklist [Re: One of Us]
#26830548 - 07/18/20 10:40 AM (3 years, 6 months ago) |
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does the agar stay sterile?
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maxmush
Always learning...
Registered: 06/13/20
Posts: 440
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Re: Sterile lab checklist [Re: bodhisatta]
#26830549 - 07/18/20 10:41 AM (3 years, 6 months ago) |
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Quote:
bodhisatta said: Expensive way to poach eggs i guess.
It'll turn a shitty steak into fillet quality. Even cooking temp throughout and will never over cook. The best thing i ever got to cook. Its a total game changer.
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AtmozFear
just a shade of myself


Registered: 01/25/19
Posts: 1,032
Last seen: 1 day, 15 hours
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Re: Sterile lab checklist [Re: maxmush]
#26830551 - 07/18/20 10:43 AM (3 years, 6 months ago) |
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like natedawg said, this shit is so fun to have... I love new toys
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Re: Sterile lab checklist [Re: maxmush]
#26830552 - 07/18/20 10:45 AM (3 years, 6 months ago) |
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I'm tellin ya a cement mixer is seriously awesome for mixing up sawdust substrate.
After a few times mixing by hand you'll regret not having one.
What are you planning on using as sub actually? I guess if you're using straw or something you won't need a mixer.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Cutting straw sucks ass with scissors. 6 gal bucket and a lid with a hole that fits a weed whacker tho.
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maxmush
Always learning...
Registered: 06/13/20
Posts: 440
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Quote:
natedawgnow said: I'm tellin ya a cement mixer is seriously awesome for mixing up sawdust substrate.
After a few times mixing by hand you'll regret not having one.
What are you planning on using as sub actually? I guess if you're using straw or something you won't need a mixer.
We have been using a cement mixer attachment to a drill but have been looking into the standing drum mixer you are referring to as I have shredded 2 of them already lol. Using the typical CVG 1:1.
We are looking into contracting a local supply store to pre-pasturize our subs. i just dont know how long they will stay pasteurized however. (i also question if its even necessary as I have done a bunch with zero pasteurization with the same results)
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Re: Sterile lab checklist [Re: maxmush]
#26830573 - 07/18/20 11:00 AM (3 years, 6 months ago) |
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Do it. Look on craigs and shit and you may find one for relatively cheap.
@bod-I've never used straw for anything. I do all my edibles on supp sawdust blocks, but if I did I'd do that for sure. Unless I was pumping out quantity, in which case I'd get a shredder 
Straw just seems like such a pain in the ass. I've heard oysters perform better on straw but I dont run oysters much.
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Gan
Wielder of Narya



Registered: 08/26/19
Posts: 927
Loc: Valinor
Last seen: 5 months, 27 days
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Re: Sterile lab checklist [Re: maxmush]
#26830578 - 07/18/20 11:03 AM (3 years, 6 months ago) |
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Quote:
maxmush said:
On my wishlist so far:
1. large capacity steam autoclave 150l-360l 2. desktop autoclave 24l-80l 3. 2 person laminar flow hood and cabinet with horizontal flow, UV sterilization light, H14 HEPA 4. water bath/sous vide 5. fridge with window 6. stainless tables on castors 7. wire shelving 8. magnetic stirrers x 4 9. Maybe a bead sterilizer for tools 10. comfy chair
Misc lab supplies:
1. petri dishes 2. media bottles 3. maybe a microscope
Just a heads up, all that listed is more than likely going to be way out of budget unless you go the used route. A steam autoclave that large and the desktop autoclave will pretty much take up the entire budget on their own. An AA75x (for the desktop autoclave) for example is roughly 39 liters and sits over $1000 after taxes and shipping. Just something to keep in mind as you budget.
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El Chupacabra
Stoned Ape



Registered: 01/07/19
Posts: 676
Loc: 🇺🇸
Last seen: 1 year, 16 days
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Re: Sterile lab checklist [Re: maxmush]
#26830584 - 07/18/20 11:10 AM (3 years, 6 months ago) |
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Bod is a minimalist. I can appreciate a minimalist HOWEVER, I think there's an argument to be made for somebody who spends damn good money in this hobby to have what they feel are the appropriate tools and environment I know speaking from my own experience it forced me to not give up no matter my shortcomings and failures because it would be like burning a pile of cash. On the other hand I can see how a person could fuck themselves a little (or a lot) in the wallet. Seems like this could easily go in the financial advice forum or whatever it's called here on shroomary, if we're talking purely cultivation advice then fucking do it OP.
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maxmush
Always learning...
Registered: 06/13/20
Posts: 440
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Re: Sterile lab checklist [Re: Gan]
#26830586 - 07/18/20 11:11 AM (3 years, 6 months ago) |
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Thanks Gan. I have found several suppliers on Alibaba for the autoclaves and flow hood station that are reasonable and will be about $7k.
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Martinsapin
no-mad



Registered: 09/12/16
Posts: 310
Loc: Canada
Last seen: 1 year, 7 months
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Re: Sterile lab checklist [Re: maxmush]
#26830696 - 07/18/20 12:30 PM (3 years, 6 months ago) |
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OP do you even grow bro?
-------------------- looking for a sclerotia producer print
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