Home | Community | Message Board

MagicBag Grow Bags
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Bulk Cannabis Seeds   Kraken Kratom Red Vein Kratom   Mushroom-Hut Mono Tub Substrate   Bridgetown Botanicals CBD Concentrates   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Kratom Powder for Sale   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   MagicBag.co All-In-One Bags That Don't Suck   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Myyco.com Golden Teacher Liquid Culture For Sale

Jump to first unread post Pages: < First | < Back | 284 | 285 | 286 | 287 | 288 | 289 | 290 | 291 | 292 | 293 | 294 | 295 | 296 | 297 | 298 | 299 | 300 | 301 | 302 | 303 | 304 | Next > | Last >
OfflineCelestialexplorer1
Male


Registered: 03/25/20
Posts: 1,803
Loc: Floating in a tin can
Last seen: 4 months, 10 days
Re: The Official Woodlovers Thread [Re: Ferather]
    #26827236 - 07/16/20 03:39 PM (3 years, 8 months ago)

Thank you for that post ferather. I didn’t know about that, I’m pretty new to all this and there’s no telling how long it would have taken me to find this out since I’m in the process of starting some outdoor and indoor grows.

Can I ask what type of fertilizer you use. I’ve been doing research on fertilizers effects on mushrooms and actually just got some, micro-life


--------------------


To spend just one moment in eternity

Edited by Celestialexplorer1 (07/16/20 03:41 PM)

Extras: Filter Print Post Top
OfflineBobbit
Even Stranger


Registered: 05/30/18
Posts: 1,251
Loc: The outsideround Flag
Last seen: 11 days, 19 hours
Re: The Official Woodlovers Thread [Re: Ferather]
    #26828193 - 07/17/20 04:40 AM (3 years, 8 months ago)

Quote:

Ferather said:
There's not so much on the lemon tek and citric acid specifically, however try this (the reaction).

Link 1, Link 2, Link 3. Google Search: psilocybin dephosphorylation



Dude, there are quite a few newer papers on extraction, and it appears that most Psilocybe species produce phosphatases which is why the generally agreed method of preservation is to dry as quickly as possible. Any aqueous solution H2O, ethanol, acetic acid, etc. all pull the present phosphatases also, making them less stable due to dephosphorylation to psilocin, which is very easily oxidised. . .

This is the main reason I asked to to back up your claim, as if it were as easy as adding citric acid to both dephosphorylate psilocybin and make psilocin a more stable salt (psilocin citrate), would be ABSOLUTELY AMAZING. . .

It still seems that non-aqueous methanol extract to separate actives from phosphatases Is potentially the most likely pathway. . . Would love to be proved wrong. . .


--------------------
The Official Kiwi Cultivators Thread

Official New Zealand woodlovers grow thread

–––––    ╭∩╮(-_-)╭∩╮    –––––
The Official Kiwi DMT and MAOI Thread

Extras: Filter Print Post Top
OfflineAdas
Lonely Dreamer
Male User Gallery


Registered: 12/22/16
Posts: 5,288
Loc: Central EU
Last seen: 31 minutes, 10 seconds
Trusted Cultivator
Re: The Official Woodlovers Thread [Re: Bobbit]
    #26828221 - 07/17/20 05:26 AM (3 years, 8 months ago)

Keep in mind that certain solvents and pH ranges can render certain enzymes inactive (change their tertiary structure making them useless). So it's likely true for the phosphatase as well, we just don't know what exactly, but IMO it's safe to say that low pH or concentrated alcohols inactivate it on contact.

Extras: Filter Print Post Top
OfflineBU4O
Weed on ^^
Male User Gallery


Registered: 11/18/11
Posts: 754
Loc: Bulgaria,Plovdiv Flag
Last seen: 7 hours, 6 minutes
Re: The Official Woodlovers Thread [Re: Adas]
    #26828238 - 07/17/20 05:43 AM (3 years, 8 months ago)

So my vodka with all the dried libs and allenii from 2019 is useless, I am so so unhappy right now :cryariver:  :crymeariver:  :bestcry:


--------------------

Extras: Filter Print Post Top
OfflineAdas
Lonely Dreamer
Male User Gallery


Registered: 12/22/16
Posts: 5,288
Loc: Central EU
Last seen: 31 minutes, 10 seconds
Trusted Cultivator
Re: The Official Woodlovers Thread [Re: BU4O]
    #26828239 - 07/17/20 05:44 AM (3 years, 8 months ago)

Ehm... Quite the opposite actually :rofl:

But you should try anyway, I could be wrong.

Extras: Filter Print Post Top
OfflineBU4O
Weed on ^^
Male User Gallery


Registered: 11/18/11
Posts: 754
Loc: Bulgaria,Plovdiv Flag
Last seen: 7 hours, 6 minutes
Re: The Official Woodlovers Thread [Re: Adas]
    #26828326 - 07/17/20 07:09 AM (3 years, 8 months ago)

I did leave it in cold dry place away from light so it can extract more...
I have plenty of dry mushrooms various kinds so I did want to store them that way...


--------------------

Extras: Filter Print Post Top
OfflineFerather
Mycological
Male User Gallery


Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom Flag
Last seen: 1 year, 4 months
Re: The Official Woodlovers Thread [Re: BU4O]
    #26828459 - 07/17/20 08:53 AM (3 years, 8 months ago)

@Bobbit, no you are correct, I did say in my original post about the normal-better method, with alcohol.
@Celestialexplorer1, checkout my "Growing Mushrooms" post in my journal.

https://www.youtube.com/watch?t=2256&v=y7mdAS7KlRw


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.

Edited by Ferather (07/17/20 09:05 AM)

Extras: Filter Print Post Top
Offlineanon1978
Stranger
Male

Registered: 07/17/20
Posts: 3
Last seen: 1 year, 8 months
Re: The Official Woodlovers Thread [Re: Ferather]
    #26830064 - 07/18/20 01:24 AM (3 years, 8 months ago)

@Ferather What do you mean when you say ‘inhibitory materials’?

Extras: Filter Print Post Top
OfflineBobbit
Even Stranger


Registered: 05/30/18
Posts: 1,251
Loc: The outsideround Flag
Last seen: 11 days, 19 hours
Re: The Official Woodlovers Thread [Re: Adas]
    #26830237 - 07/18/20 06:16 AM (3 years, 8 months ago)

Quote:

BU4O said:
So my vodka with all the dried libs and allenii from 2019 is useless, I am so so unhappy right now :cryariver:  :crymeariver:  :bestcry:



Remains to be seen. . . Testing is in order. . .

Quote:

Adas said:
Keep in mind that certain solvents and pH ranges can render certain enzymes inactive (change their tertiary structure making them useless). So it's likely true for the phosphatase as well, we just don't know what exactly, but IMO it's safe to say that low pH or concentrated alcohols inactivate it on contact.



Many phosphatases are alkaline so lowering pH isn’t necessarily a positive. . . Phosphatases seem to often have denaturation temps of 85+°C also, and become more active at temps above room temp and heat denaturing will kill psilicybin before the phosphatases. . .


That is why I was hassling Ferather for real info on psilocin citrate (or any salt that is stable) so the phosphatases are not an issue as psilocyn has no phosphate group. As long as there is a stable salt, it may be extractable. . .

Some of the early research suggests that acidic (acetic) extracts seem to accelerate dephosphorylation, resulting in no actives. . .

I wish I had paid more attention in Bio and Chem. . .

And should prolly end up back on topic. . .


--------------------
The Official Kiwi Cultivators Thread

Official New Zealand woodlovers grow thread

–––––    ╭∩╮(-_-)╭∩╮    –––––
The Official Kiwi DMT and MAOI Thread

Extras: Filter Print Post Top
OfflineFerather
Mycological
Male User Gallery


Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom Flag
Last seen: 1 year, 4 months
Re: The Official Woodlovers Thread [Re: Bobbit] * 1
    #26830299 - 07/18/20 07:39 AM (3 years, 8 months ago)

@Bobbit, your body will dephosphorylate psilocybin into psilocyn, at this point is can pass into the bloodstream and brain barrier.
The lemon tek, makes the psilocybin more susceptible to dephosphorylation (like stomach acid), speeds it up.

It can only speed up the conversion process, not increase potency (total amount).

----

Extraction Procedures: http://www.pbso.org/qualtrax/QTDocuments/1968.PDF

----

@anon1978, https://www.sciencedirect.com/science/article/abs/pii/S0964830512002843
Various materials-chemicals in wood are anti-fungal and can inhibit growth.

Good examples are fresh pine wood, or cedar wood.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.

Extras: Filter Print Post Top
OfflineBobbit
Even Stranger


Registered: 05/30/18
Posts: 1,251
Loc: The outsideround Flag
Last seen: 11 days, 19 hours
Re: The Official Woodlovers Thread [Re: Ferather]
    #26831051 - 07/18/20 03:49 PM (3 years, 8 months ago)

Your links speculate where in the body this happens, yes. . . Not looking to ‘strengthen’. . .

Internal dephosphorylation doesn’t really help with extractions though :shrug:

It would be really nice to be able to have a stable solution containing actives for microdosing. . .

This is why I’m keen to keep asking the questions though there are many looking and no-one really finding consistent results. . .

As cool as it would be to have a dose in a few drops, just being stable in solution is enough. . .

For now it seems that anhydrous methanol will pull all the SillySimon, and ignore the phosphatases. . . The only consistent ‘easy’ tek for the kitchen chemist. . . I’m thinking trying this then evaping all the methanol, then adding ethanol for tincture. . .

Maybe adding a peptidase to eat the enzymes?

This convo appears periodically, yet peters out before anything concrete lands. . .


--------------------
The Official Kiwi Cultivators Thread

Official New Zealand woodlovers grow thread

–––––    ╭∩╮(-_-)╭∩╮    –––––
The Official Kiwi DMT and MAOI Thread

Extras: Filter Print Post Top
OfflineAdas
Lonely Dreamer
Male User Gallery


Registered: 12/22/16
Posts: 5,288
Loc: Central EU
Last seen: 31 minutes, 10 seconds
Trusted Cultivator
Re: The Official Woodlovers Thread [Re: Bobbit]
    #26831078 - 07/18/20 04:04 PM (3 years, 8 months ago)

The only ways to avoid oxidation is:

1. Restrict access to Oxygen
2. Put enough antioxidant into the solution (Vitamin C)

Extras: Filter Print Post Top
OfflineBobbit
Even Stranger


Registered: 05/30/18
Posts: 1,251
Loc: The outsideround Flag
Last seen: 11 days, 19 hours
Re: The Official Woodlovers Thread [Re: Adas]
    #26831308 - 07/18/20 06:22 PM (3 years, 8 months ago)

3. Create a stable salt that doesn't oxidise readily . . .

If such a salt exists. . .


--------------------
The Official Kiwi Cultivators Thread

Official New Zealand woodlovers grow thread

–––––    ╭∩╮(-_-)╭∩╮    –––––
The Official Kiwi DMT and MAOI Thread

Extras: Filter Print Post Top
OfflineAdas
Lonely Dreamer
Male User Gallery


Registered: 12/22/16
Posts: 5,288
Loc: Central EU
Last seen: 31 minutes, 10 seconds
Trusted Cultivator
Re: The Official Woodlovers Thread [Re: Bobbit]
    #26831917 - 07/19/20 04:23 AM (3 years, 8 months ago)

I don't think the salt form matters when it comes to oxidation. I highly doubt that such a salt exists (unless the acid has some reducing power, like ascorbic acid).

Extras: Filter Print Post Top
OfflineFerather
Mycological
Male User Gallery


Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom Flag
Last seen: 1 year, 4 months
Re: The Official Woodlovers Thread [Re: Adas] * 1
    #26832649 - 07/19/20 02:40 PM (3 years, 8 months ago)

@Bobbit, The lemon tek is not an extraction method, and certainly would not be employed to do so.
It's meant to speed up conversion, prior to use (its pretty much used instantly).

Like adding conversion accelerant to water then mushrooms.

---

Digging deep, psilocin-citrate is probs false.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.

Extras: Filter Print Post Top
OfflineCelestialexplorer1
Male


Registered: 03/25/20
Posts: 1,803
Loc: Floating in a tin can
Last seen: 4 months, 10 days
Re: The Official Woodlovers Thread [Re: Ferather] * 1
    #26832825 - 07/19/20 04:18 PM (3 years, 8 months ago)


White spots of myc turning blue after I tried to emulate a cold snap. After that it started to come through a lot more. Still no primordia in sight.


--------------------


To spend just one moment in eternity

Extras: Filter Print Post Top
OfflineBobbit
Even Stranger


Registered: 05/30/18
Posts: 1,251
Loc: The outsideround Flag
Last seen: 11 days, 19 hours
Re: The Official Woodlovers Thread [Re: Adas]
    #26833231 - 07/19/20 08:48 PM (3 years, 8 months ago)

Quote:

Ferather said:
@Bobbit, The lemon tek is not an extraction method, and certainly would not be employed to do so.
It's meant to speed up conversion, prior to use (its pretty much used instantly).

Like adding conversion accelerant to water then mushrooms.

---

Digging deep, psilocin-citrate is probs false.



I understand why peeps use lemon. . . Because :awesome:. . .
If there was a stable salt, that would be one step in the process of extraction, though I suspect that the isn't with any of the obvious acid/bases used for extracts. . .

Quote:

Adas said:
I don't think the salt form matters when it comes to oxidation. I highly doubt that such a salt exists (unless the acid has some reducing power, like ascorbic acid).



I thought different salts were more stable than others. . . Some being more susceptible than others to oxidation?
NaCl for example is pretty stable in air. . . Hydrophilic yet stable in the presence of O2. . .

I should have paid more attention in chemistry for sure. . .


--------------------
The Official Kiwi Cultivators Thread

Official New Zealand woodlovers grow thread

–––––    ╭∩╮(-_-)╭∩╮    –––––
The Official Kiwi DMT and MAOI Thread

Extras: Filter Print Post Top
OfflineAdas
Lonely Dreamer
Male User Gallery


Registered: 12/22/16
Posts: 5,288
Loc: Central EU
Last seen: 31 minutes, 10 seconds
Trusted Cultivator
Re: The Official Woodlovers Thread [Re: Bobbit]
    #26833537 - 07/20/20 02:53 AM (3 years, 8 months ago)

NaCl is a highly stable inorganic salt, lol. Has nothing to do with organic salts. In salts of alkaloids, the alkaloid cation is like the sodium and the acid anion is like the chloride. Doesn't really matter what anion is the alkaloid surrounded by, if it's susceptible to oxidation, it always will be.

Acids can stabilize things like hydrogen peroxide but that's a different thing.

Extras: Filter Print Post Top
OfflineCelestialexplorer1
Male


Registered: 03/25/20
Posts: 1,803
Loc: Floating in a tin can
Last seen: 4 months, 10 days
Re: The Official Woodlovers Thread [Re: Adas]
    #26833785 - 07/20/20 07:57 AM (3 years, 8 months ago)

So I think like most people who when they get started with woodlovers and exotics think or about or attempt cult indoors. I’ve seen some decent grows indoors as well. A lot say it’s very difficult, time
Consuming and next to impossible. But I’ve had this idea in my head ever since I saw the refrigerator tek awhile back. I’ve seen tons of DIY mini drives as well. So put two and two together.

My idea which is already under way is

1 semiconductor thermoelectric refrigeration unit.
1 power supply with cord
1 thermo control panel

I have all this and it wasn’t expensive $70 or someone could harvest these from an old mini fridge
So I make a box out of plexiglass and insulation with a compartment for the hardware. I also make a plexiglass lid with latch and weather strip to seal it. I put a light a foot or so above 6500k. I make a chamber on the bottom that holds water and put two of those small disc fogger a in it since cold air doesn’t hold humidity but in 45-60 f the fogger s should do fine at filling up the whole unit. The problem I see is air exchange. I’m making this thing right now wether you like it or not lol. This post will probably get brushed off as pipe smoke but I’m in it for the long haul. Just curious if there is anyone out there with skills relating that might shed some insight into how I could get some proper air exchange without having to scrap the semiconductor and do an evaporative fan instead. Anybody
Love to the people.


--------------------


To spend just one moment in eternity

Extras: Filter Print Post Top
OfflineFerather
Mycological
Male User Gallery


Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom Flag
Last seen: 1 year, 4 months
Re: The Official Woodlovers Thread [Re: Celestialexplorer1]
    #26833796 - 07/20/20 08:16 AM (3 years, 8 months ago)

@Celestialexplorer1, any setup intended to grow King oyster indoors with regulated temperatures will work, it will be triggers with other species.
Stick with the plant pot method, you can simulate heavy rain simply by overwatering it, and let the excess drain.

Fruiting requirements (nutrients-temp-humidity-oxygen) > triggers.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.

Extras: Filter Print Post Top
Jump to top Pages: < First | < Back | 284 | 285 | 286 | 287 | 288 | 289 | 290 | 291 | 292 | 293 | 294 | 295 | 296 | 297 | 298 | 299 | 300 | 301 | 302 | 303 | 304 | Next > | Last >

Shop: Original Sensible Seeds Bulk Cannabis Seeds   Kraken Kratom Red Vein Kratom   Mushroom-Hut Mono Tub Substrate   Bridgetown Botanicals CBD Concentrates   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Kratom Powder for Sale   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   MagicBag.co All-In-One Bags That Don't Suck   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Myyco.com Golden Teacher Liquid Culture For Sale


Similar ThreadsPosterViewsRepliesLast post
* The Official Semilanceata Thread
( 1 2 3 4 ... 22 23 )
Baba Yaga 20,201 443 03/28/24 01:17 AM
by Blueberry Muffin
* Sporeless Cubensis? Herbus 4,522 16 02/11/05 01:57 PM
by scatmanrav
* cloning, sectors, isolates? thisone 3,311 10 11/07/03 10:53 PM
by MycoCat
* Multi Spore Grain to Grain & Senescence Crabmeat 5,518 3 07/12/08 08:51 PM
by Bubba McMushies
* Sporeless Fruit Magash 2,031 9 09/06/02 08:55 PM
by Zen Peddler
* Sterile mushrooms (sporeless) <pic> dirk gently 2,009 8 06/21/02 10:23 AM
by World Spirit
* sporeless gills? zazz 1,086 5 10/13/02 11:08 PM
by zazz
* My first sporeless mutants... mycocurious 824 2 11/10/07 06:26 PM
by mycocurious

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
479,397 topic views. 23 members, 133 guests and 97 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.047 seconds spending 0.018 seconds on 16 queries.