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mysticalshroom975
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Registered: 07/03/20
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Nonviable spore syringes? Agar issues.
#26828099 - 07/17/20 02:01 AM (3 years, 6 months ago) |
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I got syringes from two different vendors on reddit.com/r/sporetraders and put them on agar with practically zero results. One type of agar I used was my own recipe of 2% potato starch, 2.5% maple syrup, 2% agar. Later I used the standard malt recipe. I was only able to get one to colonize, shown below. They were transferred to agar by filtering a sterile q-tip then swabbing the agar. I wrote another post on this as I suspect it may be contamination. See the post for more details
It may have stayed in the mail box for a little while, I had it shipped to a friends house. I don't think that it got that hot, we're in Vancouver WA. It gets hot in the summer, but I don't recall it being too hot. Do you think these could be poor quality spore samples and I should ask for a refund? Or perhaps they're cooked in transport?
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mushpunx
Fungus Punk



Registered: 04/20/14
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No need to start a second thread man
How fast is it growing? It looks suspect to me, like mold.
I would give the syringes another try, spores can be stubborn to germinate sometimes.
--------------------
 Amateur Mycologists United AMU Q&A
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mysticalshroom975
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Re: Nonviable spore syringes? Agar issues. [Re: mushpunx]
#26828119 - 07/17/20 02:30 AM (3 years, 6 months ago) |
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I think there's about four days of growth in that picture. Seven days ago, I did a second inoculation on malt agar of all three syringes using the q-tip method I mentioned before and they show zero growth.
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redhandmat
Dude


Registered: 05/09/19
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Quote:
mysticalshroom975 said: I think there's about four days of growth in that picture. Seven days ago, I did a second inoculation on malt agar of all three syringes using the q-tip method I mentioned before and they show zero growth.
Dude ime some prints/syringes give full plates by day 10 while others take like 2 weeks to show initial growth. definitely do more plates. Are you working with prints or syringes?
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mysticalshroom975
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Re: Nonviable spore syringes? Agar issues. [Re: redhandmat]
#26828172 - 07/17/20 04:06 AM (3 years, 6 months ago) |
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Quote:
redhandmat said:
Quote:
mysticalshroom975 said: I think there's about four days of growth in that picture. Seven days ago, I did a second inoculation on malt agar of all three syringes using the q-tip method I mentioned before and they show zero growth.
Dude ime some prints/syringes give full plates by day 10 while others take like 2 weeks to show initial growth. definitely do more plates. Are you working with prints or syringes?
Working with syringes. Also, I thought that by concentrating the spores in a q-tip and having the temp at 80F that would provide good conditions for quick germination. You've had to wait for ~2weeks for mycelium with warm conditions like 80F?
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roarkell
The Roar Master!



Registered: 08/29/19
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I've had some take 10+ days to germ... another thing I used to do when using swabs from syringe was I'd let the syringe sit in the sab, needle on, pointed down (usually just set it in a pint jar) overnight... then do your drops to swab(s)... whatever solids (hopefully spores) are in your solutions should sink down towards the needle.
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redhandmat
Dude


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I just work in room temperature, I don't own a thermometer so I dont know exact temperature. And Ive done agar work during winter months and summer season, inside my home and it has worked fine everytime. I dont use qtips. I just use an inoculation loop, flamed, cooled and then take spores from print and put them on the plate. You have syringe so I would just shake it well and squirt a tiny drop right in the middle of the plate and let it set.
Dont think too much about quick germination, it will happen when it happens. Just think of working as clean as you can using proper techniques. Keep it simple, don't use heat mats or what have you. Cube myc grows just fine in room temperatures where a human can live comfortably. If its on the colder side then it goes a little slower, if it is on the hotter side you may get contams instead.
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mysticalshroom975
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Re: Nonviable spore syringes? Agar issues. [Re: roarkell]
#26828872 - 07/17/20 12:23 PM (3 years, 6 months ago) |
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Quote:
roarkell said: I've had some take 10+ days to germ... another thing I used to do when using swabs from syringe was I'd let the syringe sit in the sab, needle on, pointed down (usually just set it in a pint jar) overnight... then do your drops to swab(s)... whatever solids (hopefully spores) are in your solutions should sink down towards the needle.
Yeah, when I filtered the water throught the q-tip cotton, I filtered about 3-4 cc's through and there was some visible darkness on the cotton so I think I should have got a sufficient amount for germination and growth. It's just discouraging seeing zero growth after about nine days. I think the mycelium in the picture is contamination.
Edited by mysticalshroom975 (07/17/20 12:28 PM)
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roarkell
The Roar Master!



Registered: 08/29/19
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Whatever is going on in those pics doesnt look too promising... but that's why we use agar... at least your not shooting syringes straight to grain... lol. The only other thing I could suggest would be shoot me a PM and I can shoot you some prints... I've got plenty of variety if you wanna check my trade list. Working from a print is far easier IME.
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jcm4620
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Re: Nonviable spore syringes? Agar issues. [Re: roarkell]
#26829098 - 07/17/20 02:03 PM (3 years, 6 months ago) |
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well i got as far as the word redit then i stopped reading. but honestly man u should stay far fat away from that god awful place. those ppl there are complete hacks. that place is by far the worst place to get any kind of advice from. you are at the correct place now and i suggest you erase everything that you read or got told from there from your memory and start some fresh research here. and im sure someone will gladly send u a print. i know i would but all i have at the moment are pans
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Tormato  
The Goddess Kali Meh 😛




Registered: 07/01/17
Posts: 6,067
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Re: Nonviable spore syringes? Agar issues. [Re: jcm4620]
#26829113 - 07/17/20 02:16 PM (3 years, 6 months ago) |
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Quote:
jcm4620 said: well i got as far as the word redit then i stopped reading. but honestly man u should stay far fat away from that god awful place. those ppl there are complete hacks. that place is by far the worst place to get any kind of advice from. you are at the correct place now and i suggest you erase everything that you read or got told from there from your memory and start some fresh research here. and im sure someone will gladly send u a print. i know i would but all i have at the moment are pans
I second this....Reddit is a terrible source for Cultivation information.
-------------------- Helpful Threads The Shroomery Store Tormato's Q&A Thread Post Questions Here or PM me! "Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!
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roarkell
The Roar Master!



Registered: 08/29/19
Posts: 2,223
Loc: Southern WI
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Re: Nonviable spore syringes? Agar issues. [Re: Tormato]
#26829158 - 07/17/20 02:35 PM (3 years, 6 months ago) |
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Haha... I wasnt gonna knock reddit but when back to back TCs chime in, you know what's up... lol. Seriously like jcm said, check around the shroomery, theres a lot of super generous people here.
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redhandmat
Dude


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Re: Nonviable spore syringes? Agar issues. [Re: roarkell]
#26829184 - 07/17/20 02:50 PM (3 years, 6 months ago) |
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Quote:
roarkell said: Whatever is going on in those pics doesnt look too promising... but that's why we use agar... at least your not shooting syringes straight to grain... lol. The only other thing I could suggest would be shoot me a PM and I can shoot you some prints... I've got plenty of variety if you wanna check my trade list. Working from a print is far easier IME.
This guy right here is a savior, and I should know!
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