Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: PhytoExtractum Buy Bali Kratom Powder   Left Coast Kratom Buy Kratom Capsules   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures   Kraken Kratom Red Vein Kratom   North Spore Injection Grain Bag

Jump to first unread post Pages: 1
Offlinemysticalshroom975
Stranger


Registered: 07/03/20
Posts: 78
Last seen: 9 months, 2 days
Nonviable spore syringes? Agar issues.
    #26828099 - 07/17/20 02:01 AM (3 years, 6 months ago)

I got syringes from two different vendors on reddit.com/r/sporetraders and put them on agar with practically zero results.  One type of agar I used was my own recipe of 2% potato starch, 2.5% maple syrup, 2% agar.  Later I used the standard malt recipe.  I was only able to get one to colonize, shown below.  They were transferred to agar by filtering a sterile q-tip then swabbing the agar. I wrote another post on this as I suspect it may be contamination.  See the post for more details

It may have stayed in the mail box for a little while, I had it shipped to a friends house.  I don't think that it got that hot, we're in Vancouver WA.  It gets hot in the summer, but I don't recall it being too hot.  Do you think these could be poor quality spore samples and I should ask for a refund?  Or perhaps they're cooked in transport?




Extras: Filter Print Post Top
Offlinemushpunx
Fungus Punk
 User Gallery


Registered: 04/20/14
Posts: 13,394
Last seen: 10 days, 13 hours
Trusted Cultivator
Re: Nonviable spore syringes? Agar issues. [Re: mysticalshroom975] * 1
    #26828113 - 07/17/20 02:17 AM (3 years, 6 months ago)

No need to start a second thread man

How fast is it growing? It looks suspect to me, like mold.

I would give the syringes another try, spores can be stubborn to germinate sometimes.


--------------------

Amateur Mycologists United
AMU Q&A


Extras: Filter Print Post Top
Offlinemysticalshroom975
Stranger


Registered: 07/03/20
Posts: 78
Last seen: 9 months, 2 days
Re: Nonviable spore syringes? Agar issues. [Re: mushpunx]
    #26828119 - 07/17/20 02:30 AM (3 years, 6 months ago)

I think there's about four days of growth in that picture.  Seven days ago, I did a second inoculation on malt agar of all three syringes using the q-tip method I mentioned before and they show zero growth.


Extras: Filter Print Post Top
Offlineredhandmat
Dude


Registered: 05/09/19
Posts: 463
Last seen: 2 years, 8 months
Re: Nonviable spore syringes? Agar issues. [Re: mysticalshroom975]
    #26828126 - 07/17/20 02:51 AM (3 years, 6 months ago)

Quote:

mysticalshroom975 said:
I think there's about four days of growth in that picture.  Seven days ago, I did a second inoculation on malt agar of all three syringes using the q-tip method I mentioned before and they show zero growth.




Dude ime some prints/syringes give full plates by day 10 while others take like 2 weeks to show initial growth. definitely do more plates. Are you working with prints or syringes?


Extras: Filter Print Post Top
Offlinemysticalshroom975
Stranger


Registered: 07/03/20
Posts: 78
Last seen: 9 months, 2 days
Re: Nonviable spore syringes? Agar issues. [Re: redhandmat]
    #26828172 - 07/17/20 04:06 AM (3 years, 6 months ago)

Quote:

redhandmat said:
Quote:

mysticalshroom975 said:
I think there's about four days of growth in that picture.  Seven days ago, I did a second inoculation on malt agar of all three syringes using the q-tip method I mentioned before and they show zero growth.




Dude ime some prints/syringes give full plates by day 10 while others take like 2 weeks to show initial growth. definitely do more plates. Are you working with prints or syringes?



Working with syringes.  Also, I thought that by concentrating the spores in a q-tip and having the temp at 80F that would provide good conditions for quick germination.  You've had to wait for ~2weeks for mycelium with warm conditions like 80F?


Extras: Filter Print Post Top
Offlineroarkell
The Roar Master!
Male User Gallery


Registered: 08/29/19
Posts: 2,223
Loc: Southern WI Flag
Last seen: 6 months, 21 days
Re: Nonviable spore syringes? Agar issues. [Re: mysticalshroom975]
    #26828197 - 07/17/20 04:48 AM (3 years, 6 months ago)

I've had some take 10+ days to germ... another thing I used to do when using swabs from syringe was I'd let the syringe sit in the sab, needle on, pointed down (usually just set it in a pint jar) overnight... then do your drops to swab(s)... whatever solids (hopefully spores) are in your solutions should sink down towards the needle.


Extras: Filter Print Post Top
Offlineredhandmat
Dude


Registered: 05/09/19
Posts: 463
Last seen: 2 years, 8 months
Re: Nonviable spore syringes? Agar issues. [Re: mysticalshroom975]
    #26828201 - 07/17/20 04:51 AM (3 years, 6 months ago)

I just work in room temperature, I don't own a thermometer so I dont know exact temperature. And Ive done agar work during winter months and summer season, inside my home and it has worked fine everytime. I dont use qtips. I just use an inoculation loop, flamed, cooled and then take spores from print and put them on the plate. You have syringe so I would just shake it well and squirt a tiny drop right in the middle of the plate and let it set.

Dont think too much about quick germination, it will happen when it happens. Just think of working as clean as you can using proper techniques. Keep it simple, don't use heat mats or what have you. Cube myc grows just fine in room temperatures where a human can live comfortably. If its on the colder side then it goes a little slower, if it is on the hotter side you may get contams instead.


Extras: Filter Print Post Top
Offlinemysticalshroom975
Stranger


Registered: 07/03/20
Posts: 78
Last seen: 9 months, 2 days
Re: Nonviable spore syringes? Agar issues. [Re: roarkell]
    #26828872 - 07/17/20 12:23 PM (3 years, 6 months ago)

Quote:

roarkell said:
I've had some take 10+ days to germ... another thing I used to do when using swabs from syringe was I'd let the syringe sit in the sab, needle on, pointed down (usually just set it in a pint jar) overnight... then do your drops to swab(s)... whatever solids (hopefully spores) are in your solutions should sink down towards the needle.



Yeah, when I filtered the water throught the q-tip cotton, I filtered about 3-4 cc's through and there was some visible darkness on the cotton so I think I should have got a sufficient amount for germination and growth.  It's just discouraging seeing zero growth after about nine days.  I think the mycelium in the picture is contamination.


Edited by mysticalshroom975 (07/17/20 12:28 PM)


Extras: Filter Print Post Top
Offlineroarkell
The Roar Master!
Male User Gallery


Registered: 08/29/19
Posts: 2,223
Loc: Southern WI Flag
Last seen: 6 months, 21 days
Re: Nonviable spore syringes? Agar issues. [Re: mysticalshroom975]
    #26829061 - 07/17/20 01:45 PM (3 years, 6 months ago)

Whatever is going on in those pics doesnt look too promising... but that's why we use agar... at least your not shooting syringes straight to grain... lol. The only other thing I could suggest would be shoot me a PM  and I can shoot you some prints... I've got plenty of variety if you wanna check my trade list. Working from a print is far easier IME.


Extras: Filter Print Post Top
Offlinejcm4620
Stranger
 User Gallery

Registered: 05/26/19
Posts: 6,700
Last seen: 1 year, 11 months
Trusted Cultivator
Re: Nonviable spore syringes? Agar issues. [Re: roarkell]
    #26829098 - 07/17/20 02:03 PM (3 years, 6 months ago)

well i got as far as the word redit then i stopped reading. but honestly man u should stay far fat away from that god awful place. those ppl there are complete hacks. that place is by far the worst place to get any kind of advice from. you are at the correct place now and i suggest you erase everything that you read or got told from there from your memory and start some fresh research here. and im sure someone will gladly send u a print. i know i would but all i have at the moment are pans


--------------------
PANAEOLUS FRUITING MADE SIMPLE



Extras: Filter Print Post Top
InvisibleTormatoMFacebookDiscordReddit
The Goddess Kali Meh 😛
I'm a teapot User Gallery


Folding@home Statistics
Registered: 07/01/17
Posts: 6,067
Trusted Cultivator
Re: Nonviable spore syringes? Agar issues. [Re: jcm4620]
    #26829113 - 07/17/20 02:16 PM (3 years, 6 months ago)

Quote:

jcm4620 said:
well i got as far as the word redit then i stopped reading. but honestly man u should stay far fat away from that god awful place. those ppl there are complete hacks. that place is by far the worst place to get any kind of advice from. you are at the correct place now and i suggest you erase everything that you read or got told from there from your memory and start some fresh research here. and im sure someone will gladly send u a print. i know i would but all i have at the moment are pans



I second this....Reddit is a terrible source for Cultivation information. :thumbdown:


--------------------
Helpful Threads

The Shroomery Store

Tormato's Q&A Thread Post Questions Here or PM me!

"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead

Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!


Extras: Filter Print Post Top
Offlineroarkell
The Roar Master!
Male User Gallery


Registered: 08/29/19
Posts: 2,223
Loc: Southern WI Flag
Last seen: 6 months, 21 days
Re: Nonviable spore syringes? Agar issues. [Re: Tormato]
    #26829158 - 07/17/20 02:35 PM (3 years, 6 months ago)

Haha... I wasnt gonna knock reddit but when back to back TCs chime in, you know what's up... lol. Seriously like jcm said, check around the shroomery, theres a lot of super generous people here.


Extras: Filter Print Post Top
Offlineredhandmat
Dude


Registered: 05/09/19
Posts: 463
Last seen: 2 years, 8 months
Re: Nonviable spore syringes? Agar issues. [Re: roarkell]
    #26829184 - 07/17/20 02:50 PM (3 years, 6 months ago)

Quote:

roarkell said:
Whatever is going on in those pics doesnt look too promising... but that's why we use agar... at least your not shooting syringes straight to grain... lol. The only other thing I could suggest would be shoot me a PM  and I can shoot you some prints... I've got plenty of variety if you wanna check my trade list. Working from a print is far easier IME.




This guy right here is a savior, and I should know!


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: PhytoExtractum Buy Bali Kratom Powder   Left Coast Kratom Buy Kratom Capsules   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures   Kraken Kratom Red Vein Kratom   North Spore Injection Grain Bag


Similar ThreadsPosterViewsRepliesLast post
* Spores on agar question .More info plz tripndicular 4,345 5 09/13/02 05:27 PM
by tripndicular
* spore syring is in trouble? mulan 14,122 12 08/29/09 09:15 PM
by HaRo On KoRn
* Spore Syring Cont/Clear Misconcept if any Zwieback0 5,674 4 08/11/08 03:22 AM
by fr33d0mfry
* Re: When buying a spore syring... 3Mshroom 7,932 2 06/01/00 08:17 PM
by 3Mshroom
* Re: Oven innoculation may have killed my spores! Anonymous 2,272 16 04/12/00 03:37 PM
by oOjonahOo
* Benefits of Agar? celsius 9,381 10 12/01/19 12:36 AM
by wildernessjunkie
* Re: Spore transfer? Anonymous 1,901 1 03/08/01 08:17 PM
by Juggles
* how far to spore prints go? AKA PC 3,737 11 01/28/03 08:07 AM
by debianlinux

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
275 topic views. 31 members, 192 guests and 35 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.028 seconds spending 0.009 seconds on 14 queries.