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Offlinemysticalshroom975
Stranger


Registered: 07/03/20
Posts: 78
Last seen: 9 months, 20 days
Contamination or weak mycellium?
    #26826233 - 07/16/20 02:08 AM (3 years, 6 months ago)

I've been having difficulty producing mycellium from my spore syringe.  There was only one spot that produced any mycellium.  I isolated it and transferred to another plate.  It looks super whipsy, thin cotton like.  Smells kinda musty/mold like (but I don't have the best nose).  Here's a few pictures.  What are your thoughts?  Contamination or weak mycelium?  Perhaps I should start over? 


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Offlinehaschpapi
new kid

Registered: 12/27/18
Posts: 4
Loc: information age
Last seen: 11 months, 20 days
Re: Contamination or weak mycellium? [Re: mysticalshroom975]
    #26826247 - 07/16/20 02:20 AM (3 years, 6 months ago)

how old are your plates? if the white stuff appeared like a day after transferring, i would be worried. however if it took a few days to grow out like this it is likely just tomentose mycelium.


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Offlinemysticalshroom975
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Registered: 07/03/20
Posts: 78
Last seen: 9 months, 20 days
Re: Contamination or weak mycellium? [Re: haschpapi]
    #26826258 - 07/16/20 02:31 AM (3 years, 6 months ago)

Maybe after about a week.  At first the agar I used was made with this ratio: 20g potato starch, 25g maple syrup, 20g agar, 1 L water.  Then I transfered it to malt agar plates: 25g malt, 20g agar, 1L water (shown in the pic after about five days of growth at 80F).  This was the only mycilium I was able to get to grow on the potato plates, three plates total.  The first one had an accidental large splurt of syringe water while the other two only had ~3 drops from a syringe (that I couldn't see any spores or darkness) and yielded nothing so far except potentially bacterial contamination.


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Offlinemysticalshroom975
Stranger


Registered: 07/03/20
Posts: 78
Last seen: 9 months, 20 days
Re: Contamination or weak mycellium? [Re: haschpapi]
    #26826260 - 07/16/20 02:33 AM (3 years, 6 months ago)

Also, it should be noted the mycilium has the consistency of dried soap bubbles.  Like if you take a bath, make a lot of bubbles, then allow the bubbles to dry.  If you know what I'm talking about...


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Offlinewtfcrazymofo
foil hater
Other


Registered: 07/26/15
Posts: 1,204
Loc: Colonial alley
Last seen: 6 hours, 3 minutes
Re: Contamination or weak mycellium? [Re: mysticalshroom975]
    #26826291 - 07/16/20 03:13 AM (3 years, 6 months ago)

It does look like dried soap on agar.  How did you sniff that one?


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Offlinemysticalshroom975
Stranger


Registered: 07/03/20
Posts: 78
Last seen: 9 months, 20 days
Re: Contamination or weak mycellium? [Re: wtfcrazymofo]
    #26828020 - 07/17/20 12:00 AM (3 years, 6 months ago)

I just carefully smelled it.  I don't know what it's supposed to smell like so I don't have anything to reference to.  I recall it smelled musty/moldy.


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