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metaphoric
Mr. Bater



Registered: 05/18/15
Posts: 730
Last seen: 2 years, 3 months
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The double tap (double shake tek)
#26703566 - 05/29/20 06:13 AM (3 years, 8 months ago) |
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Hey guys, I've been using a lot of MS lately and I've been experimenting with the best time to shake and wanted to share an interesting find. This isn't really applicable for most of you pros since its useless for g2g jars but anyone using small MS squirts or single agar wedges might like this.
Basically you shake at ~10%, wait 4-5 days and shake again.
Try it for yourself in a side by side comparison. You'll be surprised by the speed!
My first shake is when the myc is about the size of two dimes, very early. 5 days later each colonised grain has touched all its surrounding grains, even if it hasn't enveloped the new grains no worries, the myc only has to reach out and touch them. Now your points of inoculation have increased x8 or x10. That's when it gets its second shake and its done in less than a week.
Its still not faster than g2g or LC of course, but it will take a week (at least) off of your total colonization time if you're using some form of single point inoculation like MS syringe or agar.
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 BEHOLD 
        
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General_Interest
Dandy



Registered: 05/11/20
Posts: 24
Loc: Montreal
Last seen: 3 years, 2 months
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Re: The double tap (double shake tek) [Re: metaphoric]
#26821232 - 07/13/20 02:17 PM (3 years, 7 months ago) |
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Thank you for sharing this! I have been using some pretty small agar wedges because pastyplates, glad to see somebody with a little more experience sharing this easy technique.
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mycologi.st
Stranger
Registered: 07/15/20
Posts: 8
Last seen: 3 years, 3 months
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I've been doing something similar when I smash up an agar plate in a bag and make sure it's well distributed. Works nicely
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AlexLPD
Stranger


Registered: 07/16/20
Posts: 2
Last seen: 3 years, 1 month
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Re: The double tap (double shake tek) [Re: mycologi.st]
#26827069 - 07/16/20 02:07 PM (3 years, 6 months ago) |
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Thanks for the share, I recently make an experiment when I dont shake on e of the jars, all of them become 100 Percent colonized, but this one dont, I open it and smells like vinegar.
So the next bath Im waithing to the 4th day of colonization and then shake it, I have one more shake on the 8 to 9th day, an yes, the things are going quite more faster.
Good vibes. -Alex.
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metaphoric
Mr. Bater



Registered: 05/18/15
Posts: 730
Last seen: 2 years, 3 months
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Re: The double tap (double shake tek) [Re: AlexLPD]
#26829563 - 07/17/20 06:32 PM (3 years, 6 months ago) |
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Quote:
AlexLPD said: Thanks for the share, I recently make an experiment when I dont shake on e of the jars, all of them become 100 Percent colonized, but this one dont, I open it and smells like vinegar.
So the next bath Im waithing to the 4th day of colonization and then shake it, I have one more shake on the 8 to 9th day, an yes, the things are going quite more faster.
Good vibes. -Alex.
This vinegar smelling issue is unrelated to your method of shaking. You are describing unhealthy spawn, I'd revisit your sterile tek.
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 BEHOLD 
        
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