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OfflineHex appeal
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Should I be doing an agar transfer?
    #26827018 - 07/16/20 01:37 PM (3 years, 6 months ago)

So I inoculated a few plates using about 1cc of a syringe. It wasnt until later I found out that a pretty aggressive amount. The good news is save for one plate they all seem to be healthy. My question is given the way they are all growing independently  should I transfer them? Will they integrate with each other or what? I took a picture here. Any insight is greatly appreciated!



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OnlineCamera93
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Re: Should I be doing an agar transfer? [Re: Hex appeal]
    #26827024 - 07/16/20 01:42 PM (3 years, 6 months ago)

yes start making transfers while you can still see individual colonies

that is some pretty good looking germination, I'd grab some edges of the rizo stuff and see how that T1 comes out


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InvisibleGoatrider
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Re: Should I be doing an agar transfer? [Re: Camera93]
    #26827093 - 07/16/20 02:22 PM (3 years, 6 months ago)

Yeah, that looks very promising.


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OfflineHex appeal
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Re: Should I be doing an agar transfer? [Re: Goatrider]
    #26827135 - 07/16/20 02:45 PM (3 years, 6 months ago)

Thanks for the responses! It's my first go around so I'm actually super pumped everything so far is going well. When I'm transferring however should I be grabbing each individual little circlet of mycelium to it's own plate or should I grab a few and transfer to one plate. And what should I do with the plate I'm cutting the samplea from?


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Offlineredhandmat
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Re: Should I be doing an agar transfer? [Re: Hex appeal]
    #26827152 - 07/16/20 02:52 PM (3 years, 6 months ago)

Quote:

Hex appeal said:
Thanks for the responses! It's my first go around so I'm actually super pumped everything so far is going well. When I'm transferring however should I be grabbing each individual little circlet of mycelium to it's own plate or should I grab a few and transfer to one plate. And what should I do with the plate I'm cutting the samplea from?




Honestly I avoid transferring multiple colonies to same plate. Doesn't seem right to me. I like to see the entire grow, and not accidentally transfer contams with clean. Also dont transfer the entire little circlet as you called it. Only a tiny (rice corn size) bit of the best looking part from the leading edge. Personally I would take some of the rhizo and maybe a couple of the best looking tomentose too.

If you're still unsure of what I mean you can say so so we clarify further.


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OfflineHex appeal
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Re: Should I be doing an agar transfer? [Re: redhandmat]
    #26827172 - 07/16/20 03:02 PM (3 years, 6 months ago)

I am a bit unsure. I do feel like I did a fair amount of research before I started this endeavor however some of these terms I dont recognize. Well, namely "rhizo" and also what characteristics do i look for to deem it the best part of the mycelium? And once I've transferred these cut outs should I continue to let the mycelium grow or should I just add the remainder of the plate into a grain spawn? And thank you for taking the time to reply! I greatly appreciate the insight!


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Offlinew00tmycelium
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Re: Should I be doing an agar transfer? [Re: redhandmat]
    #26827175 - 07/16/20 03:03 PM (3 years, 6 months ago)

Based on your pictures, I usually wait a little bit longer to see the colonies, then I start cutting sections off around the edges.  You've got good germination but I also have seen good germination not amount to much.  I'd give it a day to see how they grow at that point, then start making some transfers.


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OfflineCiv
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Re: Should I be doing an agar transfer? [Re: redhandmat]
    #26827177 - 07/16/20 03:04 PM (3 years, 6 months ago)

Yeahm best to just go out and do use a separate plate for each transfer.


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Offlinew00tmycelium
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Re: Should I be doing an agar transfer? [Re: Hex appeal]
    #26827180 - 07/16/20 03:06 PM (3 years, 6 months ago)

Quote:

Hex appeal said:
I am a bit unsure. I do feel like I did a fair amount of research before I started this endeavor however some of these terms I dont recognize. Well, namely "rhizo" and also what characteristics do i look for to deem it the best part of the mycelium? And once I've transferred these cut outs should I continue to let the mycelium grow or should I just add the remainder of the plate into a grain spawn? And thank you for taking the time to reply! I greatly appreciate the insight!




You should check my post history, I have a lot of examples of rhizomorphic and tomentose on the same plates at the same time from the same cultures.  In my experience it really doesn't even matter much but the rhizo cultures do seem to be stronger and faster in general.  I'm on T3 plates right now that I just posted, cut out of entirely rhizo cultures.  The pictures describe it all.

EDIT:



Here's a quick edit to show one of the rhizo's I just transferred.  This was a T2 plate but the same rhizo exists on T1 that I've seen.


Edited by w00tmycelium (07/16/20 03:10 PM)


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OfflineSporestain
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Re: Should I be doing an agar transfer? [Re: Camera93]
    #26827205 - 07/16/20 03:21 PM (3 years, 6 months ago)

Awesome 👍 I've never had this happen I would've thought it was a weird contamination.


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OfflineHex appeal
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Re: Should I be doing an agar transfer? [Re: w00tmycelium]
    #26827286 - 07/16/20 03:59 PM (3 years, 6 months ago)

[Quote]You should check my post history, I have a lot of examples of rhizomorphic and tomentose on the same plates at the same time from the same cultures.  In my experience it really doesn't even matter much but the rhizo cultures do seem to be stronger and faster in general.  I'm on T3 plates right now that I just posted, cut out of entirely rhizo cultures.  The pictures describe it all.

EDIT:



Here's a quick edit to show one of the rhizo's I just transferred.  This was a T2 plate but the same rhizo exists on T1 that I've seen.





Thank you I will certainly go through them. Any links you find to be particularly helpful I would appreciate. So I notice (or at least it seems to me) you took 4 different samples and transferred them to the one plate. Is that typical? And I really am still concerned with what I do with the remaining mycelium  from the plate I'm taking samples from as there will definitely be leftover mycelium  there. Thank you again though this has been super helpful so far and I plan to do some transfers tonight. I just want to have a little more information before I do so.


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Offlinew00tmycelium
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Re: Should I be doing an agar transfer? [Re: Hex appeal]
    #26827313 - 07/16/20 04:09 PM (3 years, 6 months ago)

Probably not normal at all, I was just experimenting with transfers... as in which chunks would be the most viable.  On my T3 plates recently I am only doing strong colonies, one per plate, just to see how it goes.

But you should also do the same... I've had really good results by putting four small chunks on the plate in each corner just to see which ones survive the most and then clone from there.

EDIT: You don't have anything to lose by slicing up any culture and putting them anywhere on a plate to see what they do.


Edited by w00tmycelium (07/16/20 04:10 PM)


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OfflineJohn in WI
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Re: Should I be doing an agar transfer? [Re: Camera93]
    #26827444 - 07/16/20 05:13 PM (3 years, 6 months ago)

Quote:

Camera93 said:
yes start making transfers while you can still see individual colonies

that is some pretty good looking germination, I'd grab some edges of the rizo stuff and see how that T1 comes out





I agree with this.  I haven't used a spore syringe in years. I do spore prints to agar, and the spores germinate making a real mess.  If you can pinch sections off of individual colonies, you're a couple steps ahead.


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InvisibleRoger Clemency
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Re: Should I be doing an agar transfer? [Re: John in WI]
    #26827754 - 07/16/20 08:11 PM (3 years, 6 months ago)

The idea with agar is to germinate spores, then transfer away from a few spots to a couple new plates by cutting a small wedge of agar at the leading edge of your myc and plopping that in the center of a clean plate.

You transfer because spore prints can often have mold spores in them and you cant always see it when they first germinate because its chaotic. Once you transfer it you let it grow out and see if it's clean. If there is something bad growing you would cut out a little wedge of a clear area and transfer that to a new plate.

It doesn't matter usually what happens to the plates you transfer from but if you save them for a while and they're mostly clean they will pin and you can clone those pins.

This is good stuff about agar https://www.shroomery.org/forums/showflat.php/Number/18430998/fpart/1/vc/1

I would let those plates grow out a little bit more before transferring. 


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Offlinew00tmycelium
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Re: Should I be doing an agar transfer? [Re: Roger Clemency]
    #26827909 - 07/16/20 09:55 PM (3 years, 6 months ago)

Quote:

Roger Clemency said:
The idea with agar is to germinate spores, then transfer away from a few spots to a couple new plates by cutting a small wedge of agar at the leading edge of your myc and plopping that in the center of a clean plate.

You transfer because spore prints can often have mold spores in them and you cant always see it when they first germinate because its chaotic. Once you transfer it you let it grow out and see if it's clean. If there is something bad growing you would cut out a little wedge of a clear area and transfer that to a new plate.

It doesn't matter usually what happens to the plates you transfer from but if you save them for a while and they're mostly clean they will pin and you can clone those pins.

This is good stuff about agar https://www.shroomery.org/forums/showflat.php/Number/18430998/fpart/1/vc/1

I would let those plates grow out a little bit more before transferring. 




This is pretty useful, because I've never had contamination before but I can see how that might be a huge problem for a new grower.  Also being able to identify the contams, and isolating the culture too.


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Offlinew00tmycelium
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Re: Should I be doing an agar transfer? [Re: w00tmycelium]
    #26830089 - 07/18/20 01:57 AM (3 years, 6 months ago)

Reply to this:



I had a super saturated syringe from an ebay purchase, and you can even see all the clumps on this plate. 10+ days later and absolutely nothing anywhere, I doubt it will do anything.

Same plates/substrate that are doing great with every other spores.  But visible spores apparently mean nothing.


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Offlineredhandmat
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Re: Should I be doing an agar transfer? [Re: w00tmycelium]
    #26830196 - 07/18/20 05:16 AM (3 years, 6 months ago)

Quote:

w00tmycelium said:
Reply to this:



I had a super saturated syringe from an ebay purchase, and you can even see all the clumps on this plate. 10+ days later and absolutely nothing anywhere, I doubt it will do anything.

Same plates/substrate that are doing great with every other spores.  But visible spores apparently mean nothing.




Thats sooo bacterial though. Looks nasty. How much did you squeeze out? A tiny drop should be enough.


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Offlinew00tmycelium
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Re: Should I be doing an agar transfer? [Re: redhandmat]
    #26830199 - 07/18/20 05:23 AM (3 years, 6 months ago)

Quote:

redhandmat said:

Thats sooo bacterial though. Looks nasty. How much did you squeeze out? A tiny drop should be enough.




That's the thing, there's nothing growing on it period (on the plate), not even bacteria.  I'm kinda shocked.  Which I also took the same thing and sprayed it into really good grains just to see if it would do anything there either -- still nothing (same grains that are doing very well with everything else).

I've had contam'd syringes before plenty of times, but this thing is just dead on anything.  Time to toss it all in the trash.


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Offlineredhandmat
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Re: Should I be doing an agar transfer? [Re: w00tmycelium]
    #26830207 - 07/18/20 05:40 AM (3 years, 6 months ago)

You can see that that plate is transparent in some areas and not in others right?


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OfflineMatty_Rulez
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Re: Should I be doing an agar transfer? [Re: w00tmycelium]
    #26830223 - 07/18/20 06:03 AM (3 years, 6 months ago)

Yeah..  that is super bacterial..  bad syringe, perhaps?  As said above, just I tiny drop is needed.


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