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n0bel
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Registered: 04/06/14
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I've been improving my agar game and finally have a bunch of plates great clean rhizo growth. How do I not screw it up?
#26826250 - 07/16/20 02:24 AM (3 years, 6 months ago) |
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I've now got a lot of agar plates with clean rhizomorphic growth after several transfers. Normally I've found a way to work from MS syringes direct injected into grains with mixed success. I've never had so much clean myc to transfer. How do I not fuck this up? Should I just tiger drop into a standard grain spawn? Are there any tek's I'm missing?
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mushpunx
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Registered: 04/20/14
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Re: I've been improving my agar game and finally have a bunch of plates great clean rhizo growth. How do I not screw it up? [Re: n0bel]
#26826301 - 07/16/20 03:24 AM (3 years, 6 months ago) |
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Good move working with agar
What's next is pretty easy. I usually keep a plate of my culture around, so if you don't have one, you might want to make a transfer to a plate you can grow out (2/3rds of the plate) and put in the cold storage.
Inoculating grain masters with your agar culture is pretty easy. It's basically the same motions as making a transfer from plate to plate.
1. Wipe down your receiving jar + loosen the lid some so you can open it with one hand.
2. Flame your scalpel + lift plate lid with one hand, cut + lift out your wedge (I like to use 1/4 plate per quart jar but you can use more/less), set lid back down on plate. I like to open my plate,cut it into quarters + replace the lid before hand so all I have to do is open the lid + pick the wedge up.
3. Lift lid on receiving jar, drop wedge in, close lid. Label jar.
That's it! I don't shake my wedge in to the grain, I just drop it in on top + let it settle a little. You can shake it in to the grains if you want - this is just the basic steps everybody has their own preferences.
If you can make clean agar transfers you can do this. Also you don't really have to worry about whether the myc is rhizomorphic or tomentose, neither indicates how the culture will perform. Just look for strong + clean looking growth over weak growth. Once you have a clean culture put it to grain.
--------------------
 Amateur Mycologists United AMU Q&A
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n0bel
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Registered: 04/06/14
Posts: 324
Loc: United States
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Re: I've been improving my agar game and finally have a bunch of plates great clean rhizo growth. How do I not screw it up? [Re: mushpunx]
#26826341 - 07/16/20 04:57 AM (3 years, 6 months ago) |
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So basically tiger drop into a big grain jar??
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 10 days, 16 hours
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Re: I've been improving my agar game and finally have a bunch of plates great clean rhizo growth. How do I not screw it up? [Re: n0bel]
#26826345 - 07/16/20 05:03 AM (3 years, 6 months ago) |
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Quote:
n0bel said: So basically tiger drop into a big grain jar??
You just cut a wedge out with a scalpel + drop it into the receiving jar.
I think Tiger drop is where you flip the agar plate (in the Tek it's a PastyPlate) upside down over the mouth of the receiving jar to drop the whole plate in.
Edited by mushpunx (07/16/20 05:12 AM)
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Roger Clemency
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Registered: 03/23/20
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Re: I've been improving my agar game and finally have a bunch of plates great clean rhizo growth. How do I not screw it up? [Re: mushpunx]
#26826407 - 07/16/20 07:03 AM (3 years, 6 months ago) |
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I like Munchauzen’s Blenderless LI method - https://www.shroomery.org/forums/showflat.php/Number/22833314/fpart/1/vc/1
I’d drop a few wedges but also try the LI. Jars colonize in about 10 days, it’s great.
-------------------- Sour grapes, sweet revenge Heaven starts right where hell ends
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