Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Bridgetown Botanicals CBD Concentrates   Mushroom-Hut Substrate Bags   PhytoExtractum Kratom Powder for Sale   Left Coast Kratom Buy Kratom Extract

Jump to first unread post Pages: 1
Offlinethesupersoap33
curious george
 User Gallery


Registered: 06/28/16
Posts: 338
Last seen: 3 years, 6 months
Contams on pasty plates 3 weeks after innoc...
    #26823332 - 07/14/20 03:29 PM (3 years, 6 months ago)

I've been growing cultures for several weeks now and I'm on my 4th set of past plates. The third set is showing signs of contams 3 weeks in while the second set didnt have hardly any before I trashed them. I dont want to start a master jar until I'm certain it's going to be contam free.

Started from spores on one, then a mycelium extraction the next 3.


Extras: Filter Print Post Top
OfflineTrichodingus
Stranger


Registered: 05/31/20
Posts: 85
Loc: Communing with the divine
Last seen: 3 years, 6 months
Re: Contams on pasty plates 3 weeks after innoc... [Re: thesupersoap33]
    #26823353 - 07/14/20 03:44 PM (3 years, 6 months ago)

Are you transferring the good myc onto fresh plates for the 4th time or starting from spores for the 4th time?


Extras: Filter Print Post Top
Offlinethesupersoap33
curious george
 User Gallery


Registered: 06/28/16
Posts: 338
Last seen: 3 years, 6 months
Re: Contams on pasty plates 3 weeks after innoc... [Re: Trichodingus]
    #26823670 - 07/14/20 06:22 PM (3 years, 6 months ago)

Fresh myc from the new plates. I cut out around the edges.


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: Contams on pasty plates 3 weeks after innoc... [Re: thesupersoap33]
    #26823706 - 07/14/20 06:45 PM (3 years, 6 months ago)

What kind of contams are we talking about? Meshed in mold? Mold satellites? Bacteria sliding around the plate riding with the colony? Different contams have different vectors and different ways to clean up so knowing the general taxonomy of what your dealing with would be helpful.


Extras: Filter Print Post Top
Offlinethesupersoap33
curious george
 User Gallery


Registered: 06/28/16
Posts: 338
Last seen: 3 years, 6 months
Re: Contams on pasty plates 3 weeks after innoc... [Re: Pastywhyte]
    #26824209 - 07/15/20 12:33 AM (3 years, 6 months ago)

Just made the pic featuring contams my avatar. This is roughly 3 to 4 weeks after innoc. It was clean before the last four or five days.


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: Contams on pasty plates 3 weeks after innoc... [Re: thesupersoap33]
    #26824652 - 07/15/20 08:44 AM (3 years, 6 months ago)

Okay so you have mold riding along with the colony. That can be a real bitch. I wouldn’t pin my hopes on that colony, get more spores on to agar. The way to do it is knock up at least 4 or 5 plates with spores. Then only choose the best of those, toss the rest.


Extras: Filter Print Post Top
Offlinethesupersoap33
curious george
 User Gallery


Registered: 06/28/16
Posts: 338
Last seen: 3 years, 6 months
Re: Contams on pasty plates 3 weeks after innoc... [Re: Pastywhyte]
    #26824897 - 07/15/20 10:41 AM (3 years, 6 months ago)

That's sort of what I did. I knocked up 4 plates with spores initially... all from the same print.

Are you saying I should start over from scratch?

Thanks btw.


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: Contams on pasty plates 3 weeks after innoc... [Re: thesupersoap33]
    #26824914 - 07/15/20 10:51 AM (3 years, 6 months ago)

Yeah if you have meshed in mold it’s often not worth the effort to save the culture. I would only bother if it was a killer clone I was trying to save.


Extras: Filter Print Post Top
Offlinethesupersoap33
curious george
 User Gallery


Registered: 06/28/16
Posts: 338
Last seen: 3 years, 6 months
Re: Contams on pasty plates 3 weeks after innoc... [Re: Pastywhyte]
    #26825557 - 07/15/20 04:39 PM (3 years, 6 months ago)

Cool cool. I have no idea what the print is from. I did use cloth medical tape instead of micropore on the plates. Is that a potential vector for contamination? Also, I know I shouldn't have, but I wasnt using a clean box when i was making the transfers. My apt is super clean and it was during lockdown and i couldn't get to the store to get supplies to build a clean box.


Extras: Filter Print Post Top
Offlinegtshroom
McKen'ed
 User Gallery
Registered: 07/24/18
Posts: 64
Last seen: 6 months, 12 days
Re: Contams on pasty plates 3 weeks after innoc... [Re: Pastywhyte]
    #26825580 - 07/15/20 04:51 PM (3 years, 6 months ago)

Quote:

Pastywhyte said:
What kind of contams are we talking about? Meshed in mold? Mold satellites? Bacteria sliding around the plate riding with the colony? Different contams have different vectors and different ways to clean up so knowing the general taxonomy of what your dealing with would be helpful.





Does a tek exist which lists the different ways to clean up agar based on contamination type?

THX


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: Contams on pasty plates 3 weeks after innoc... [Re: gtshroom]
    #26825756 - 07/15/20 06:42 PM (3 years, 6 months ago)

Not for the most part. But for bacteria I like an agar sandwich. Meshed in mold I would try a warm pour. For yeast I would drop the temp to the mid 60’s. Antibiotics can provide an edge as well.

Agar sandwich tek

https://www.shroomery.org/forums/showflat.php/Number/2894662


Extras: Filter Print Post Top
Offlinethesupersoap33
curious george
 User Gallery


Registered: 06/28/16
Posts: 338
Last seen: 3 years, 6 months
Re: Contams on pasty plates 3 weeks after innoc... [Re: Pastywhyte]
    #26827513 - 07/16/20 05:56 PM (3 years, 6 months ago)

What about the duopore cloth tape for the breather hole on the plates? Is that not a good substitution for micropore tape?

None of the stores around me have micropore.


Extras: Filter Print Post Top
InvisibleRoger Clemency
Smile
 User Gallery

Registered: 03/23/20
Posts: 2,005
Re: Contams on pasty plates 3 weeks after innoc... [Re: thesupersoap33]
    #26827601 - 07/16/20 06:45 PM (3 years, 6 months ago)

I don't know about the tape but are you saying you're doing these transfers just in open air? If that's the case you have to get some kind of still air box. It's not cleanliness that you're looking for its just a small closed in area with zero air movement. Then if you don't let your hands hover above your work you'll be able to do sterile procedures.


--------------------
Sour grapes, sweet revenge
Heaven starts right where hell ends


Extras: Filter Print Post Top
OfflineDoyledozo
Humble student
Male


Registered: 10/22/19
Posts: 156
Last seen: 8 months, 6 days
Re: Contams on pasty plates 3 weeks after innoc... [Re: Roger Clemency]
    #26827689 - 07/16/20 07:32 PM (3 years, 6 months ago)

I've had regular Petris mold on the edges from using cloth tape. I'd stick with paper MP tape.


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Bridgetown Botanicals CBD Concentrates   Mushroom-Hut Substrate Bags   PhytoExtractum Kratom Powder for Sale   Left Coast Kratom Buy Kratom Extract


Similar ThreadsPosterViewsRepliesLast post
* rye jar has no results no contam(?) nothing 2 week nw2shroom 1,876 6 03/21/02 08:56 AM
by Seuss
* Contam or Metabolites? (BRF Jar) Cogutopia 218 5 07/16/20 03:23 PM
by Cogutopia
* contam probs Scourge 809 2 10/09/01 11:58 AM
by MAIA
* What is better? (innoc. ques) mahookah 1,336 12 10/20/01 11:35 PM
by tchyted
* Visual diff between contam and Myc. for newbie? Pachanguero 2,148 4 06/18/05 06:34 AM
by drugsaregood
* mycellia growth vs. contam angerman 931 1 04/14/02 01:04 PM
by phrozendata
* Anyone else have contam. probs. with Equador?
( 1 2 3 all )
Sick_Of_It_All 6,701 49 08/20/02 02:13 PM
by sirJ
* Contam free MEA upupup 1,538 3 12/12/02 12:28 PM
by upupup

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
227 topic views. 35 members, 197 guests and 72 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.026 seconds spending 0.007 seconds on 12 queries.