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placeboJesus
Explorer


Registered: 07/15/20
Posts: 2
Last seen: 3 years, 5 months
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Inoculation - First Time Grower
#26824824 - 07/15/20 10:09 AM (3 years, 6 months ago) |
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Heyo, I'm about to begin growing some PES Amazonian Cubensis. I'm using 16 oz/1 pint jars and was wondering how much of the liquid culture i should use per jar? I've heard that it's around 1.0-1.5 cc but I'm not sure if that is dependent on the size of the jar. Also, if y'all have any other advice for a first time grower I would love to hear. I'm just waiting on my vermiculite to arrive then I can start. Super Stoked!!
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CrashTest
Stay Learning


Registered: 06/21/20
Posts: 623
Last seen: 6 months, 29 days
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Re: Inoculation - First Time Grower [Re: placeboJesus]
#26824863 - 07/15/20 10:23 AM (3 years, 6 months ago) |
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Start with P.F. tek or with agar. Follow a proven tek from this forum to a T, use the search function but also have some helpful links in my signature
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this account is automated, any posts related to activities or advice thereof are strictly opinions from numerous online sites and are for informational purposes only - CrashTest2020
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CagedApe
Free



Registered: 04/19/20
Posts: 135
Loc: The Ether
Last seen: 1 year, 7 months
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Re: Inoculation - First Time Grower [Re: CrashTest]
#26824893 - 07/15/20 10:40 AM (3 years, 6 months ago) |
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What method are you using? BRF tek?
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pesa



Registered: 06/13/20
Posts: 599
Last seen: 3 minutes, 41 seconds
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Re: Inoculation - First Time Grower [Re: placeboJesus]
#26825046 - 07/15/20 12:01 PM (3 years, 6 months ago) |
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1. 16 oz/1 pint/473ml jars. 1.5cc to 2cc works better.
2. if you are going with pftek make sure they are wide mouth jars.
3. make a still air box SAB for inoculation and for other coming projects.
4. after inoculation put the jars in a place where temperature is between 22c to 27c. never go beyond 27c it invites contaims.
5. chose a tek and follow it.
here are few things to read while you wait for vermiculite to arrive. good luck
https://www.shroomery.org/forums/showflat.php/Number/8468474#8468474
https://www.shroomery.org/forums/showflat.php/Number/24179086
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CrashTest
Stay Learning


Registered: 06/21/20
Posts: 623
Last seen: 6 months, 29 days
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Re: Inoculation - First Time Grower [Re: CagedApe]
#26825343 - 07/15/20 02:13 PM (3 years, 6 months ago) |
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Quote:
CagedApe said: What method are you using? BRF tek?
Pf tek yes, inoculating sterile brf/verm jars with a multi spore syringe from a vendor
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this account is automated, any posts related to activities or advice thereof are strictly opinions from numerous online sites and are for informational purposes only - CrashTest2020
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 10 days, 17 hours
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Re: Inoculation - First Time Grower [Re: placeboJesus]
#26825373 - 07/15/20 02:25 PM (3 years, 6 months ago) |
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Quote:
placeboJesus said: Heyo, I'm about to begin growing some PES Amazonian Cubensis. I'm using 16 oz/1 pint jars and was wondering how much of the liquid culture i should use per jar? I've heard that it's around 1.0-1.5 cc but I'm not sure if that is dependent on the size of the jar. Also, if y'all have any other advice for a first time grower I would love to hear. I'm just waiting on my vermiculite to arrive then I can start. Super Stoked!!
1. Are you inoculating BRF cakes or grain?
2. How did you make your liquid culture? You don't mean spore syringe do you?
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 Amateur Mycologists United AMU Q&A
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 3 hours, 25 minutes
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Re: Inoculation - First Time Grower [Re: mushpunx]
#26825378 - 07/15/20 02:27 PM (3 years, 6 months ago) |
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Liquid culture is different from spore syringes.
I think people usually use .5-1cc per hole in the jar with spores. Basically a drop or two. As little as possible.
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LAGM2020     
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Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: Inoculation - First Time Grower [Re: CrashTest]
#26825385 - 07/15/20 02:28 PM (3 years, 6 months ago) |
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1CC of spore solution per jar is enough, you're looking to inject a minimum amount of bacterial spore solution inside the substrate, 0.25CC per inoculation spot is enough for germination. Full pints are not optimal but doable, I'll assume you knew that. Just don't tilt and move the jars around after you inoculate, there won't be need to do anything to the jars for the coming 2-3 weeks after inoculation. Gl
-------------------- Cakes inside Water Tub
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 10 days, 17 hours
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Re: Inoculation - First Time Grower [Re: A.k.a]
#26825400 - 07/15/20 02:33 PM (3 years, 6 months ago) |
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Yeah I'm thinking he means spore solution instead of liquid culture but :shrug;
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 Amateur Mycologists United AMU Q&A
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placeboJesus
Explorer



Registered: 07/15/20
Posts: 2
Last seen: 3 years, 5 months
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Re: Inoculation - First Time Grower [Re: placeboJesus]
#26825607 - 07/15/20 05:05 PM (3 years, 6 months ago) |
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Sorry yes, I am using the BRF and I meant spore syringes instead of liquid cultures. Thank you guys so much!
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