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Offlinemato4242
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Registered: 09/27/18
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Last seen: 3 years, 8 days
Strange transparent thing on my agar (could be bacterial)
    #26824738 - 07/15/20 09:29 AM (3 years, 6 months ago)

Hey guys,

for the past half a year or even more, I am having trouble with bacteria or so it seems. Everytime I start a new culture on agar plates (or even by cloning on agar) I get this strange slimmy and transparrent looking thing around my mycelium. It doesn't even have to be that slimmy acctually, sometimes it's just a shiny thing. The thing is only visible on the outer edges of mycelium. It looks as though it is strangling the myc, alot of times preventing it to grow. Most of the time, eventually it grows out and I can transfer some myc to new plates, but the problem reocurrs. And when I got to the stage that the myc on the agar plates looks decent enough to put it to grains, the grains turn out to be slimy looking again at the end right when the jars are done. I have spawned those jars and sometimes it wouldn't even colonize the whole substrate. Most of the time it would but it would still get mold before first flush.

Which led to my conclusion that it is bacterial. All fine. Then I took actions... I ordered spores and syringes from multiple viable sources, I made some spore prints using boddhi's second print technique, I bought presterile plastic petris and used those (I thought the glass ones didn't sterilize or have too much of imperfection on the two contact surfaces), I varied the nutrients on agar, varied the PC time... I even tried the biopsy method that I found here on the forum. With no success it seems. I am still going to spawn some jars, but it looks that the problem is here to stay.

I have the 23qt presto canner and cooker. I PC agar for at least 30 minutes. I did 45 also. I also properly vent for 10 mins. I am doing all the work in my big 130 liter SAB. I wipe all the walls and things that go in with 70% iso. I do not hoover my hands over opened things. I flame sterilize everything till red hot. I wear a mask.

That being said it is very strange that I very rarely get mold contams or even any other visible bacterial contam that is not radiating from the myc origin. So it seems to me the contam is from the source itself. But like I said I have used multiple sources, including clones.

After more than six months of battling and 200 petris wasted, I am turning to you guys to help me out and give me some clue. Would be very much appreciated.

Thanks guys!









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