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OfflineCrashTest
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CrashTest PF Tek Grow
    #26823584 - 07/14/20 07:42 PM (2 months, 12 days ago)

Hey guys how’s it going. Fairly new member to the forums but I’ve known about Shroomery for over 10 years and had only 1 successful pk tek jar back then, and haven’t been able to get back into cultivating until now. :sun:

My goal for this is to update as time goes, and eventually I will move to agar.

Inoculated twelve 1/2 pint wide mouth brf/verm jars between 6/21-6/27 with a B+ MSS. I lost two due to some mold after a week, which I tossed, bleached, sterilized and reinoculated, and now four more are showing signs of bacteria. I attribute this to my poor sterile tek while learning.

These are the four funky/questionable jars,  I have them isolated, waiting for full colonization to mix with some coir and put outside. I will NOT be opening these jars inside



Question- is it safe to eat dried mushrooms if they grow outside from these bacterial cakes ?

—————————————————————————————————————


I’ve learned a lot and from what’s left was able to birth two cakes today after seeing full colonization on 7/8:eek:



I have two more jars that fully colonized 7/11,



And two more at 95%


I also inoculated 10 more brf/verm jars of APE MSS on 7/11 and you can see that starting to germinate here


I got a bunch of jars going.



Hoping to see my contam rate go down as well, As Ive improved on my sterile tek. I use a SAB and sterile procedure but it gets warm in my closet setup which is invitation for contams. usually I’m able to keep it around 78 though when it’s 90 outside.

Going to be utilizing Mateah’s HC Tek for BRF cakes using 5L tubs and slightly over hydrated coir, after a 20 hour soak I’ll put those first two into fruiting and post updates. Minimum two cakes per tub, for a better microclimate.

Any tips appreciated
Thanks guys


Edited by CrashTest (07/14/20 07:53 PM)


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InvisibleROTIFIER
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Re: CrashTest PF Tek Grow [Re: CrashTest]
    #26823744 - 07/14/20 09:03 PM (2 months, 12 days ago)

Hey CrashTest, good to see your getting a few good jars off to a start here, I would say more than likely it's the MSS, or you've answered your own with sterile proc, you definitely got to get your agar session going though, nothing like good clean practice, whether or not it's needed for pf-tek it just helps to have a clean culture to rule out contams there in the first place. 

You planning on cloning?

My two cents on fruits growing from bacterial sub would be if they grow and their not slimed up and look ok, dry n try or you can boil up and make a tea from them if there's enough

Good luck man and I'm gonna keep eye on your updates


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OfflineCrashTest
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Re: CrashTest PF Tek Grow [Re: ROTIFIER]
    #26823759 - 07/14/20 09:17 PM (2 months, 12 days ago)

Quote:

ROTIFIER said:
Hey CrashTest, good to see your getting a few good jars off to a start here, I would say more than likely it's the MSS, or you've answered your own with sterile proc, you definitely got to get your agar session going though, nothing like good clean practice, whether or not it's needed for pf-tek it just helps to have a clean culture to rule out contams there in the first place. 

You planning on cloning?

My two cents on fruits growing from bacterial sub would be if they grow and their not slimed up and look ok, dry n try or you can boil up and make a tea from them if there's enough

Good luck man and I'm gonna keep eye on your updates




Thanks dude, already got my media bottles and just waiting for my plates to arrive. I’m planning at minimum to get as many prints as I can, and hopefully have plates ready to clone. I don’t have a PC yet but I think I can do it in an instapot or steam sterilize, for a test run to see if it will work. If not I’ll invest in a PC sooner rather than later.



I actually was thinking about that, is it possible to clone a dried shroom?

As far as the bacterial jars go, as I stated I won’t open those inside at all, so most likely I’ll bring them outside with some coco coir and just crumble them up near my garden. Then I’ll make sure to clean myself before going back to my spawn closet


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OfflineCrashTest
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Re: CrashTest PF Tek Grow [Re: CrashTest]
    #26825448 - 07/15/20 05:03 PM (2 months, 11 days ago)

Well here we go, after about 20 hours of soak time, rolled in verm and set into the fruiting chamber/HC tek.

RH in the room is about 50%, after 10 mins in this tub the RH inside is already 85%, lid unlatched. I removed the small handles which left two holes at the top which I covered with micropore tape. I have issues with gnats because we have so many house plants and a bunch of roommates. I don’t know if I’ll ever be able to colonize a bulk substrate until I get a handle on the gnats.

Everything smelled awesome and I’m just going to leave it alone and see what happens.



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Offlinepesa
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Re: CrashTest PF Tek Grow [Re: CrashTest] * 1
    #26825467 - 07/15/20 05:19 PM (2 months, 11 days ago)

fresh cakes do have nice smell.wait till you open fruiting chamber the smell will be more amazing.


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InvisibleMateah
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Re: CrashTest PF Tek Grow [Re: CrashTest] * 1
    #26825498 - 07/15/20 05:51 PM (2 months, 11 days ago)

Quote:

CrashTest said:
I don’t know if I’ll ever be able to colonize a bulk substrate until I get a handle on the gnats.



Gnats can't invade your substrates if you have the lids on during colonization.
Also Idk how a few gnats would possibly mess up a colonizing substrate, never had that happen to me altho I've seen a few gnats over the years :super:
Agar as all liquids can be boiled/steam sterilized :thumbup:


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OfflineCrashTest
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Re: CrashTest PF Tek Grow [Re: Mateah]
    #26825501 - 07/15/20 05:55 PM (2 months, 11 days ago)

Quote:

Mateah said:
Quote:

CrashTest said:
I don’t know if I’ll ever be able to colonize a bulk substrate until I get a handle on the gnats.



Gnats can't invade your substrates if you have the lids on during colonization.
Also Idk how a few gnats would possibly mess up a colonizing substrate, never had that happen to me altho I've seen a few gnats over the years :super:
Agar as all liquids can be boiled/steam sterilized :thumbup:





Thanks dude, that boosts my confidence a bit. Also thanks for checking out my thread, your HC tek for BRF cakes was truly inspiring.

Have everything to start agar/pastyplates except for a PC. Can I steam sterilize these glad mini rounds ?


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InvisibleMateah
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Re: CrashTest PF Tek Grow [Re: CrashTest] * 1
    #26825517 - 07/15/20 06:13 PM (2 months, 11 days ago)

If the containers can handle boiling temperatures then yes you can steam sterilize the agar mix in those :thumbup:
And neat looking HC's :super:


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OfflineCrashTest
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Re: CrashTest PF Tek Grow [Re: Mateah]
    #26825767 - 07/15/20 08:47 PM (2 months, 11 days ago)

Quote:

Mateah said:
If the containers can handle boiling temperatures then yes you can steam sterilize the agar mix in those :thumbup:
And neat looking HC's :super:




Thank you dude that means a lot.:thumbup::heart:

I used pp5 glad mini rounds, 4g agar, 4g LME and 200mL of water to make 8 pasty plates that I steam sterilized for 45 mins. Probably could have halved that recipe. I’m gonna let them cool overnight and leave them wrapped in the pot until I can attempt to inoc with two different methods.

I have a bunch of MSS syringes and an inoculation loop I made from an old whisk. Also have a homemade blade made using a razor and a coat hanger. Followed some videos from the forum. Should be easy to flame sterilize. I also have a mushroom that was dried and gifted to me that is huge, with the stipe intact that I’m going to try to clone on agar

Wish me luck! Going to have to start getting a grain jar prepped so I can do some shoeboxes


Edited by CrashTest (07/15/20 08:50 PM)


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OfflineCrashTest
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Re: CrashTest PF Tek Grow [Re: CrashTest]
    #26827124 - 07/16/20 04:40 PM (2 months, 10 days ago)

About 24 hours since I took these out of the dunk and rolled in verm and placed in the HC. Getting about 95% RH in the tub, I flipped the lid for a little more FAE but it’s looking promising, let me know what y’all think, it smells great in there.

I haven’t misted at all but wondering if I should give the verm a mist



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Edited by CrashTest (07/16/20 04:42 PM)


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InvisibleMateah
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Re: CrashTest PF Tek Grow [Re: CrashTest] * 1
    #26827223 - 07/16/20 05:32 PM (2 months, 10 days ago)

Wouldn't hurt to let go of a few rounds of the spray bottle from at least 3 feet away, let the fine mist gently hover down towards the general location of the cakes as the fins mist lands lil here and lil there finding its way into the HC from practically all directions and angles. This technique guarantees gradual buildup of these tiny tiny marbles everywhere inside the HC, and don't be shy with the spray bottle I sometimes squeeze off 20-40 shots from 4-5 feet awsy while I stop to check on the cakes surface conditions every 5-6 sprays. of these so don't be check up on the surface conditions every 5-6 rounds to notice gradual buildup until you achieve what you view as desired conditions. don't aim specifically for the cakes, sometimes il mist all over except directly towards the cskes depending on what kind of micro climate I'm creating/maintaining. All I'm ever looking to create is those densely packed micro droplets, they should barely be visible to the naked eye except for upon closer look, this kind of surface conditions always produce great pinsets as long as I maintain those handsome conditions on top and around the cakes. Droplets should cover the entire cake but should NEVER break the surface tention of the colony on top, prolonged standing water on top will eventually soak the surface and may damage the myc beyond the ability of  to create to get absorbed and soak the surface  thus nor collecting on top as small pools or big droplets. Always should be that Hydro phobic resembling surface tention where thousands of micro droplets can rest close to one another but never merging into one another. Hope that makes some sense :thumbup:


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OfflineCrashTest
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Re: CrashTest PF Tek Grow [Re: Mateah]
    #26827246 - 07/16/20 05:44 PM (2 months, 10 days ago)

That makes perfect sense, and pretty much exactly what I did except about 20-30 fine mist sprays from about 2-3 ft away over the tub but not aiming for the cakes specifically. I took a closer look just now and I can see the tiniest of droplets suspended on the myc.

Will check again in about 12 hours for these droplets but 99% RH with the lid flipped and tiny gaps for FAE is pretty dope

Thanks again Mateah  :rockon:





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Edited by CrashTest (07/16/20 05:46 PM)


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Re: CrashTest PF Tek Grow [Re: CrashTest]
    #26829133 - 07/17/20 04:23 PM (2 months, 9 days ago)

Almost 2 days in the tub. Is that a knot? Everything still smells great, gave them another light mist, but 99% RH inside the tub. Since no one will ever know I’m doing this except for my lady, this is the only place I can share pics lol



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Re: CrashTest PF Tek Grow [Re: CrashTest]
    #26829218 - 07/17/20 05:02 PM (2 months, 9 days ago)

You know when a pianist is about to begin the concert and he's warming up his fingers and stretching and what not. That's what the cakes are doing now, they have meeting right now discussing technicalities and signing papers. I wish them gl on their new job on Monday :bongload:


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OfflineCrashTest
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Re: CrashTest PF Tek Grow [Re: Mateah]
    #26829278 - 07/17/20 05:42 PM (2 months, 9 days ago)

He’s getting ready! Think this was the fastest colonizing cake. Fully consolidated in 20 days. Just dunked 2 more cakes that were 100% and will fruit tomorrow in a second HC, the same way. I have one more cake that’s fully colonized/consolidated but I don’t want to put one cake in a HC. Maybe top fruit it in the jar? I have some coir that’s ready to go, maybe shred it and put it in a small Tupperware container with a lid ? I won’t have any jars ready to fruit with it for a couple of weeks.

Thanks for all your advice bro


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Re: CrashTest PF Tek Grow [Re: CrashTest]
    #26829331 - 07/17/20 06:05 PM (2 months, 9 days ago)

Is it just me or are those 1/4 pints? 1/2pints are a little taller unless it's just the photo's


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Re: CrashTest PF Tek Grow [Re: Tripglass420]
    #26829350 - 07/17/20 06:11 PM (2 months, 9 days ago)

Nah these are true 1/2 pint wide mouth jars, they’re 8oz. Also the tubs are 5L tubs



These are hard to come by. I ordered 24 and I think at least 6 of them came smashed/cracked lol. My grow will not be for getting the most yield. It’s more about learning every process on a smaller scale as anything I cultivate is strictly for personal use.

Now I have 5 jars that look bacterial, and an overgrown veggie garden outside that I used store bought mushroom compost for, originally. Planning to mix these 5 bacterial jars with coir and spread it near/in my garden. Anyone know if I can do this before they are 100% colonized or should I wait ? I will be bringing the jars outside and opening them outside


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Edited by CrashTest (07/17/20 06:14 PM)


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Re: CrashTest PF Tek Grow [Re: CrashTest]
    #26830969 - 07/18/20 05:02 PM (2 months, 8 days ago)

So I wasn’t going to wait for weeks for these bacterial jars to fully colonize so I crumbled them up with some prepped coir and threw it outside near my veggie garden with some leaves over the top of it. Not expecting much if anything.




Then I showered and changed my clothes and prepping more coir to fruit three more cakes. I’m going to top fruit one in a bag with coir casing just to try it out, and another HC with two more cakes.

Here’s an update on the first HC on day 3



Looks like it’s about to explode lol


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Re: CrashTest PF Tek Grow [Re: CrashTest]
    #26831142 - 07/18/20 06:43 PM (2 months, 8 days ago)

Just make sure the myc doesn't jump off the cake onto the wet coir, I let it happen once and the results werent good. Gl


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Re: CrashTest PF Tek Grow [Re: Mateah]
    #26831147 - 07/18/20 06:46 PM (2 months, 8 days ago)

Quote:

Mateah said:
Just make sure the myc doesn't jump off the cake onto the wet coir, I let it happen once and the results werent good. Gl





That looks like what is happening. Should I make it a bigger tinfoil circle ?


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