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OfflineShroomSage
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Registered: 07/13/20
Posts: 4
Last seen: 3 years, 6 months
Advice on Grain Spawn Duration
    #26822103 - 07/13/20 11:06 PM (3 years, 6 months ago)

Hey all,

This is my first attempt growing and my first post here so if I do anything wrong please let me know so I can do better next time :smile:

I am needing advice for how long I should expect my spores to colonize my grain spawn. The species I am using is Penis Envy Uncut and I am using 2 bags of sterilized rye berry spawn with the ports. I put about 5cc's of PEU into each bag. I have a room dedicated to this and it is very well sterilized. I put the spores in on the 5th of July around 6pm(This is probably irrelevant but I am keeping a log).

So it has roughly been 9 days. I can see a bit of moisture in both bags mostly at the top of the grain, but I don't see much mycelium(I think this is correct?) growth. I think I see a bit if white stuff forming but it's so minimal I can't be for certain. I was curious how long I should wait before deeming this a failed attempt or am I just not being patient enough. Also advice on what to do better or what to look out for would be appreciated. Some more info I keep my house between 70-75 degrees.

Thanks!


Edited by ShroomSage (07/13/20 11:07 PM)


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OfflineSpirit-Crusher
DOOM HIPPIE


Registered: 04/24/19
Posts: 358
Loc: PNW
Last seen: 15 days, 14 hours
Re: Advice on Grain Spawn Duration [Re: ShroomSage]
    #26822120 - 07/13/20 11:16 PM (3 years, 6 months ago)

My advice is that you shouldn't inject spores into a spawn bag. But best of luck to you, just dont get your hopes up. If you are lucky enough I would say its going to take at least a month, especially that variety.


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OfflineShroomSage
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Registered: 07/13/20
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Last seen: 3 years, 6 months
Re: Advice on Grain Spawn Duration [Re: Spirit-Crusher]
    #26822122 - 07/13/20 11:18 PM (3 years, 6 months ago)

Thank you for your response. Next time I will seek a better option. As for now as long as hopes don't seem to be lost I will stick with it. <3


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OfflineRedWolf77
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Registered: 04/11/20
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Re: Advice on Grain Spawn Duration [Re: ShroomSage]
    #26822171 - 07/13/20 11:50 PM (3 years, 6 months ago)

In my somewhat limited experience, I'd say your okay. I inoculated sterile rye grain with B+ spore. I would check on them every few days and, like you, I was convinced that my spores were crap or my grain was no good. I had them inside a plastic tote at about room temp. I ordered more spores and kinda just forgot about these grain bags.
Then about 3 weeks later I just decided to look and Low & behold the bags were about %40-%50 colonized. So, I think you should just put em out of sight, out of mind for a while. I know your anxious and exited but you know what they say about mushroom growin'? Patience is the biggest thing.
Also, I found that a little higher temp than room temp & making sure they had a good fresh air infusion once a day made the mycelium colonize a good bit quicker.
You should consider trying agar and/or live mycelium liquid culture. It makes colonizing grain or whatever WAY quicker as you don't have to wait for spore germination.
Hope this is of some help.
good luck man


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REDWOLF


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OfflineShroomSage
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Registered: 07/13/20
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Last seen: 3 years, 6 months
Re: Advice on Grain Spawn Duration [Re: RedWolf77]
    #26822198 - 07/14/20 12:15 AM (3 years, 6 months ago)

Thank you so much for this! Definitely gave me more hope! I know the species I chose apparently takes a bit longer so on top of the above I think I'll just be patient and relax a bit. Like I noted I an keeping a log of everything so maybe after everything is all said and done I could make a post about my findings. Again thank you hope restored!


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OfflineManifoldPrime
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I'm a teapot


Registered: 03/16/17
Posts: 1,313
Loc: South Africa Flag
Last seen: 1 year, 1 month
Re: Advice on Grain Spawn Duration [Re: ShroomSage]
    #26822230 - 07/14/20 12:58 AM (3 years, 6 months ago)

Obligatory "spores on grain are risky" disclaimer.

It can take longer than 2 weeks for spores to germinate, and since bags are a big volume, it can also theoretically germinate inside the mass of grain and not be visable. Check on them every couple of days. You see any other colour than white, you throw em out. In the mean time, if you are anxious, use that energy to get into agar:

https://www.shroomery.org/forums/showflat.php/Number/24330957
https://www.shroomery.org/forums/showflat.php/Number/19208976

That way you can get some clean cultures on cheap dishes grown out, throw out anything thats contaminated (and spend as little time and resources in doing so) and get faster colonisation on your spawn as you dont have to wait for spores to germinate and mate.


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Please correct me if I say something wrong!

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OfflineShroomSage
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Registered: 07/13/20
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Last seen: 3 years, 6 months
Re: Advice on Grain Spawn Duration [Re: ManifoldPrime]
    #26822708 - 07/14/20 10:33 AM (3 years, 6 months ago)

Awesome thank you, I will definitely do my reading.


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OfflineRedWolf77
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Registered: 04/11/20
Posts: 11
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Re: Advice on Grain Spawn Duration [Re: ShroomSage]
    #26824690 - 07/15/20 09:01 AM (3 years, 6 months ago)

Good deal bro. keep us posted.


--------------------
REDWOLF


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InvisiblePastywhyteMDiscord
Say hello to my little friend
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Registered: 09/15/12
Posts: 37,809
Loc: Canada Flag
Trusted Cultivator
Re: Advice on Grain Spawn Duration [Re: RedWolf77]
    #26824723 - 07/15/20 09:23 AM (3 years, 6 months ago)

Because of the way they need to be made, PE, APE and PEU spore syringes are often less than axenic. It’s to be expected but most of the time some bacteria or yeast is going to pop up. Spores to grain is hamfisted at the best of times but, with PE variants it’s downright risky.


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Offlinepureshrooming
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Registered: 05/28/18
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Re: Advice on Grain Spawn Duration [Re: Pastywhyte]
    #26824813 - 07/15/20 10:02 AM (3 years, 6 months ago)

It could be a month or more before you see any growth


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