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Shop: North Spore Injection Grain Bag   PhytoExtractum Maeng Da Thai Kratom Leaf Powder

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Offlinemysticalshroom975
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Registered: 07/03/20
Posts: 78
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Agar contaminated? With picture.
    #26821572 - 07/13/20 06:06 PM (3 years, 6 months ago)

There's some discoloration on some blank agar plates.  These plates haven't been inoculated with anything.  They're glass plates that were sterilized at 350F for 20 minutes in an oven.  Once cooled, malt agar was poured in that was sterilized at 10 PSI for 30 minutes. The agar was poured two days ago and they were stored at 80F since then. It's hard to see in the picture, but it appears it is coming from in between the glass and the agar, not the working side of the agar. There's a fair amount of this discoloration but for some reason, I wasn't able to take a good picture that was in focus enough to show it. Do these plates need to be scrapped?


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OfflineEntheoGod
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Registered: 03/06/15
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Re: Agar contaminated? With picture. [Re: mysticalshroom975]
    #26821578 - 07/13/20 06:10 PM (3 years, 6 months ago)

its really hard to tell in this pic if thats a contam or just a particle of dust that got in your agar.  I wouldnt worry until you see more growth.


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Offlinemysticalshroom975
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Registered: 07/03/20
Posts: 78
Last seen: 9 months, 2 days
Re: Agar contaminated? With picture. [Re: EntheoGod]
    #26821595 - 07/13/20 06:17 PM (3 years, 6 months ago)

Yeah its really hard to get a good picture of it but that stuff is all over the plates, some more than others.  Like I said, it looks like something growing in between the agar and the glass, but I don't know what could have survived at 350F.


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